ISSN:
0142-2421
Keywords:
chromosome
;
DNA
;
karyotype
;
Giemsa
;
propidium iodide
;
scanning near-field optical microscope
;
SNOM
;
NSOM
;
scanning force microscope
;
SFM
;
AFM
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Karyotypes of human metaphase chromosomes are used to detect genetic defects like deletions or translocations. For these investigations the chromosomes are treated by the trypsin-Giemsa protocol, resulting in a typical banding pattern. These patterns are investigated using conventional light microscopy. Because of the diffraction limit, even the smallest visible band contains 1 million base pairs. We want to improve resolution by using bright-field scanning near-field optical microscopy (SNOM). Images of trypsin-Giemsa-treated chromosomes are presented and compared with conventional light microscopic, scanning force and scanning fluorescence near-field optical microscopic data. For fluorescence investigations, the chromosomes were stained using propidium iodide. To our knowledge, it is the first attempt to investigate G-banded chromosomes by SNOM.© 1997 John Wiley & Sons, Ltd.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
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