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  • Springer  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Immobilization of protoplasts by anchoring to microcarriers (1982)
    Springer
    Plant cell reports 1 (1982), S. 151-153 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Datura innoxia cell suspension-derived protoplasts were anchored to Cytodex 1 microcarriers pre-swollen in buffered concanavalin A. As many as 34 protoplasts were estimated to attach per microcarrier, in comparison to a potential 47 as determined from a model based on random anchorage. Fluorescein diacetate was used as localizing agent as well as to assess viability. When included in the swelling medium fluorescence was observed almost instantaneously, first in the protoplast at its interface with the microcarrier, and later throughout the cytoplasm. However, the dye was not conjugated with the lectin, and leakage eventually resulted in fluorescence also of non-anchored protoplasts. Fluorescein-labelled concanavalin A on the other hand permitted detection of microcarriers but not of anchored protoplasts, suggesting the use of differentially fluorescing microcarriers, as an aid in identification of fusion partners.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269185
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition and promotion by abscisic acid of growth in Spirodela (1969)
    Springer
    Planta 85 (1969), S. 157-159 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of different concentrations of abscisic acid on the growth of Spirodela oligorrhiza has been studied. Abscisic acid effectively permanently arrests growth at concentrations down to 10-1 mg/l (one part per 10 million). Normal growth tends to be resumed at concentrations of 10-2 and 10-3 mg/l between 9 and 12 days after treatment. A concentration of 10-8 mg/l, however, results in a significant increase in dry weight at both 9 and 12 days after introduction to the culture medium. It is suggested that the resumption of growth 12 days after treatment at those concentrations which inhibit growth up to 9 days, is due to a possible progressive inactivation of abscisic acid resulting in a lowering of its concentration to a level that is promotive. Sterile plants must be used since abscisic acid has shown no effect whatsoever in controlling growth of Saprolegnia, a water-mould of the Phycomycetes to which Spirodela apparently is the host.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388545
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  • 3
    Electronic Resource
    Electronic Resource
    Avoidance of precipitation and carbohydrate breakdown in autoclaved plant tissue culture media (1991)
    Springer
    Plant cell reports 10 (1991), S. 115-119 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The extent of breakdown of fructose and glucose derived from sucrose in the medium of Murashige and Skoog (1962) during autoclaving was investigated by polarographic measurement. Although not present in the original MS medium but often used in place of FeSO4 + Na2-EDTA, FeNa-EDTA was found to be primarily responsible for catalyzing the breakdown of these monosaccharides. It would therefore be good practice to autoclave FeNa-EDTA separate from the carbohydrate constituents of the medium in order to reduce the formation of toxic substances derived from the latter's breakdown. Autoclaving FeNa-EDTA separately has the additional advantage of preventing precipitation of certain micronutrient elements. Further precipitation can be avoided by autoclaving FeNa-EDTA and KH2PO4 together, but separately, from other components of the medium. By eliminating precipitation and minimizing the breakdown of monosaccharides during autoclaving, it is possible to improve the quality of the medium without resorting to sterilization by filtering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232040
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on isolated starch-containing (Vicia faba) and starch-deficient (Allium cepa) guard cell protoplasts (1978)
    Springer
    Planta 143 (1978), S. 33-39 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Allium ; Fusicoccin ; Guard cell protoplasts ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Guard cell protoplasts from starch-containing Vicia faba and starch-deficient Allium cepa stomata were isolated, stabilized and recovered with an efficiency — in relation to the potential yield — of approx. 62% and 77%, respectively. In vitro, guard cell protoplasts (GCP) respond to abscisic acid and fusicoccin by respectively contracting and swelling, that is, decreasing or increasing in diameter by about 15% and more in comparison to the control. This in vitro response correlates with, but is more than 4 times as rapid as, the in vivo response of the stomata. Among the advantages presented by working with isolated GCPs are: greater sensitivity in response; freedom from influences of cuticular ridges, cell walls, subsidiary cells, and epidermal cells; and direct and parallel comparisons of starch-containing and starch-deficient GCP systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389049
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  • 5
    Electronic Resource
    Electronic Resource
    Cyanide-initiated oxygen consumption in autoclaved culture medium containing sugars (1989)
    Springer
