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  • Column liquid chromatography  (3)
  • Doubly labelled water  (3)
  • Fire intensity  (3)
  • Springer  (9)
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  • Springer  (9)
  • 1
    ISSN: 1432-1939
    Keywords: Key words Daily energy expenditure ; Doubly labelled water ; Parental effort ; Parus major ; Working day length
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The variation in time and energy allocation of female great tits, Parus major, was studied in five different European populations across a latitudinal gradient. Daily energy expenditure (DEE) was measured in females tending 12-day-old broods. The number of daylight hours used by the parents to collect food for the brood increased with latitude, while DEE and feeding rate per brood tended to level off with latitude. Individual variation in DEE could be explained by variation in ambient temperature (–), the duration of activity period (+) and area, but not by brood size, female body mass, brood mass or feeding rate. When the effect of ambient temperature and the duration of the activity period on the day of energy expenditure measurements were controlled for, female DEE still tended to level off with latitude. Temperature and activity alone can thus not explain the observed pattern. The present study suggests that parents at southern latitudes may be under a time constraint and do not increase energy expenditure because they have no more daylight hours available for foraging, while birds at northern latitudes may be under an energy constraint because they do not make full use of the long daylight period available.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1939
    Keywords: Fire intensity ; Adenostoma fasciculatum ; Lignotuber ; Resprouting ; Herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the population structure of the lignotuberous resprouter Adenostoma fasciculatum were investigated by experimentally burning at various intensities an old stand in southern California chaparral. Survival after fire, time of resprouting, herbivory, and survival after resprouting were monitored for more than 1 year after the fire. Greater fire intensity increased plant mortality and the size of plants that died as a consequence of the fire. Time from the fire until resprouting increased with increasing fire intensity and was related to plant size: the larger the plants the earlier they resprouted. Post-resprouting mortality also increased with fire intensity and was related to time of resprouting. Herbivory increased with fire intensity and the size of plants affected by it changed with the intensity of the fire. Fire intensity had profound direct and indirect effects on the population structure of Adenostoma fasciculatum. Plant size strongly determined the direct and indirect lethal effects of fire.
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  • 3
    ISSN: 1432-1939
    Keywords: Delichon urbica ; Doubly labelled water ; House martin ; Oeciacus hirundinis ; Parasite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We measured the energy cost of ectoparasitism in an experimental study of the house martin bug Oeciacus hirundinis and its main host, nestlings of the house martin Delichon urbica. Nests were randomly assigned to inoculation with 0 (control) 10, or 100 bugs during egg laying, and this resulted in statistically significant differences in parasite loads following fledging of nestlings. Parasite loads negatively affected house martin nestlings as estimated from their body mass at age 16 days and from mass loss estimated over 1 day late in the nestling period. Daily energy expenditure (KJ/d), average daily metabolic rate (ml CO2/g h), and mass independent daily energy expenditure (kJ/mass0.67d) did not differ significantly between experimental treatments. However, average daily metabolic rate increased with increasing intensities of ectoparasitism. Mass independent daily energy expenditure also increased with higher levels of parasite infestation. These results demonstrate that the bug imposes an energy cost on its host by elevating the level of metabolism.
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  • 4
    ISSN: 1432-1939
    Keywords: Adenostoma fasciculatum ; California chaparral ; Fire intensity ; Herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Resprouting is the main regeneration mechanism after fire in Mediterranean-type ecosystems. Herbivores play an important role in controlling postfire seedling establishment, but their influence on regeneration by resprouting is less well known. To study the effects of fire intensity on resprouting of Adenostoma fasciculatum in southern California chaparral, and its interaction with herbivory, we conducted an experimental burn at three levels of fire intensity. We found that increasing fire intensity increased plant mortality, reduced the number of resprouts per plant, and delayed the time of resprouting. Herbivory increased with fire intensity, and was related to the time of resprouting. Plants resprouting later in the season and out of synchrony with the main flush were attacked more readily by herbivores. Post-resprouting mortality also increased with fire intensity and was significantly associated with herbivory in the higher fire intensity plots. Fire intensity effects on chaparral regeneration by resprouting may be farreaching through effects on the population structure, resprout production, and growth of Adenostoma fasciculatum.
