ISSN:
1617-4623
Keywords:
Class II patatin gene
;
β-glucuronidase
;
Transgenic potato
;
Transgenic tobacco
;
Root tip
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A new member of the patatin gene family belonging to the class II subfamily was isolated and characterized by DNA sequencing. In order to study the expression profile of this gene, the promoter was fused to the β-glucuronidase gene and transferred to potato and tobacco. Histochemical analysis revealed high expression in a few defined cells in potato tubers and in a specific layer of both potato and tobacco root tips. In contrast to the developmentally and metabolically regulated class I patatin gene B33 this gene was not inducible by elevated levels of sucrose. Expression of this chimaeric gene was also found in callus and suspension cultures of potato.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00259611
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