Publication Date:
2003-09-27
Description:
The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme was purified from Drosophila S2cells and consists of two stoichiometric subunits: Dicer-2(DCR-2) and a previously unknown protein that we named R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4. Association with R2D2 does not affect the enzymatic activity of DCR-2. Rather, the DCR-2/R2D2 complex, but not DCR-2 alone, binds to siRNA and enhances sequence-specific messenger RNA degradation mediated by the RNA-initiated silencing complex (RISC). These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Liu, Qinghua -- Rand, Tim A -- Kalidas, Savitha -- Du, Fenghe -- Kim, Hyun-Eui -- Smith, Dean P -- Wang, Xiaodong -- DC02539/DC/NIDCD NIH HHS/ -- New York, N.Y. -- Science. 2003 Sep 26;301(5641):1921-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14512631" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Argonaute Proteins
;
Biotinylation
;
Caenorhabditis elegans/genetics/metabolism
;
Caenorhabditis elegans Proteins/chemistry
;
Cell Line
;
Chemical Precipitation
;
Drosophila Proteins/chemistry/genetics/*isolation & purification/*metabolism
;
Drosophila melanogaster/*genetics/metabolism
;
Electrophoretic Mobility Shift Assay
;
Endoribonucleases/genetics/isolation & purification/*metabolism
;
Kinetics
;
Molecular Sequence Data
;
Mutation
;
Protein Structure, Tertiary
;
RNA Helicases/genetics/*isolation & purification/*metabolism
;
*RNA Interference
;
RNA, Double-Stranded/metabolism
;
RNA, Messenger/metabolism
;
RNA, Small Interfering/*metabolism
;
RNA-Binding Proteins/chemistry/genetics/isolation & purification/*metabolism
;
RNA-Induced Silencing Complex/isolation & purification/metabolism
;
Recombinant Proteins/metabolism
;
Ribonuclease III
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
