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  • 1
    Electronic Resource
    Electronic Resource
    Isolation, characterization and chromosomal mapping of an actin gene from the primitive red alga Cyanidioschyzon merolae (1995)
    Springer
    Current genetics 28 (1995), S. 484-490 
    Details
    ISSN: 1432-0983
    Keywords: Actin gene ; Cyanidioschyzon merolae ; Pulsed-field gel electrophoresis (PFGE) ; Phylogenetic tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Based on the results of cytological studies, it has been assumed that Cyanidioschyzon merolae does not contain actin genes. However, Southern hybridization of C. merolae cell-nuclear DNA with a yeast actin-gene probe has suggested the presence of an actin gene in the C. merolae genome. In the present study, an actin gene was isolated from a C. merolae genomic library using a yeast actin-gene probe. The C. merolae actin gene has no intron. The predicted actin is composed of 377 amino acids and has an estimated molecular mass of 42003 Da. Southern hybridization indicated that the C. merolae genome contains only one actin gene. This gene is transcribed at a size of 2.4 kb. When Southern hybridization was performed with C. merolae chromosomes separated by pulsed-field gel electrophoresis, a band appeared on unseparated chromosomes XI and XII. A phylogenetic tree based on known eucaryote actin-gene sequences revealed that C. merolae diverged after the division of Protozoa, but before the division of Fungi, Animalia and Chlorophyta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310820
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  • 2
    Electronic Resource
    Electronic Resource
    A novel mutation occurring in the PHO80 gene suppresses the PHO4 c mutations of Saccharomyces cerevisiae (1992)
    Springer
    Current genetics 21 (1992), S. 95-99 
    Details
    ISSN: 1432-0983
    Keywords: PHO ; Saccharomyces ; Protein-protein interaction ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated suppressors of a PHO4 c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isoleucine for methionine 42 of the Pho80 protein. Substitution of valine (PHO80-3) or leucine (PHO80-4) for the same methionine by site-directed mutagenesis also suppressed PHO c. Suppression by PHO80-2) did show some allele specificity. From these results we were able to delimit the region of PHo80 which may interact with the Pho4 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318466
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of a marker gene cassette which is repeatedly usable for gene disruption in yeast (1995)
    Springer
    Current genetics 27 (1995), S. 293-297 
    Details
    ISSN: 1432-0983
    Keywords: Gene disruption ; Site-specific recombination ; Yeast plasmid ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A disruption cassette has been constructed containing the LEU2 gene flanked by directly repeated sitespecific recombination sites of the yeast plasmid, pSB3, which resembles the 2 μm DNA of Saccharomyces cerevisiae. A disruption constructed by inserting this DNA fragment acquires a Leu+ phenotype, which can be easily removed by expressing the FLP-PSB3 gene encoding the site-specific recombinase of pSB3. A test was made using a Schizosaccharomyces pombe host. The ura4 + gene of S. pombe was replaced with the ura4::LEU2 gene constructed by inserting the disruption cassette into the ura4 + gene. Then, the FLP-pSB3 gene driven by the nmt1 + promoter was introduced into this disruptant. Upon de-repression of the nmt1 promoter by removing thiamine from the medium, the rate of appearance of Leu- was increased. As expected the ura4 + locus underwent a structural change. Thus, the FLP-pSB3 protein and its target site can function adequately in S. pombe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352095
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  • 4
    Electronic Resource
    Electronic Resource
    A dominant interfering mutation in RAS1 of Saccharomyces cerevisiae (1993)
    Springer
    Molecular genetics and genomics 241 (1993), S. 280-286 
    Details
    ISSN: 1617-4623
    Keywords: Yeast RAS ; RAS-CAMP pathway ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A mutant allele of RAS1 that dominantly interferes with the wild-type Ras function in the yeast Saccharomyces cerevisiae was discovered during screening of mutants that suppress an ira2 disruption mutation. A single amino acid substitution, serine for glycine at position 22, was found to cause the mutant phenotype. The inhibitory effect of the RAS1 Ser22 gene could be overcome either by overexpression of CDC25 or by the ira2 disruption mutation. These results suggest that the RAS1Ser22 gene product interferes with the normal interaction of Ras with Cdc25 by forming a dead-end complex between Ras1Ser22 and Cdc25 proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284679
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of temperature-sensitiveplc1 mutants of the yeastSaccharomyces cerevisiae (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 148-156 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Phosphoinositide-specific phospholipase C ; Temperature-sensitive mutant ; PLC1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ThePLC1 gene of the yeastSaccharomyces cerevisiae has been discovered to encode a homolog of mammalian phosphoinositide-specific phospholipase C (PLC). Five temperature-sensitiveplc1 mutants were isolated by in vitro mutagenesis with subsequent plasmid shuffling. All of the amino acid substitutions that caused a temperature-sensitive growth phenotype were located in the X or the Y region, both of which are conserved among PLC isoenzymes. The PLC activity of all products of mutantplc1 genes was dramatically lower than that of the wild-type product, indicating that PLC activity itself is important for cell growth. At the restrictive temperature,plc1 mutant cells ceased growth at random times during the cell cycle, a result that suggests thatPLC1 is required at several or all stages of the cell cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705644
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  • 6
    Electronic Resource
    Electronic Resource
