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  • DNA stabilized  (1)
  • Gene expression (transient)  (1)
  • cis-platin  (1)
  • Springer  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Thermochemical Passivation of DNA-Stabilized Q-Cadmium Sulfide Nanoparticles (2000)
    Springer
    Journal of cluster science 11 (2000), S. 359-372 
    Details
    ISSN: 1572-8862
    Schlagwort(e): cadmium sulfide ; quantum dots ; DNA stabilized
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract In this work, the conditions required to transform quantum-confined cadmium sulfide (Q-CdS) nanoparticles stabilized by calf thymus deoxyribonucleic acid from deep trap photoluminescent states to “band edge”-type luminescence are probed. The presence of fivefold excess sulfide relative to cadmium concentration during cluster synthesis, followed by mild heating at 80°C, results in the desired transformation of the Q-CdS emission spectrum. We also indirectly analyze the accompanying structural changes in the polymeric stabilizer, accomplished in this case by use of well-known spectrofluorometric methods with the dyes ethidium bromide and trisphenanthroline ruthenium(II).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009049823345
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Porosified Silicon Wafer Structures Impregnated With Platinum Anti-Tumor Compounds: Fabrication, Characterization, and Diffusion Studies (2000)
    Springer
    Biomedical microdevices 2 (2000), S. 265-272 
    Details
    ISSN: 1572-8781
    Schlagwort(e): porous silicon ; cis-platin ; drug delivery ; calcium phosphate ; carbo-platin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Technik allgemein
    Notizen: Abstract In this work, the incorporation and characterization of cis-platin (cis-diammine dichloroplatinum(II)), carbo-platin [cis-diammine(cyclobutane-1,1-dicarboxylato] platinum(II)), and Pt(en)Cl2 (ethylenediamminedichloro platinum(II)) within layers of calcium phosphate on porous Si/Si substrates are described. These materials have been characterized by scanning electron microscopy, secondary ion mass spectrometry, and X-ray energy dispersive spectroscopy. The diffusion of platinum species from the doped calcium phosphate layers has also been investigated by UV-visible absorption spectrometry and inductively-coupled plasma spectroscopy. The influence of initial platinum concentration, the impact of thermal annealing of the calcium phosphate/porous Si/Si matrix, as well as the effect of varying the ligand coordination sphere of the Pt complex on its ability to be delivered to the surroundings have also been analyzed. For the case of cis-platin, it is found that increasing the concentration of platinum complex in the electrolyte during cathodic growth of calcium phosphate results in a relatively greater concentration of Pt incorporated into the calcium phosphate layers and a larger amount of Pt which subsequently can be delivered to the surrounding medium upon exposure to solvent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009951121205
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Analysis of the parsley chalcone-synthase promoter in response to different light qualities (1994)
    Springer
    Planta 193 (1994), S. 275-282 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00192541
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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