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  • 5
    ISSN: 1432-1939
    Keywords: Doubly labelled water ; Energy expenditure ; Metabolism ; Sexual selection ; Tail ornament
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Different hypotheses stress the importance of natural or sexual selection to explain the evolution and maintenance of long outermost tail feathers in the barn swallow (Hirundo rustica). Since energy costs are predicted to arise from tail length manipulation, we measured the daily energy expenditure in three experimental groups (tail-shortened, tail-elongated, and control birds) with the doubly labelled water technique. Though we did not directly measure flight cost, we assumed this to be positively related to daily energy expenditure. Mass independent daily energy expenditure (kJ/mass0.67 day) average daily metabolic rate (ml CO2/g h), and water flux (ml H2O/g day) did not show any significant difference among treatments in either sex. Males had higher values than females for the three parameters. Males with short original tail length experienced a higher water flux than originally long-tailed males. Females that laid more eggs during the breeding season or had heavier broods also showed higher levels of water flux which could imply a higher food intake. Our expectation of finding energetic costs of manipulated tail length in barn swallows with an integrated measure of metabolism was not fulfilled, and we did not find evidence for behavioural changes in the birds involved in the experiment.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 47 (1998), S. 197-202 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Non-volatile strawberry compounds ; Multivariate analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A procedure is presented for simultaneous extraction of organic acids, vitamin C and sugars from strawberries and analysis by reversed-phase (RP) HPLC and cationexchange (calcium form) chromatography. Recoveries from strawberries spiked at different levels ranged from 96 to 103 % for carbohydrates, 92 to 112 % for organic acids, and 84 % for vitamin C; the repeatability of the method evaluated as the relative standard deviation in the optimum range was 〈5 % for sugars and 7.5 % for organic acids and vitamin C. Multivariate analysis techniques, such as Bayes and SIMCA analyses, have enabled correct classification of the strawberries tested but we were unable to establish adequate models for typifying them according to their geographic origin.
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  • 7
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Amphotericin B ; Oil-water lecithin-based microemulsions ; Extraction of drug from microemulsion ; Reversed-phase HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method employing extraction then reproducible reversed-phase high-performance liquid chromatography (RPHPLC) with monitoring of the drug by absorbance at 405 nm has been developed and validated for the determination of amphotericin B in oil-water lecithin-based microemulsions. The precision and accuracy of the method are excellent (SD 2.4% and 4.2%, respectively). The established linearity range was 10–60 μg mL−1 (r 2=0.9967). The recovery of amphotericin B from spiked placebo was 〉90% over the linear range. The extraction procedure was simple and the HPLC conditions were able to separate the drug from its degradation products and excipients. The method has been used successfully for determining the amphotericin B content of microemulsions and for evaluating the chemical stability of amphotericin B-loaded microemulsions.
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  • 8
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Betablocker drugs ; Lipophilicity ; Capacity factors ; Distribution coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The reverse-phase, high-pressure liquid chromatographic capacity factors (k') of a series of 18 betablocking agents were determined in order to establish correlations with their distribution coefficient values (D), obtained from four bibliographic sources. Under the three pH conditions studied (3.5, 6.2 and 7.5) statistically significant linear correlations (p〈 0.002) between log k' and log D values were obtained for the four series studied. In this series it is concluded that the k' factor is a useful and reliable relative lipophilicity parameter.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant ecology 139 (1998), S. 91-101 
    ISSN: 1573-5052
    Keywords: Cytisus sp. ; Fire behavior ; Fire intensity ; Fire severity ; Heat output per unit area ; Mediterranean-type shrublands ; Minimum branch diameter ; Open-calorimeters ; Pyrometers ; Spain ; Temperature-residence-time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods are presented to relate temperature-residence-time at the soil surface, i.e., time above 150 ºC as an estimation of the severity of a fire, with measurements made during an experimental fire or on two post hoc measurements. The experiment was carried out in a shrubland dominated by the woody-legume Cytisus striatus subsp. eriocarpus, in Central Spain. Temperature-sensitive paints, and steam-releasing open-calorimeters were used as fire-meters during the burn. Post hoc measurements used were estimations of heat output per unit area, and measurements of the minimum diameter of branches of Cytisus remaining after the fire. Time above 150 ºC was obtained from measurements made with thermocouples placed at the soil surface in 20 contiguous 1×1 m squares of the burn plot. All other measurements were made at each 1×1 m in the 22×3 m rectangle surrounding, and including, the thermocouple squares. Various simple and multiple regression models were constructed to predict time above 150 ºC from each of the four measurements made during or after the fire. Maximum coefficients of determination obtained for regressions were 0.61 and 0.62 for water mass loss from open-calorimeters and branch diameter, respectively. Using all the variables in a multiple regression model, time above 150 ºC was related to water mass loss from open-calorimeters and heat output per unit area with a coefficient of determination of 0.77. It is concluded that estimations of time above 150 ºC at the soil surface during the passage of fire may be possible based on simple devices, such as open-calorimeters, or on biological indicators, such as minimum branch diameters. Additionally, combining two methods (open-calorimeters, estimations of heat output per unit area) may allow the reconstruction of the time above 150 ºC during the fire at a scale of 1 2, an important characteristic of a burn to understand ecosystem response to fire.
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