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  • 1
    Digitale Medien
    Digitale Medien
    Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland ofBombyx mori (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1167-1171 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Silkworm ; Bombyx mori ; silk gland ; mRNA ; complementary DNA ; fibroin light chain ; molecular cloning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm,Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01951711
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Tagging studies on the growth of the sea hare Aplysia kurodai on an intertidal rocky shore (1975)
    Springer
    Marine biology 32 (1975), S. 389-395 
    Details
    ISSN: 1432-1793
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A total of 1,427 juvenile Aplysia kurodai were tagged and released during spring tides in January and February, 1973, on the rocky shore of Nabeta Bay, Japan. Recapture, weighing and release were repeated within a defined observation area during 10 spring tides from late January to early June. Recapture rates decreased with time and became significantly low after the end of the spawning season (March and April in this area). Of 1,427 individuals released, 550 (ca. 39%) were never recaptured. Only 68 individuals (ca. 5%) were recaptured 5 to 10 times during the observation period. The growth curves of these 68 individuals displayed a common growth pattern of body weight increasing almost linearly, attaining a maximum during the spawning season and then decreasing gradually. The maximum weight varied fairly widely from one individual to another. Laboratory measurements indicated a growth pattern similar to that observed in the field, and a fairly remarkable daily fluctuation in body weight was observed under laboratory conditions. The described tagging-recapture method, with slight modifications, should prove useful for future studies on the life history and ecology of A. kurodai.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00388996
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Carnosine-like immunoreactivity in the primary olfactory neuron of the rat (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 298-300 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Carnosine ; neurotransmitter ; olfactory bulb ; primary olfactory neuron ; immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Using the peroxidase-antiperoxidase immunohistochemical technique, carnosine-like immunoreactivity was demonstrated to localize specifically within the primary olfactory neuron.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01945557
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Analgesic dipeptide, L-Tyr-D-Arg (D-kyotorphin) induces Met-enkephalin release from guinea-pig striatal slices (1982)
    Springer
    Cellular and molecular life sciences 38 (1982), S. 1344-1345 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Kyotorphin (L-Tyr-L-Arg), an analgesic dipeptide isolated from the bovine brain, and its analogue, L-Tyr-D-Arg (D-kyotorphin), have a naloxone-reversible analgesic effect. Both peptides (10−5 M) induced an approximately 4-fold increase of the basal release of Met-enkephalin from striatal slices. Therefore they may produce their analgesic effects through release of Met-enkephalin. The stronger in vivo effect of D-kyotorphin may be explained by its resistance to degradation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01954941
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Computer simulations for direct conversion of the HF electromagnetic wave into the upper hybrid wave in ionospheric heating experiments (1998)
    Springer
    Annales geophysicae 16 (1998), S. 1251-1258 
    Details
    ISSN: 0992-7689
    Schlagwort(e): Ionosphere (ionospheric irregularities; plasma waves and instabilities; wave-particle interactions).
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract Excitation of upper hybrid waves associated with the ionospheric heating experiments is assumed to be essential in explaining some of the features of stimulated electromagnetic emissions (SEE). A direct conversion process is proposed as an excitation mechanism of the upper hybrid waves where the energy of an obliquely propagating electromagnetic pump wave is converted into the electrostatic upper hybrid waves due to small-scale density irregularities. We performed electromagnetic particle-in-cell simulations to investigate the energy conversion process in the ionospheric heating experiments. We studied dependence of the amplitude of the excited wave on the propagation angle of the pump wave, scale length of the density irregularity, degree of the irregularity, and thermal velocity of the plasma. The maximum amplitude is found to be 37% of the pump amplitude under an optimum condition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s00585-998-1251-y
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    ”In vivo cryotechnique” in combination with replica immunoelectron microscopy for caveolin in smooth muscle cells (1999)
    Springer
    Histochemistry and cell biology 112 (1999), S. 443-445 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract  A novel ”in vivo cryotechnique” with replica immunoelectron microscopy was developed for detecting caveolin localization on replica membranes prepared directly from living smooth muscle cells. After quick-freezing mouse duodenal walls by our ”in vivo cryotechnique”, the specimens were prepared for freeze-fracture and deep-etch replica membranes. Then they were treated with 5% SDS and 0.5% collagenase to keep some antigens on the replica membranes. The immunogold method could be used to clarify the localization of the caveolin antigen in relation to three-dimensional ultrastructures of living smooth muscle cells. Our new cryotechnique can provide native organization of functional molecules in living cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004180050426
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Dystrophin in rod spherules; submembranous dense regions facing bipolar cell processes (1997)
    Springer
    Histochemistry and cell biology 108 (1997), S. 243-248 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract  It is known that the retina contains the protein dystrophin in the ribbon synapse, but the ultrastructural analysis is not yet fully elucidated. Our previous study reported that dystrophin is localized under the rod cell membranes in rat retinas. In the present study, we have investigated the relationship between dystrophin-rich regions of rod cell membranes and other neuronal processes in mouse retinas with a monoclonal antibody raised against the human dystrophin C-terminus. Immunoblotting, immunofluorescence stainings, and immunoelectron microscopy were employed. Immunoblotting analysis indicated that mouse retinas possessed some of the dystrophin isoforms of approximately 260 kDa, 140 kDa, and 70 kDa molecular weight. Confocal images showed a punctate appearance in the outer plexiform layer, as previously described. Immunoelectron microscopy showed that dystrophin immunoreactive products were always observed at submembranous dense regions of the rod spherule abutting bipolar processes. These results suggest that retinal dystrophin may be closely involved in signal transmission from rods to bipolar cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004180050164
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Organic-inorganic combined polymer: a copolymer of dichlorobenzene and titanium alkoxide (1990)
    Springer
    Journal of materials science 9 (1990), S. 203-204 
    Details
    ISSN: 1573-4811
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00727716
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    A histochemical method for the demonstration of unsaturated lipids (1991)
    Springer
    Journal of molecular histology 23 (1991), S. 91-99 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary, unsaturated lipids were visualized as distinct reaction products coloured various shades of brown and black. The reaction products are easier to see and the method is more efficient than the peracetic acid-Schiff method.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01047113
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Numerical modeling of the nocturnal urban boundary layer (1989)
    Springer
    Boundary layer meteorology 49 (1989), S. 77-98 
    Details
    ISSN: 1573-1472
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract A numerical case study with a second-order turbulence closure model is proposed to study the role of urban canopy layer (UCL) for the formation of the nocturnal urban boundary layer (UBL). The turbulent diffusion coefficient was determined from an algebraic stress model. The concept of urban building surface area density is proposed to represent the UCL. Calculated results were also compared with field observation data. The height of the elevated inversion above an urban center was simulated and found to be approximately twice the average building height. The turbulent kinetic energy k, energy dissipation rate ε, and turbulence intensities 〈u 2〉 and 〈w 2〉 increase rapidly at the upwind edge of the urban area. The Reynolds stress 〈uw〉 displayed a nearly uniform profile inside the UBL, and the vertical sensible heat flux 〈wθ〉 had a negative value at the inversion base height. This indicates that the downward transport of sensible heat from the inversion base may play an important role in the formation of the nocturnal UBL.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00116406
    Standort Signatur Erwartet Verfügbarkeit
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