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  • 1
    Electronic Resource
    Electronic Resource
    Procedures for the production and separation of H and M antigens in histoplasmin: Chemical and serological properties of the isolated products (1977)
    Springer
    Mycopathologia 61 (1977), S. 131-141 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two strains of Histoplasma capsulatum were required to prepare maximum yields of H and of M antigen from histoplasmin. The antigens were separated and partially purified by a series of procedures yielding an overall recovery of 70 to 90% of the individual antigens. Stable products suitable for use as reference products were obtained when the final purification step employed DEAE-cellulose with phosphate buffer elution at increasing molarity and decreasing pH. A final step of purification of each antigen with slab acrylamide gel electrophoresis gave products which were highly reactive and specific in a variety of serological tests with sera from persons with proven cases of histoplasmosis and with natural infections of heterologous deep mycoses. These antigens were maximally active at concentrations of 2 to 16 μg protein in the complement fixation, capillary precipitin, microimmunodiffusion, or immunoelectrophoresis tests; 0.5 μg gave a maximum delayed cutaneous hypersensitivity reaction in homologously infected animals and caused no appreciable reaction in control animals. Although these antigens appeared to be specific when tested with sera from persons with natural infections, the M and H antigens demonstrated the presence of an additional antigen reacting with sera of rabbits immunized with cell membrane and cell particulate fractions of Blastomyces dermatitidis. After purification by electrophoresis, both the H and M antigens of some preparations showed some decomposition and loss of reactivity after storage at 5 C for more than six months. The overall results suggest that the purified H and M antigens of Heiner (12) have multiple serological reactivity and may function in precipitin reactions, complementfixing reactions, hemagglutination of formalin-fixed goose red blood cells, and as antigens for delayed cutaneous tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00468007
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  • 2
    Electronic Resource
    Electronic Resource
    Biochemical and serological characteristics of soluble yeast phase antigens of Histoplasma Capsulatum (1979)
    Springer
    Mycopathologia 67 (1979), S. 3-16 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Soluble antigens of whole yeast-phase cells were extracted with a 0.1 M phosphate buffer containing 0.1 M sodium chloride and 0.02% iodoacetate. After being separated by differential filtration into fractions less than or greater than 50,000 daltons, these antigens were purified by molecular sieve and chromatographic separations on ionic exchange resins. Two high molecular weight fractions obtained from diethylaminoethyl-cellulose (DEAE) at pH 8.0 and 7.0 with tris (hydroxymethyl) aminomethane (Tris) buffer were M antigens; those obtained at pH 4.0 and 4.0 with salt were H antigens. The four fractions had protein to carbohydrate ratios of 7.3, 14.0, 8.4, and 6.5 respectively, and all had essentially the same amino acid composition with no methionine and tyrosine and little histidine, arginine, phenylalanine and lysine. They had high concentrations of glucose, less mannose and traces of galactose. The low molecular weight fractions had the new complex ‘Y antigen’, M antigen, and H antigen with protein to carbohydrate ratios of 1.4, 1.4 and 0.3 respectively. The amino acid and sugar composition of Y antigen strongly resembled the composition of the low molecular weight H and M antigens. Unlike the high molecular weight antigens, these low molecular weight antigens had methionine in relatively high concentrations; they had the same sugars as their respective high molecular weight counterparts. The yeast phase antigens differed from their respective mycelial counterparts in the following ways: glucose was the major sugar in the yeast phase with less amounts of mannose and traces of galactose, whereas in the mycelial antigens, mannose was the major sugar, with lesser amounts of galactose, glucose, and hexosamine. The H and M antigens of the yeast phase had high concentrations of glycine and alanine, whereas in the mycelial phase, these antigens had high concentrations of threonine and proline; the H and M antigens of the yeast phase had 5 to 16 times the protein to carbohydrate ratio observed for the same antigens of histoplasmin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436234
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  • 3
    Electronic Resource
    Electronic Resource
    Acid glycosaminoglycans of mouse neuroblastoma C1300 cells (1974)
    Springer
    Journal of molecular histology 6 (1974), S. 1-5 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis Cultured mouse neuroblastoma C1300 cells were examined for acid glycosaminoglycans using the Alcian Blue and periodic acid-Schiff staining techniques. It was found that the cells contained hyaluronidase-resistant sulphated glycosaminoglycans; hyaluronic acid, chondroitin sulphate, and sialoglycoproteins were not demonstrated. These properties are held in common with foetal mouse brain spongioblasts in culture. In contrast to the latter cells, but in common with some peripheral neuronsin vivo, C1300 cells were stained by the periodic acid-Schiff technique for neutral polysaccharides. The results are discussed in relation to the poor adhesive properties of neuroblastoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01011533
