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  • Cell & Developmental Biology  (39)
  • Chemical Engineering  (26)
  • Analytical Chemistry and Spectroscopy  (20)
  • 1
    Digitale Medien
    Digitale Medien
    Some morphological and ultrastructural changes in the ependyma of the amputation stump during early regeneration of the tail in the lizard, Anolis carolinensisSupported by grants from the American Cancer Society, the National Institutes of Health and the Multiple Sclerosis Society. (1973)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 140 (1973), S. 257-269 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Light and electron microscope studies indicate that the old ependyma just proximal to the plane of amputation in early lizard tail regenerates shows a sequence of morphological changes which suggests that it as well as the new ependyma growing into the regenerate may play an active role in the initiation and maintenance of early tail regeneration.The old ependyma close to the plane of amputation undergoes hypertrophy and/or hyperplasia causing a partial closure of the central canal and pseudostratification. Its nuclei shift from an original apical position to a basal one. The ependymal processes become more prominent and extend to the pia, a condition not found more rostrally. There is also a significant increase in the amount of Golgi substance and a moderate increase in the rough endoplasmic reticulum. These observations lead to the thought that these cellular changes may be an expression of enhanced secretion and other activities in the old and new ependyma just proximal or distal to the plane of amputation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051400302
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The leucocytes and leucocytopoietic organs of an oligochaete, pheretima indica (Horst) (1929)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 47 (1929), S. 435-478 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The perivisceral fluid of Pheretima indica (Horst) contains five types of leucocytes: lymphocytes, monocytes, granulocytes, lamprocytes, and linocytes. The granulocytes differentiate either from free lymphocytes, from peritoneal epithelial cells lining the leucocytopoietic organs, or from lymphocytes (hemocytoblasts) embedded in these organs. The lymphocyte is a hemocytoblast. The eosinophilic granulocyte is the most numerous of the several types of granulocytes. Morphologically and tinctorially, it resembles the eosinophil of fishes. The eosinophil granule is thought to arise either by a ripening of a basophil granule and to pass through a metachromatic phase during this process, or by being formed immediately without such a ripening process in small hemocytoblasts. The stimulus for the excessive production of eosinophils is thought to be the degree of infection of the leucocytopoietic organs by a species of the gregarine, Monocystis.A series of segmentally arranged leucocytopoietic organs is described for the first time in the oligochaetes. These organs are essentially foldings of the septa and offer sacculations in which leucocytopoiesis may take place.A discussion of the possible phylogeny of the hemocytopoietic organs of the invertebrates and vertebrates is given.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1050470205
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Effects of hypophysectomies and thyroxine replacement upon the initiation of tail regeneration in the lizard, Anolis carolinensisSupported by grants to M. Singer from the American Cancer Society, the National Institutes of Health and the Multiple Sclerosis Society. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 449-461 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: A histological evaluation of the effects of hypophysectomy and throxine therapy in young tail regenerates was carried out in the small iguanid lizard, Anolis c. carolinensis. Hypophysectomy caused a delay but did not inhibit blastema formation. The growth of the ependyma into the wound region was delayed in hypophysioprivic regenerates by about a week.Growth and differentiation of hypophysioprivic regenerates after blastema formation was variable, ranging from virtually no growth to the formation of a differentiated but very small protuberance. However, actual tail elongation was inhibited by hypophysectomy. In those hypophysioprivic regenerates that did show signs of differentiation, muscle groups were poorly defined, scanty in appearance and not as well differentiated as the cartilage tube.Thyroxine treatment in the young hypophysioprivic regenerates stimulated normal growth and normal appearance and differentiation of promuscle and procartilage aggregates as well as the growth of the ependymal tube into the blastema.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051370405
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Role of microtubules in random cell migration: Stabilization of cell polarity (1994)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 27 (1994), S. 88-96 
    Details
    ISSN: 0886-1544
    Schlagwort(e): cell movement ; speed ; persistence time ; colcemid ; alveolar macrophage ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The role of microtubules in random cell migration was investigated using time-lapse videomicroscopy to record in vitro the shape and motile behavior of guinea pig alveolar macrophages before and after disrupting microtubules with colcemid. Cell migration was quantified in terms of directional persistence time and speed. Motility was also correlated with morphological polarity: cells having a single lamellipodal region (monopolar cells) migrated, whereas those lacking a lamellipod (apolar cells) or with opposing lamellipodal regions (bipolar cells) did not migrate. Within 2 hours, colcemid caused a shift in polarity from 80% monopolar cells to 40% monopolar and 40% bipolar cells and a corresponding decrease from 80% to 40% in the fraction of migrating cells. Mean persistence time and speed decreased only slightly (approximately 20%) for those cells (still monopolar) which continued to migrate in the presence of colcemid. Persistence time and speed actually increased for many individual cells, indicating that random migration did not require intact microtubules. We conclude that colcemid treatment destabilizes monopolarity, leading to the gradual loss of monopolarity and consequent inhibition of migration. While a cell remains monopolar, it will continue to migrate even in the absence of intact microtubules, but microtubules are required for the long-term maintenance of cellular monopolarity and, thus, for continued motility. © 1994 Wiley-Liss, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cm.970270110
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Analysis of native neutral glycosphingolipids by combined high performance liquid chromatography/mass spectrometry (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 14 (1987), S. 149-153 
    Details
    ISSN: 0887-6134
