Abstract
Guard cell protoplasts from starch-containing Vicia faba and starch-deficient Allium cepa stomata were isolated, stabilized and recovered with an efficiency — in relation to the potential yield — of approx. 62% and 77%, respectively. In vitro, guard cell protoplasts (GCP) respond to abscisic acid and fusicoccin by respectively contracting and swelling, that is, decreasing or increasing in diameter by about 15% and more in comparison to the control. This in vitro response correlates with, but is more than 4 times as rapid as, the in vivo response of the stomata. Among the advantages presented by working with isolated GCPs are: greater sensitivity in response; freedom from influences of cuticular ridges, cell walls, subsidiary cells, and epidermal cells; and direct and parallel comparisons of starch-containing and starch-deficient GCP systems.
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Abbreviations
- ABA:
-
abscisic acid
- FC:
-
fusicoccin
- ECP, MCP, and GCP:
-
epidermal, mesophyll, and guard cell protoplasts, respectively
- PPV:
-
packed protoplast volume
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Schnabl, H., Bornman, C.H. & Ziegler, H. Studies on isolated starch-containing (Vicia faba) and starch-deficient (Allium cepa) guard cell protoplasts. Planta 143, 33–39 (1978). https://doi.org/10.1007/BF00389049
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DOI: https://doi.org/10.1007/BF00389049