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  • Beta patellaris  (1)
  • Dugesia tigrina  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    A DNA library from an individual Beta patellaris chromosome conferring nematode resistance obtained by microdissection of meiotic metaphase chromosomes (1992)
    Springer
    Plant molecular biology 20 (1992), S. 503-511 
    Details
    ISSN: 1573-5028
    Keywords: sugar-beet ; Beta patellaris ; meiosis ; univalents ; microdissection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a standard protocol established for human chromosomes to create a chromosome-specific plasmid library from a Beta patellaris chromosome conferring nematode resistance. A monosomic addition line was chosen carrying 18 sugar-beet (Beta vulgaris L.) and one wild-beet (B. patellaris) chromosome. The wild-beet chromosome can readily be identified as a univalent during metaphase I of meiosis. Highly synchronized meiotic material was used to excise the univalents from four pollen mother cells. The chromatin was lysed in a 1 nl collection drop, the DNA purified and restricted with Rsa I, ligated into a vector containing universal sequencing primers, and amplified by the polymerase chain reaction. The amplified DNA was inserted into a standard plasmid vector and cloned. Approximately 23 000 recombinant plasmids were obtained of which 15 800 could be utilized. Their insert sizes ranged from 80 to 700 bp with an average of 130 bp. 61 clones were tested in more detail by genomic Southern hybridization with sugar-beet and wild-beet DNA. Of these 32 plasmids (52%) contained single-copy inserts, 11 (18%) were specific for wild-beet DNA indicating that the DNA cloned originates in the univalent chromosome. The application of this technique for establishing high-density RFLP maps for discrete regions of plant genomes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040609
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  • 2
    Electronic Resource
    Electronic Resource
    Thermal acclimation in the fresh water planarians, Dugesia tigrina and D. dorotocephala (1982)
    Springer
    Hydrobiologia 94 (1982), S. 231-236 
    Details
    ISSN: 1573-5117
    Keywords: thermal acclimation ; heat resistance ; acclimation rate ; planarians ; Dugesia tigrina ; Dugesia dorotocephala
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The planarians Dugesia tigrina and D. dorotocephala show well developed resistance adaptation responses following transfer from 5 to 25 °C or the reverse. Their rates of thermal acclimation are typical of most organisms, but the magnitudes of their responses are impressively large. Thermal acclimation in these two species is generally similar, although somewhat more rapid and perhaps more extensive in D. tigrina. Forward and reverse acclimation rates are similar and there is evidence of overshoot and undershoot time course patterns respectively. Our heat resistance data, although based on a different methodology (the CTM method, using loss of equilibrium as the end point criterion) agree well with published values for these triclads. The temperature tolerance and thermal acclimation responses of D. tigrina and D. dorotocephala correlate well with their apparent eurythermy and their widespread distributions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016404
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