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  • 76  (2)
  • Bone mineral density  (2)
  • Drosophila melanogaster  (2)
  • Gene isolation  (2)
  • Holocene  (2)
  • Immunohistochemistry  (2)
  • Springer  (12)
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Keywords
Publisher
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1048-1050 
    ISSN: 1420-9071
    Keywords: Allozyme polymorphism ; linkage disequilibrium ; wine cellar and field populations ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Over three years, theAdh and α-Gpdh loci have been studied in two cellar populations ofDrosophila melanogaster and in two field populations which were each near to one of the cellars. Analyses of gene frequencies indicate that the divergence among subpopulations is greater in theAdh locus than in the α-Gpdh locus. Selection for or againstAdh S allele acting on theIn(2L)t inversion influences of the α-Gpdh alleles. This phenomenon may contribute to explain the maintenance of theAdh and α-Gpdh polymorphism and of theIn(2L)t inversion.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: E. coli trpC ; Schizophyllum TRP1 ; Complementation ; Gene isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Schizophyllum gene library was made in plasmid pRK9, Plasmids from this library were tested for their ability to complement several auxotrophic mutations of Escherichia coli. The goal was to isolate a Schizophyllum auxotrophic gene that could be used to transform a corresponding Schizophyllum auxotrophic mutant to prototrophy. Complementation was observed only for E. coli trpC indole 3-glycerol phosphate synthetase (IGPS) and phosphoribosyl-anthranilate isomerase (PRAI) mutations. Plasmids with a Schizophyllum sequence coding for both IGPS and PRAI activities were recovered from E. coli transformants. Expression of the Schizophyllum gene (TRP1) in E. coli is probably dependent on the Serratia marcescens promoter of plasmid pRK9. The DNA sequence containing the Schizophyllum TRP1 gene was not obviously rearranged in cloning.
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  • 3
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Transformation ; Gene isolation ; Basidiomycetes ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a routine way to isolate genes directly from the basidiomycete fungus, Schizophyllum commune. Plasmid DNA from a genomic gene library was used to isolate five specific genes by complementation of Schizophyllum mutations via transformation. The mutant strains were deficient in the ability to synthesize either adenine (ade2 and ade5), uracil (ural, encoding orotidine-5′-phosphate decarboxylase; OMPdecase), tryptophan (rpl, encoding indole-3-glycerol phosphate synthetase; IGPS) or para aminobenzoic acid (pab1). In each case, Southern analysis revealed that transformation to prototrophy was concomitant with the integration of vector sequence into the genome of the S. commune mutant. Total DNA from transformants was restricted, religated, and used to transform E. coli. Ampicillin resistant plasmids were recovered from E. coli and tested for their ability to transform the corresponding mutant of S. commune. Plasmids complementing the ade2, adeS, pabl, trpl, and ural mutations were recovered.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 52 (1991), S. 242-246 
    ISSN: 1432-0630
    Keywords: 75.60 ; 76
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Magnetic disaccommodation is investigated for ferrous ferrites with normal spinel structure. This study shows the presence of a new relaxation process in the temperature region IV (≃285 K) that has not been detected in inverse spinels. A numerical analysis of the isochronal spectra reveals that it is composed of two simple Debye processes with activation energies close to 0.75 eV and 0.80 eV, and preexponential factors 3×10−13 s. This relaxation is attributed to the formation of an induced anisotropy due to jumps of B-Fe ions into vacancies (as process III), but in presence of a divalent cation occupying an A-site.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 39 (1986), S. 287-289 
    ISSN: 1432-0630
    Keywords: 75.60 ; 76
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Isothermal disaccommodation curves in vacancy-doped magnetite at room temperature show a typical Richter-type behaviour. Magnetic susceptibility decreases asymptotically according to a relaxation time of about 102 s, which is analyzed in terms of a progressive stabilization of the domains walls. The relaxation strength, vacancies and the restoring pressure on the Bloch walls are closely related.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 57 (1995), S. 94-96 
    ISSN: 1432-0827
    Keywords: Androgen insensitivity syndrome ; Hormone replacement therapy ; Bone mineral density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The response of bone mass to long-term treatment with estrogen and progesterone in patients with complete androgen-insensitivity syndrome (AIS) is unknown. We report a 17-year-old female patient (karyotype 46 X, Y) with AIS studied during a 4-year period. Bone mineral density (BMD) measured by dual X-ray absorptiometry in lumbar spine and proximal femur was sharply reduced at the initial visit, and remained unchanged during long-term follow-up on hormone replacement therapy with estrogens and progestin. Bone metabolism markers were all in the normal range. The lack of significant increase in BMD highlights the importance of androgens on bone physiology that cannot be balanced in spite of an appropriate estrogenic milieu.