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Conditions for normal phase high performance liquid chromatrography-chemical ionization mass spectrometry of underivatized glycosphingolipids with the use of a moving belt transport interface were investigated. Chromatography was performed on a 2 mm ID × 3 cm column packed with 5 μm spherical silica. A gradient of increasing methanol and water in methylene chloride was used to resolve mono-, di-, tri-, and tetraglycosylceramides and sulfatides in less than 15 min. A polyimide belt was used to transport the sample residues to the mass spectrometer where ammonia chemical ionization mass spectrometry was used to obtain spectra. One to 5 μg of each component was sufficient to obtain full spectral scans. Mono- and dihexosylceramide spectra showed [M + H]+ ions of good abundance, while the higher glycosphingolipids have molecular weights that exceed the range of the mass spectrometer utilized. All glycosphingolipids gave ions characteristic of their ceramide, fatty acid, long chain base and carbohydrate components. Sequence information which reflected the relative position of hexose and N-acetylhexosamine residues was also obtained.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bms.1200140403
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Aggregation kinetics in salt-induced protein precipitation (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 1779-1790 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The kinetics of protein aggregation in salt-induced precipitation processes were studied as a function of salt type, salt concentration, temperature and protein concentration. α-chymotrypsin (αCT) was used as a model protein. Stopped-flow turbidimetry was used to monitor the progress of precipitations. Analysis of the linear portions of the turbidity trajectories indicates that temperature and salt concentration effects are related to protein solubility; the protein concentration dependence is well described by the Smoluchowski collision equation. The aggregation kinetics of partially-inhibited αCT exhibit poisoning behavior, underscoring the importance of dimerization and monomer addition in the precipitation of αCT. Solute particle radius distributions determined via dynamic laser light scattering for low salt and supernatant αCT solutions indicated that significant aggregation does not occur in the absence of supersaturation. A detailed population balance model was proposed that accounted for specific and nonspecific interactions and monomer addition. The model is expected to find general application to protein aggregation phenomena, in particular for proteins that have specific quaternary interactions.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690351104
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Effect of inlet conditions on scalar statistics in pipe mixing (1997)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 1947-1954 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Recent experimental validation of theoretical and numerical predictions regarding scalar variance decay in a pipe have revealed that the traditional plug-flow reactor picture of pipe flow omits key physical mechanisms. In particular, the far-field decay exhibits a power law rather than exponential decay. These observations are examined by performing a set of experiments where the manner in which the scalar constituents are introduced into the pipe is varied. Significant differences are noted in the behavior of the variance decay until the very far field is reached, where an invariant power-law form emerges.
    Zusätzliches Material: 14 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690430804
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Simulation of methane partial oxidation over silica-supported MoO3 and V2O5 (1991)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 87-97 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Microkinetic simulations have been carried out to describe the partial oxidation of methane over silica-supported molybdena and vanadia. The objective of this study was to formulate a physically reasonable reaction network to capture the basic aspects of the surface catalytic chemistry and to use this network to link methane oxidation kinetics with the kinetics observed for the oxidation of subsequent gaseous products, i.e., methanol, formaldehyde, and carbon monoxide. The most abundant reactive intermediates on the catalyst surface are suggested to be oxygen and hydroxyl groups. This reaction network successfully predicts the catalytic activities, selectivities, activation energies, and reaction orders observed over silica-supported molybdena and vanadia; and the microkinetic model also semiquantitatively explains the kinetic trends for related catalyst systems and is consistent with surface bonding energetics reported in the literature over various oxide catalysts. The microkinetic reaction network properly reduces to the more simple macrokinetic model reported previously for methane partial oxidation.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690370108
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Analysis and design of metabolic reaction networks via mixed-integer linear optimization (1996)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 42 (1996), S. 1277-1292 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Improvements in bioprocess performance can be achieved by genetic modifications of metabolic control structures. A novel optimization problem helps quantitative understanding and rational metabolic engineering of metabolic reaction pathways. Maximizing the performance of a metabolic reaction pathway is treated as a mixed-integer linear programming formulation to identify changes in regulatory structure and strength and in cellular content of pertinent enzymes which should be implemented to optimize a particular metabolic process. A regulatory superstructure proposed contains all alternative regulatory structures that can be considered for a given pathway. This approach is followed to find the optimal regulatory structure for maximization of phenylalanine selectivity in the microbial aromatic amino acid synthesis pathway. The solution suggests that from the eight feedback inhibitory loops in the original regulatory structure of this pathway, inactivation of at least three loops and overexpression of three enzymes will increase phenylalanine selectivity by 42%. Moreover, novel regulatory structures with only two loops, none of which exists in the original pathway, could result in a selectivity up to 95%.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690420509
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Platelet interaction with poly(ethylene oxide) networks (1990)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 994-1002 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Interpenetrating polymer networks (IPNs) of poly(ethylene oxide) (PEO) and a polyether substituted polysiloxane were synthesized containing variations of PEO content (35 and 65% dry network weight) and PEO systematically varied from low to high molecular weight (2,000, 8,000, and 20,000). Biocompatibility was assessed by measureing 111In platelet deposition in a baboon femoral ex vivo shunt. Mass transfer analysis revealed that platelet transport to PEO/polysiloxane surfaces was kinetically limited and correlated with both PEO content and molecular weight. Differences in material performance were noted particularly after the initial 30 min blood contact period. In mid and high PEO molecular weight networks (8,000 and 20,000), reduced platelet deposition was noted with increased PEO content. In materials of high PEO content (65%), mid and high PEO molecular weight samples had significantly lower levels of platelet adsorption than PDMS (60 min; p〈 0.05) or networks of low PEO molecular weight, coded 2K-65 (30 and 60 min; p 〈 0.05). The lowest level of platelet deposition was noted on 20K-65 networks, less than one platelet per 1,000 μm2.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690360704
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