ISSN:
1432-072X
Keywords:
Claviceps purpurea
;
Citrate metabolism
;
Phosphate effect
;
Glycolysis
;
Krebs cycle
;
Sugar metabolism
;
Morphological alteration
;
Alkaloid production
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In submerged cultures of Claviceps purpurea citrate utilization was stopped when the pool phosphate concentration decreased to 0.44–0.55 mmol per g of mycelial protein. The absence of citrate in the medium resulted in a 50% decrease of biomass, cessation of the alkaloid (agro-, elymo-, chanoclavine) synthesis, formation of a central large vacuole, cell enlargement and medium acidification by lactate. Citrate synthase and malate dehydrogenase gradually disappeared. Glucose-6-phosphate dehydrogenase was not detectable. The activities of glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase increased by one or two orders of magnitude higher than in control culture (with 80 mM citrate). Substrate competition on the pyruvate level is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425260
Permalink
