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  • 1
    Electronic Resource
    Electronic Resource
    Repeated sequence RSa is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Keywords: Key wordsBradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335812
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Keywords: Bradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genome of Brahyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Brahyrhizobium strains capable of nodulating soybean was also demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335812
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Exocytosis of cortical granules from activated oocytes of the toad, Bufo arenarum (1984)
    Springer
    Cell & tissue research 237 (1984), S. 191-194 
    Details
    ISSN: 1432-0878
    Keywords: Cellular inclusions ; Cortical granules ; Exocytosis ; Oocytes ; Amphibians (Bufo arenarum)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Observation of the cortical region of oocytes of Bufo arenarum by transmission electron microscopy reveals modifications on their surface and in the contents of the cortical granules (CG) during activation. In non-activated oocytes only amorphous cortical granules (ACG) can be observed. Activated oocytes display ACG, intermediate cortical granules containing both amorphous and membranous material (ICG), and a third type containing only membranous material (MCG). During exocytosis, CG release their contents into the perivitelline space, where the amorphous and membranous materials are found. The three types of CG found during oocyte activation suggest transformation of ACG to MCG and indicate that the different components of the cortical granules, when released into the perivitelline space, might play different roles in prevention of polyspermy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229217
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    Paper (German National Licenses)
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