    Plant cell reports 8 (1989), S. 90-92 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The consumption of oxygen initiated by KCN in an autoclaved sugar-containing rinse medium with protoplasts is described. The effect of autoclaving on several sugars was examined. Fructose solutions, followed in decreasing order by glucose, sucrose and sorbitol, were found to contain the largest amount of degraded products that could react with oxygen in the presence of KCN. Mannitol was found to be stable under the autoclaving conditions used in this investigation. KCN generally has an inhibitory effect on respiration, but in some plant tissues, respiration is stimulated by it. Under certain circumstances the degradation artefact described here may confuse interpretation of the results of respiration measurements. The use of autoclaved media containing sugars should be avoided in respiration studies that involve the application of KCN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716846
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of synthetic cytokinins on levels of endogenous cytokinins and respiration patterns ofBeta vulgaris cells in suspension (1991)
    Springer
    Journal of plant growth regulation 10 (1991), S. 197-199 
    Details
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Respiration patterns and growth of cytokinin-dependent cell suspensions ofBeta vulgaris L., precultured in media with or without three different synthetic cytokinins [benzyladenine (BA), kinetin (KIN), and thidiazuron (TDA)], were compared. The content of endogenous cytokinins, especially zeatin and isopentenyladenine, as well as the dry mass yield, were dependent on the kind of synthetic cytokinin present in the culture medium and decreased in the following order: thidiazuron, kinetin, benzyladenine, no cytokinin. The apparent capacity of the alternative pathway, as measured after blocking of the cytochrome pathway by cyanide, was inversely proportional to the content of endogenous cytokinins. Some synthetic cytokinins (e.g., benzyladenine), when exogenously applied, are known to inhibit selectively the alternative pathway. However, this does not necessarily imply that the mechanism of action of endogenous cytokinins on the respiration pattern is limited to a single effect on the alternative pathway. Multiple effects on oxidative processes cannot be excluded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279334
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  • 7
    Electronic Resource
    Electronic Resource
    Structure and development of sieve areas in leaf veins ofWelwitschia (1973)
    Springer
    Protoplasma 76 (1973), S. 23-34 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sieve areas inWelwitschia are essentially similar to those of coniferous sieve cells, consisting of groups of plasmalemma-lined pores, which are joined in the middle of the wall by a median cavity. The median cavity contains membranes which apparently are connected with aggregates of endoplasmic reticulum bordering the sieve areas. The median cavity is formed through union of smaller median enlargements, the median nodules, each initially associated with a plasmodesma, during perforation of the young sieve area. Callose platelets are not associated with developing pores. All fully-developed pores were lined with callose. The sieve cells are connected with only one other cell type, the albuminous cell. On the sieve-cell side of the wall these connections are similar to sievearea pores, on the albuminous-cell side to plasmodesmata. These connections are also characterized by median enlargements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279670
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  • 8
    Electronic Resource
    Electronic Resource
    Structure and development of the sieve-cell protoplast in leaf veins ofWelwitschia (1973)
    Springer
    Protoplasma 76 (1973), S. 1-21 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue of one-year-old leaves ofWelwitschia mirabilis was fixed in glutaraldehyde and postfixed in osmium tetroxide for electron microscopy. Mature sieve cells contain nuclei composed of peripherally-distributed chromatin material and an intact envelope with pores. During sieve-cell development many mitochondria become closely associated spatially with the nucleus. In addition to a nucleus and mitochondria, the mature, plasmalemma-lined sieve cell contains plastids and abundant smooth endoplasmic reticulum, which generally occurs in massive aggregates at the sieve areas. Dictyosomes and ribosomes are lacking and a tonoplast is not discernible in mature sieve cells. P-protein is not present at any stage of development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279669
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  • 9
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    Studies on isolated starch-containing (Vicia faba) and starch-deficient (Allium cepa) guard cell protoplasts (1978)
    Springer
    Details
    Publication Date: 1978-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 10
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    Inhibition and promotion by abscisic acid of growth in Spirodela (1969)
    Springer
    Details
    Publication Date: 1969-06-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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