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  • 7
    ISSN: 1432-0827
    Keywords: Bone mineral density ; Smokers ; Premenopausal women ; Mineral metabolism ; Sex steroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Smoking is related to decreased bone mass and increased risk of osteoporotic fractures. However, the harmful effects of smoking on bone have not been well characterized. The purpose of this study was to assess the repercussions of smoking on bone mass in premenopausal women, and the relationship between these effects and parameters of mineral metabolism and hormone profile. We measured bone mineral density (BMD) in 101 premenopausal women (47 smokers, 54 nonsmokers) with dualenergy X-ray absorptiometry (DeXA) of the proximal femur and lumbar spine. In a subgroup of the sample (16 smokers, 15 nonsmokers) we measured biochemical indicators of mineral metabolism and hormone profile. BMD in the femoral neck, Ward's triangle, and the intertrochanter region was significantly lower in smoker (P〈0.05) than in nonsmokers. Concentrations of sex hormone-binding globulin were higher, and free testosterone index (FTI) was lower (P〈0.05) in smokers. We found no significant differences between the groups in parameters of mineral metabolism. Concentrations of dehydroepiandrosterone sulfate and free testosterone index were directly correlated with values of BMD in different sites. Our findings show that smoking by premenopausal women is associated with decreased BMD and characteristic changes in the hormone profile.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 278 (1994), S. 171-175 
    ISSN: 1432-0878
    Keywords: Key words: Tachykinin ; Substance P ; Sinus venosus ; Heart ; Immunohistochemistry ; Dogfish ; Scyliorhinus canicula (Elasmobranchii)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The sinus venosus of the elasmobranch heart is characterized by the presence of large bundles of unmyelinated nerve fibres that bulge into the cardiac lumen, below the endocardium. In the dogfish (Scyliorhinus canicula), these fibres contain numerous dense-core membrane-bounded granules of about 200 nm in diameter. Most intramural ganglion cells of the sinus venosus also show densely packed granules similar to those found in the subendocardial fibres. We have observed strong substance-P-like immunoreactivity in the large fibre bundles and in the perikarya of the ganglion cells. Preabsorption of the antisera with fragment 7–11 of substance P has shown that the antisera recognize the tachykinin canonic sequence. Our findings suggest that an undetermined tachykinin is secreted in the elasmobranch heart, and that it is probably released into the blood stream in the context of a little-known neuroendocrine system.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 278 (1994), S. 171-175 
    ISSN: 1432-0878
    Keywords: Tachykinin ; Substance P ; Sinus venosus ; Heart ; Immunohistochemistry ; Dogfish, Scyliorhinus canicula (Elasmobranchii)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The sinus venosus of the elasmobranch heart is characterized by the presence of large bundles of unmyelinated nerve fibres that bulge into the cardiac lumen, below the endocardium. In the dogfish (Scyliorhinus canicula), these fibres contain numerous dense-core membrane-bounded granules of about 200 nm in diameter. Most intramural ganglion cells of the sinus venosus also show densely packed granules similar to those found in the subendocardial fibres. We have observed strong substance-P-like immunoreactivity in the large fibre bundles and in the perikarya of the ganglion cells. Preabsorption of the antisera with fragment 7–11 of substance P has shown that the antisera recognize the tachykinin canonic sequence. Our findings suggest that an undetermined tachykinin is secreted in the elasmobranch heart, and that it is probably released into the blood stream in the context of a little-known neuroendocrine system.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-6278
    Keywords: Pollen analysis ; Late-glacial ; Holocene ; Iberian peninsula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Archaeology , Biology
    Notes: Abstract This paper presents the results of pollen analyses from organic sediments of seven cores (299 spectra) in a mountainous area of the north-west Iberian peninsula. The pollen diagrams, supported by seven14C dates, are used to construct a regional pollen sequence covering the main stages of vegetation dynamics, from the last phases of the Late-glacial until the present. During the Late-glacial Interstadial an important development of cryophilous forests (Betula andPinus) was recorded, although various mesophilous and thermophilous tree elements were also present. The Younger Dryas, palynologically clearly defined, is characterized by an important reduction in tree pollen percentages and the expansion of steppe formations (Poaceae andArtemisia). At the beginning of the Holocene, there was an expansion ofQuercus and a spread of other trees, which combined to give a vegetation cover of varied composition but dominated by mixed deciduous forests. Such forest formations prevailed in these mountains until 3000 years ago, when successive deforestation phases are recorded at various times as a result of increased farming activity. The results are compared with data from other mountainous areas in the northern Iberian peninsula and southern France.
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