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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 45 (1973), S. 2428-2429 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillian Magazines Ltd.
    Nature 425 (2003), S. 516-521 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In infections, microbial components provide signals that alert the immune system to danger and promote the generation of immunity. In the absence of such signals, there is often no immune response or tolerance may develop. This has led to the concept that the immune system responds only to ...
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford UK : Blackwell Science Ltd
    Sedimentology 46 (1999), S. 0 
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: There are few descriptions of pre-Quaternary tufa or travertine, yet these deposits have important climatic, hydrological and tectonic implications. In the Badlands of South Dakota, the Late Eocene Chadron Formation contains a variety of tufa (fluvial, lacustrine, paludal and perched springline types), travertine and calcrete (pedogenic and non-pedogenic types). In fluvial channels, tufas and tufa barrages (oncolites, tufa lithoclast conglomerates and phytoherms) are transitional to travertines (pseudostromatolitic boundstone gravels and small pinnacle resurgences with rimstone edges and pools). Lacustrine tufas formed in small ponds adjacent to other types of tufas and are similar to the ‘low-energy, bench-margin facies’ types observed in modern, groundwater-fed, temperate-zone hardwater lakes. Paludal tufas are laterally discontinuous sheets of pisoid-intraclast wackestone that fill palaeotopography. Distal perched springline tufas are mounds of numerous sloping tufa sheets. Concretions and rhizoliths associated with palaeosols are recognized as pedogenic calcretes, whereas non-pedogenic calcretes are variably cemented, vertically oriented conduits for palaeogroundwater discharge that are located in fault zones. These deposits resulted from palaeogroundwater discharge along faults during the Late Eocene. Coeval unroofing of the Black Hills uplift created a regional groundwater flow system through karsted Palaeozoic limestones exposed in the Black Hills. The Chadron Formation is an avulsion-dominated, siliciclastic unit, yet important bodies of carbonate accumulated in it as a result of groundwater processes.
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  • 4
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 38 (2002), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : The proposed removal of Ballville Dam was assessed by (1) using a new Geographic Information Systems (GIS) based method for calculating reservoir sediment storage, (2) evaluating sediment properties and contamination from core data, and (3) assessing downstream impacts from sediment routing calculations. A 1903 (pre-dam) map was manipulated using GIS to recreate the reservoir bathymetry at time of dam construction and used in combination with a detailed 1993 bathymetric survey to calculate sediment volumes and thickness. Reservoir sediment properties and geochemistry were determined from 14 sediment vibracores. Annual sedimentation rates varied from 1.7 to 4.3 g/cm2/yr based on Cesium-137 (137Cs) and Lead-210 (210Pb) geochronology and dated flood layers. The pore fluid geochemistry (Ba, Co, Cu, Mn) of four cores showed surficial enrichments in Cu, while Co and Mn show secondary peaks within the sediments. GIS calculations showed that a designed channel through the former reservoir able to accommodate the 10 percent Probable Maximum Flood (PMF) would require removing approximately 0.35 million m3 of sediment (27 percent of the reservoir fill), either by dredging at a cost of up to $6.3 million or by releasing fine grained sediment downstream. A sediment routing model was applied for the critical 6 km downstream using four cross sections. The sediment routing model predicts that, for flows exceeding minimum Mean Daily Flow (1924 to 1998 data), greater than 90 percent of this sediment would be transported through downstream reaches into Lake Erie (Sandusky Bay).
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  • 6
    ISSN: 1573-4919
    Keywords: beige mouse ; high performance liquid chromatography ; mass spectrometry ; testosterone ; 5α-dihydrotestosterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of neutral glycosphingolipids was examined in primary kidney cell cultures derived from adult male and female beige mutant mice (C57BL/6J;bg j/bg j) with enrichment for proximal tubule cells during preparation of explants and using defined serum-free medium for the culture conditions. Cell proliferated for 7 daysin vitro to provide confluent or nearly confluent monolayers of epithelial-type growth indicative of proximal tubule cells. The malevs female differences in neutral glycosphingolipids seen in the kidneyin vivo were retained in these 7 day cultures. Cultures derived from males contained galacto- and digalactosylceramides whereas those from females did not express these types of glycolipids. Also, male cells had higher ratios of sphingosine: phytosphingosine containing species in Nfa (non-hydroxy fatty acid) globotriaosylceramide and in glucosylceramide than females. The shift in sphingosine: phytosphingosine to male ratios in Nfa globotriaosylceramide and in glucosylceramide could be stimulated in female kidney cells by treatment with 10−5 M testosterone or 5α-dihydrotestosterone. The male-specific expression of neutral glycosphingolipids, then, appears to be stable character of male-type differentiation in mouse kidney that is passed on during proliferation in culture. Female kidney cells retain an ability to respond to androgens with specific changes in neutral glycosphingolipid expression during 7 days of growthin vitro in serum-free conditions, but do not respond with the induction of the male-specific glycolipids galacto-and digalactosylceramides as seenin vivo.
