Publication Date:
2004-09-18
Description:
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fridman, Eyal -- Carrari, Fernando -- Liu, Yong-Sheng -- Fernie, Alisdair R -- Zamir, Dani -- New York, N.Y. -- Science. 2004 Sep 17;305(5691):1786-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15375271" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Amino Acid Sequence
;
Aspartic Acid
;
Catalytic Domain
;
Crosses, Genetic
;
Flowers/enzymology/genetics
;
Fruit/enzymology/genetics
;
Gene Expression
;
Gene Expression Regulation, Plant
;
Genes, Plant
;
Genetic Complementation Test
;
Lycopersicon esculentum/enzymology/*genetics/growth & development
;
Molecular Sequence Data
;
*Polymorphism, Single Nucleotide
;
*Quantitative Trait Loci
;
Quantitative Trait, Heritable
;
Solanum/enzymology/*genetics
;
Sucrose/metabolism
;
Transcription, Genetic
;
Transformation, Genetic
;
beta-Fructofuranosidase/chemistry/*genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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