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  • 4
    Electronic Resource
    Electronic Resource
    Glycosaminoglycans of disaggregated foetal mouse brain tissue cultures (1973)
    Springer
    Journal of molecular histology 5 (1973), S. 547-556 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis Disaggregated foetal mouse brain tissue cultures were examined for glycosaminoglycans using Alcian Blue and periodic acid-Schiff staining techniques. It was found that spongioblasts (neuron and glial cell precursors) were rich in sulphated glycosaminoglycans, while astrocytes contained little or no sulphated polymers. The chief acid glycosaminoglycans of the brain reportedin vivo, hyaluronic acid, chondroitin sulphate and sialic acid-bearing polymers, were not demonstrated in the mouse brain cultures. There was a decline in glycosaminoglycan content over two weeks in culture, but during the corresponding periodin vivo an increase has been reported. These deficiencies are possibly correlated with the failure of the cultures to myelinate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012060
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  • 5
    Electronic Resource
    Electronic Resource
    Fabrication of porous iron using sucrose (1997)
    Springer
    Journal of materials science 16 (1997), S. 1437-1440 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018529915151
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  • 6
    Electronic Resource
    Electronic Resource
    Combined gas chromatography-infra-red spectroscopy for the determination of propanediol in acyclovir cream (1996)
    Springer
    Chromatographia 42 (1996), S. 305-308 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography-IR spectroscopy ; Propanediol ; Acyclovir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple procedure is described for the determination of the 1,2-propanediol (propylene glycol) content of cream formulations containing the antiviral drug Acyclovir. Analysis of a solvent extract by gas chromatography coupled to an infra-red spectrometer enables the target analyte to be positively identified and an estimate of its concentration to be made. This application utilises the newer Tracer cryogenic deposition interface for gas chromatography and infra-red spectroscopy. This offers an alternative and complementary analytical strategy to gas chromatography coupled to mass spectrometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02290314
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  • 7
    Electronic Resource
    Electronic Resource
    Comparison of the histochemical and contractile properties of human triceps surae (1992)
    Springer
    Medical & biological engineering & computing 30 (1992), S. 600-604 
    Details
    ISSN: 1741-0444
    Keywords: Contractile properties ; Fibre types ; Human triceps surae ; Muscle contraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The purpose of the study was to compare the contractile properties determined from an electrically stimulated twitch with histochemically determined fibre type parameters of the human triceps surae. Muscle samples were obtained from the medial head of the gastrocnemius of ten male athletes. Ages ranged from 20 to 29 years. Muscle samples from the belly of the medial gastrocnemius muscle were obtained using the needle biopsy technique. The samples were treated histochemically for myosin ATPase to classify the fibres as either slow twitch (ST) or fast twitch (FT) and to determine fibre areas. Surface electrical stimulation was used to determine muscle twitch parameters. The contractile variables of the muscle twitch were latency (L), time to peak force (TPF), peak force (PF), half-contraction time (1/2 CT) and half-relaxation time (1/2 RT). Backward elimination procedures for dependent variables were used to determine which contractile properties best represented the histochemical profile of the muscles. Prediction formulas were developed for FT and ST percentages (R2=0·98, p〈0·001), relative area percentage (R2=0·87, p〈0·001), and ST area (R2=0·85, p〈0·01). It was concluded that the use of the electrotensiometer (ETM) protocol was a valid testing procedure when studying physiological relationships of histochemical properties in intact human skeletal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02446791
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  • 8
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    Influence of solution chemistry and surface condition on the critical inhibitor concentration for solutions typical of hot potassium carbonate CO2 removal plant (2007)
    Springer
    Details
    Publication Date: 2007-09-01
    Print ISSN: 0022-2461
    Electronic ISSN: 1573-4803
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Published by Springer
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  • 9
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    Combined gas chromatography-infra-red spectroscopy for the determination of propanediol in acyclovir cream (1996)
    Springer
    Details
    Publication Date: 1996-03-01
    Print ISSN: 0009-5893
    Electronic ISSN: 1612-1112
    Topics: Chemistry and Pharmacology
    Published by Springer
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  • 10
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    Intracellular bacteria associated with the ascidian Ecteinascidia turbinata : phylogenetic and in situ hybridisation analysis (2003)
    Springer
    Details
    Publication Date: 2003-07-01
    Print ISSN: 0025-3162
    Electronic ISSN: 1432-1793
    Topics: Biology
    Published by Springer
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