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  • 7
    ISSN: 1573-4986
    Keywords: Fucosidosis ; glycolipid ; SSEA-1 ; Le x (X)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Neutral glycolipids from the brain of a patient with Fucosidosis were analyzed and two complex glycolipids containing five and eight sugars were isolated from the cortical grey matter. These two glycolipids reacted with antibodies recognizing the SSEA-1 [Lex(X)] carbohydrate determinant. SSEA-1 glycolipids are normally expressed in human embryonic brain but are found in only small amounts in postnatal human brain. The accumulation of the two SSEA-1 glycolipids in Fucosidosis brain thus represents a defect which affects the normal developmentally regulated decrease in postnatal, expression of these glycolipids, and may be a contributing factor in the abnormal brain development associated with the disease. Chemical characterization of the two isolated glycolipids by gas chromatographic and mass spectrometric analyses has identified the two glycolipids as lacto-N-fucopentaosylceramide (III) and difucosyl-neolactonorhexaosylceramide.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 14 (1987), S. 149-153 
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conditions for normal phase high performance liquid chromatrography-chemical ionization mass spectrometry of underivatized glycosphingolipids with the use of a moving belt transport interface were investigated. Chromatography was performed on a 2 mm ID × 3 cm column packed with 5 μm spherical silica. A gradient of increasing methanol and water in methylene chloride was used to resolve mono-, di-, tri-, and tetraglycosylceramides and sulfatides in less than 15 min. A polyimide belt was used to transport the sample residues to the mass spectrometer where ammonia chemical ionization mass spectrometry was used to obtain spectra. One to 5 μg of each component was sufficient to obtain full spectral scans. Mono- and dihexosylceramide spectra showed [M + H]+ ions of good abundance, while the higher glycosphingolipids have molecular weights that exceed the range of the mass spectrometer utilized. All glycosphingolipids gave ions characteristic of their ceramide, fatty acid, long chain base and carbohydrate components. Sequence information which reflected the relative position of hexose and N-acetylhexosamine residues was also obtained.
    Additional Material: 8 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 168-171 
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The measurement of plasma free fatty acid turnover and oxidation by means of the isotopic dilution technique using a radiolabeled free fatty acid has proven useful for the in vivo study of fat metabolism in animals. However, because of the ethical considerations regarding the administration of radioisotopes to humans, there is a lack of adequate information regarding the in vivo regulation of free fatty turnover and oxidation in humans, particularly children. Consequently, we have developed a technique using the stable, nonradioactive [1-13C]palmitic acid molecule as free fatty acid tracer to measure plasma free fatty acid metabolism in vivo. We have analysed the isotopic enrichment of plasma palmitate by selected ion monitoring on a gas chromatograph mass spectrometer and we have used an isotope ratio mass spectrometer to determine the much lower isotopic enrichment of the expired CO2. We have found this technique to be safe to administer, to have a high degree of analytical reproducibility, and to yield values in dogs comparable with those obtained using [1-14C]palmitic acid as the tracer.
    Additional Material: 2 Ill.
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  • 10
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Two methods, direct injection-continuous flow fast atom bombardment (DI-CF/FAB) mass spectrometry and micro-high-performance liquid chromatography (μHPLC)-CF/(FAB) mass spectrometry were developed for the clinical analysis of urinary bile acids and their conjugates, specifically for the diagnosis of disorders of bile acid metabolism. The method based upon DI of urine extracts into the CF/FAB flow stream was developed for the rapid screening of large numbers of patient samples. It allows injections to be made at 4 min intervals for high sample throughput. Analyses of standard mixtures of bile salts demonstrate that linear response curves are obtained over more than a hundred-fold concentration range with a minimum detectable amount in the picogram range. Oxo bile salts are identified by reduction with sodium borodeuteride followed by reanalysis for detection of any reduction products. This methodology has been used for the analysis of over 1000 specimens. A reverse-phase μHPLC-CF/FAB mass spectrometric method was also developed for use in the confirmation or further investigation of screening results. With no additional sample preparation this method permits the separation, identification and quantitation of urinary bile salt isomers within a 45 min analysis time.
    Additional Material: 9 Ill.
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