ISSN:
1432-203X
Keywords:
Keywords Rapid-cycling
;
Brassica napus
;
Somatic embryogenesis
;
Secondary embryogenesis
;
Regeneration
;
In vitro Howering
;
AbbreviationsABA: Abscisic acid
;
BAP: 6-Benzyl-aminopurine
;
DAP: Days after pollination
;
2-iP: 6-(γ, γ-dimethlyallyl-amino)purine
;
Kinetin: 6-Furfurylaminopurine
;
MS: Murashige and Skoog
;
SE0: Somatic embryo from seed
;
SE1: First-generation secondary embryo
;
SE2: Second-generation secondary embryo
;
Zeatin: 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5–5) was more conducive to somatic embryogenesis than a higher pH (6 and 7). Embryogenic potential of the seeds was inversely correlated to seed age: about 41–68% of immature seeds between the ages of 14 and 28 days after pollination (DAP) formed somatic embryos compared to 0–11% of the seeds obtained 29–37 DAP. About 54% of the somatic embryos produced secondary embryos after subculturing onto the same medium. The embryogenic potential of the cultures has been maintained on MS basal medium for 2 years (12 generations) without diminution. Up to 75% of the secondary embryos developed into plantlets on MS medium enriched with 10–6 M zeatin, and 40% of these produced flowers when transferred to an optimised flower-induction medium. Viable seeds were produced in self-pollinated in vitro flowers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000268
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