    A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a counterpart of the Drosophila melanogaster diphenol oxidase A2 gene, Dox-A2 (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 146-152 
    Details
    ISSN: 1617-4623
    Keywords: Key words Saccharomyces cerevisiae ; Cell cycle ; Dox-A2 ; NIN1 ; P91A ; SUN2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  NIN1 is an essential gene for growth of the yeast Saccharomyces cerevisiae and was recently found to encode a component of the regulatory subunit of the 26S proteasome. The nin1-1 mutant is temperature sensitive and its main defect is in G1/S progression and G2/M progression at non-permissive temperatures. One of the two multicopy suppressors of nin1-1, SUN2 (SUppressor of Nin1-1), was found to encode a protein of 523 amino acids whose sequence is similar to those of Drosophila melanogaster diphenol oxidase A2 and the mouse mast-cell Tum- transplantation antigen, P91A. The C-terminal half of Sun2p was found to be functional as Sun2p at 25° C, 30° C, and 34° C but not at 37° C. The open reading frame (ORF) of the Drosophila diphenol oxidase A2 gene (Dox-A2) was obtained from a lambda phage cDNA library using the polymerase chain reaction technique. The Dox-A2 ORF driven by the TDH3 promoter complemented the phenotype of a strain deleted for sun2. This Dox-A2-dependent strain was temperature sensitive and accumulated dumb-bell-shaped cells, with an undivided nucleus at the isthmus, after temperature upshift. This morphology is similar to that of nin1-1 cells kept at a restrictive temperature. These results suggest that SUN2 is a functional counterpart of Dox-A2 and that these genes play a pivotal role in the cell cycle in each organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172912
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  • 7
    Electronic Resource
    Electronic Resource
    Dominant mutant alleles of yeast protein kinase geneCDC15 suppress the lte1 defect in termination of M phase and genetically interact withCDC14 (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 176-185 
    Details
    ISSN: 1617-4623
    Keywords: Key words Saccharomyces cerevisiae ; Cell cycle ; LTE1 ; CDC15 ; CDC14
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  LTE1 encodes a homolog of GDP-GTP exchange factors for the Ras superfamily and is required at low temperatures for cell cycle progression at the stage of the termination of M phase in Saccharomyces cerevisiae. We isolated extragenic suppressors which suppress the cold sensitivity of lte1 cells and confer a temperature-sensitive phenotype on cells. Cells mutant for the suppressor alone were arrested at telophase at non-permissive temperatures and the terminal phenotype was almost identical to that of lte1 cells at non-permissive temperatures. Genetic analysis revealed that the suppressor is allelic to CDC15, which encodes a protein kinase. The cdc15 mutations thus isolated were recessive with regard to the temperature-sensitive phenotype and were dominant with respect to suppression of lte1. We isolated CDC14 as a low-copy-number suppressor of cdc15-rlt1. CDC14 encodes a phosphotyrosine phosphatase (PTPase) and is essential for termination of M phase. An extra copy of CDC14 suppressed the temperature sensitivity of cdc15-rlt1 cells, but not that of cdc15-1 cells. In addition, some residues that are essential for the Cdc14 PTPase activity were found to be non-essential for the suppression. These results strongly indicate that Cdc14 possesses dual functions; PTPase activity is needed for one function but not for the other. We postulate that the cooperative action of Cdc14 and Cdc15 plays an essential role in the termination of M phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172916
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  • 8
    Electronic Resource
    Electronic Resource
    PHO85, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyces cerevisiae (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 162-164 
    Details
    ISSN: 1617-4623
    Keywords: CDC28 ; Phosphate regulation ; PHO85 ; Protein kinase ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The product of the PHO85 gene, which encodes one of the negative regulatory factors of the PHO system in Saccharomyces cerevisiae, shows significant amino acid sequence homology with the CDC28 protein kinase. However, overexpressing PHO85 did not suppress the temperature sensitive phenotype of the cdc28-1 mutation. The nucleotide sequence of the PHO85 gene strongly suggests the presence of an intron near the sequence encoding the N-terminal region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340196
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  • 9
    Electronic Resource
    Electronic Resource
    Two new genes controlling the constitutive acid phosphatase synthesis in Saccharomyces cerevisiae (1975)
    Springer
    Molecular genetics and genomics 141 (1975), S. 81-83 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two new classes of mutants, phoF and phoG, lacking the constitutive acid phosphatase activity, were isolated. They both complemented each other and the phoC mutation. No linkage was detected among these three complementary genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332380
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  • 10
    Electronic Resource
    Electronic Resource
    Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae (1975)
    Springer
    Molecular genetics and genomics 143 (1975), S. 65-70 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The phoE locus, one of the loci in which mutations lack the activity for repressible acid phosphatase, was found to be the structural gene for the enzyme by examining the enzymic characteristics of repressible acid phosphatase activity using cell extracts prepared from the leaky phoE mutants, the PHOE revertants and the PHOE recombinants between the different phoE mutants. Other evidence which strongly suggests that the phoC locus is coding for the constitutive acid phosphatase was obtained by a similar investigation. Although the phoC and phoE loci are tightly linked, they were separable by meiotic recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269421
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