Summary
A procedure is presented for simultaneous extraction of organic acids, vitamin C and sugars from strawberries and analysis by reversed-phase (RP) HPLC and cationexchange (calcium form) chromatography. Recoveries from strawberries spiked at different levels ranged from 96 to 103 % for carbohydrates, 92 to 112 % for organic acids, and 84 % for vitamin C; the repeatability of the method evaluated as the relative standard deviation in the optimum range was <5 % for sugars and 7.5 % for organic acids and vitamin C. Multivariate analysis techniques, such as Bayes and SIMCA analyses, have enabled correct classification of the strawberries tested but we were unable to establish adequate models for typifying them according to their geographic origin.
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References
G. Konja, T. Lovric, Berry fruit juices, in S. Nagy, C. Chen, P. Shaw (Editors), Fruit Juice Processing Technology, Agscience Inc., Auburndale, Florida, 1993.
N. A. M. Eskin, Biochemistry of food spoilage: enzymatic browning, in Biochemistry of Foods, Academic Press, San Diego, 1990.
R. S. Kirk, R. Sawyer, Appendix 13, in Pearson's Composition and Analysis of Foods, Longman Scientific and Technical, UK, 1991.
L. W. Aurand, A. E. Woods, M. R. Wells, Carbohydrates, in Food Composition and Analysis, Van Nostrand Reinhold, New York, 1987.
C. F. Timberlake, P. Bridle, Distribution of anthocyanins in food plants, in P. Markakis (Editor) Anthocyanins as Food Colors, Academic Press, New York, 1982.
G. Skrede, R. E. Wrolstad, P. Lea, G. Enersen, J. Food Sci.57, 172 (1992).
J. Bakker, P. Bridle, S. Bellworthy, J. Sci. Food Agric.64, 31 (1994).
M. A. Romero Rodríguez, M. L. Vázquez Oderiz, J. López Hernández, J. Simal Lozano, J. Chromatogr. Sci.30, 433 (1992).
H. S. Lee, G. A. Coates, J. Micronutrient Anal.3, 199 (1987).
M. Calull, R. M. Marcé, F. Borrull, J. Chromatogr.590, 215 (1992).
C. W. Wilson, P. E. Shaw, J. Agric. Food Chem.35, 329 (1987).
M. Defernez, E. Katherine Kemsley, R. Wilson, J. Agric. Food Chem.43, 109 (1995).
K. McRae, P. Lidster, A. DeMarco, A. Dick, J. Sci. Food Agric.50, 329 (1990).
M. Forcen, A. Berna, A. Mulet, Lebensm. Wiss. Technol.26, 54 (1993).
M. Cruz, J. A. Saez, J. López, Analyst118, 801 (1993).
J. J. Mangas, R. Rodríguez, J. Moreno, D. Blanco, J. Agric. Food Chem.44, 268 (1996).
J. J. Mangas, R. Rodríguez, J. Moreno, D. Blanco, Lebensm. Wiss. Technol.29, 357 (1996).
D. Blanco, M. D. Gutiérrez, J. J. Mangas, A. Noval, Chromatographia25, 701 (1988).
M. Forina, R. Leardi, C. Armanino, S. Lanteri, PARVUS. An Extendible Package of Programs for Data Exploration, Classification and Correlation, Elsevier, Amsterdam, 1988.
S. Nikdel, S. Nagy, J. Attaway, in S. Nagy, J. Attaway, M. Rhodes (Editors) Adulteration of Fruit Juice Beverages, Marcel Dekker, New York, 1988.
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Mangas, J.J., Moreno, J., Suárez, B. et al. Liquid chromatographic analysis of non-volatile strawberry compounds. Chromatographia 47, 197–202 (1998). https://doi.org/10.1007/BF02466581
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DOI: https://doi.org/10.1007/BF02466581