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  • Articles  (36)
  • Column liquid chromatography  (23)
  • Beta vulgaris  (13)
  • 42.75
  • Atomic and molecular processes in external fields, including interactions with strong fields and short pulses
  • Chemistry
  • Springer  (36)
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  • Articles  (36)
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  • 1
    Electronic Resource
    Electronic Resource
    Zone electrophoretic sample treatment coupled on-line with column liquid chromatography for the determination of basic and acidic compounds in biological samples (1992)
    Springer
    Chromatographia 34 (1992), S. 581-588 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Zone-electrophoretic sample treatment ; Basic and acidic compounds ; Biological samples
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A modified valve arrangement for zone-electrophoretic sample treatment (ZEST)-which is coupled on-line with column liquid chromatography — is used to pretreat biological (plasma) samples. Carry-over of plasma proteins depends on the pH of the electrophoresis buffer. The determination of propranolol, metoprolol, cromolyn and salicylic acid demonstrates that both basic and acidic analytes can be isolated from the plasma matrix with high selectivity. Analogous piperazines, with different protein binding properties, were used to study the influence of protein binding on the recovery. It is shown that high protein can cause a decreased recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269867
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  • 2
    Electronic Resource
    Electronic Resource
    A new valve for zone-electrophoretic sample treatment coupled on-line with high performance liquid chromatography (1990)
    Springer
    Chromatographia 29 (1990), S. 217-222 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Sample treatment ; Zone electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The design of a new valve arrangement for zone-electrophoretic sample treatment (ZEST) coupled online with high performance liquid chromatography is described. Characteristics of this valve, such as the internal heat development as a function of the current, have been investigated. By using quinidine and desipramine as model compounds it is shown that charged compounds can be isolated from biological samples, in about 15 min, with high selectivity. The carry-over of proteins to the analytical column has been compared with the carry-over using a pre-column sample clean-up method. The detection limits of quinidine and hydroquinidine (50 ng/ml), using zone-electrophoretic sample treatment coupled with column liquid chromatography, are in the same range as with direct injections using pre-columns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02317907
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  • 3
    Electronic Resource
    Electronic Resource
    Electrodialytic sample treatment coupled on-line with high-performance liquid chromatography (1990)
    Springer
    Chromatographia 30 (1990), S. 361-366 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Electrodialytic sample treatment ; On-line coupling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An electrodialytic sample treatment method coupled on-line with high-performance liquid chromatography (EDIST-HPLC) is discussed in this paper. The performance of EDIST as a function of the donor-phase (sample solution) flow rate, the voltage applied over the electrodialysis block, and the time of dialysis has been studied using the basic drug ephedrine as a model compound. Enrichment of the analyte by a factor of 10–20 was possible. The determination of human plasma spiked with ephedrine is briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02328498
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  • 4
    Electronic Resource
    Electronic Resource
    Theoretical models for electrodialytic sample treatment in trace analysis by liquid chromatography (1994)
    Springer
    Chromatographia 39 (1994), S. 460-468 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Electrodialytic sample treatment ; Computer models ; Analyte enrichment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Basic considerations for analyte enrichment and recovery obtainable by electrodialysis as a sample treatment method are given. Equations are derived which describe the dependence of the concentration profiles of ionic compounds on the electric field strength in a set-up with stagnant donor and acceptor solutions. It is shown that analyte recovery increases when less ion-selective membranes are used in the electrodialysis cell. Computer models are used to estimate the analyte enrichment for a flowing donor (sample) and a stagnant acceptor phase. About 10-fold enrichment can be obtained in an electrodialytic sample treatment system within 20 min under maximum current conditions. A compromise has to be found between analyte recovery and the donor (sample) flow rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02278763
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  • 5
    Electronic Resource
    Electronic Resource
    Investigations on an interspecific hybrid involving three species of the genus Beta, with special reference to isozyme polymorphism (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 261-266 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Sugar beet ; Beta lomatogona ; Beta procumbens ; Interspecific hybrid ; Isozyme polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A tetraploid (2n=36) interspecific hybrid was obtained involving three species belonging to three different sections of Beta. The hybrid was highly sterile and did not show apomixis. At meiosis, up to nine bivalents were observed, most probably resulting from autosyndesis of the chromosomes of Beta lomatogona. For nine isozyme systems, individual enzyme expression was investigated in the parental species and in the hybrids. No silencing of genes or genomes was observed. In the case of some polymeric enzymes interspecific heteropolymers could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289283
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 11-16 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA ; Restriction fragment patterns ; Filter hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a $$\overline{\overline {COX}} II$$ gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231271
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  • 7
    Electronic Resource
    Electronic Resource
    Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of B. procumbens (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 656-664 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Beta procumbens ; Alien monosomic additions ; Plant development in vivo ; Development in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alien monosomic additions in beet (Beta vulgaris), each carrying one of the nine chromosomes of B. procumbens, were grown in vivo and in vitro to study the effect of the alien chromosomes on plant development. All additional chromosomes caused a reduction of the growth rate in vivo, which, in one case was so strong that some of the plants died as seedlings. In general, the morphological plant characteristics were not very useful to distinguish the addition types; this could have been the results of the wide variation in the recipient parent. However, some developmental characteristics proved to be highly chromosome-specific; for plants in vivo this was annuality, in combination with early or late flowering. If grown in vitro, chromosome specificity was observed for growth type (rosette or elongated stem), occurrence and rate of vitrification, occurrence and morphology of wound callus, formation of additional meristems on the midribs of leaves, formation of roots and a specific reaction to benzylaminopurine (BAP) the medium. Two chromosome types of B. procumbens caused resistance to the beet cyst nematode.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303509
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  • 8
    Electronic Resource
    Electronic Resource
    Inheritance of resistance to beet necrotic yellow vein virus in Beta vulgaris conferred by a second gene for resistance (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 740-746 
    Details
    ISSN: 1432-2242
    Keywords: Key words Beet necrotic yellow vein virus ; Beta vulgaris ; Inheritance ; Resistance genes ; Rhizomania ; STS markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Rhizomania is a serious disease of sugar beet, caused by beet necrotic yellow vein virus (BNYVV). The disease can only be controlled by the use of resistant cultivars. The accession Holly contains a single dominant gene for resistance, called Rz. The identification of a locus for resistance that differs from Rz would provide possibilities to produce cultivars with multiple resistance to BNYVV. Inheritance of resistance to BNYVV was studied by screening progenies of crosses between resistant plants of the accessions Beta vulgaris subsp. maritima WB42 and B. vulgaris subsp. vulgaris Holly-1–4 or R104. Observed and expected segregation ratios were compared to elucidate whether the resistance genes in the three accessions are alleles or situated on different loci. STS markers, linked to the genes for resistance, were used to study the segregation in more detail. The results demonstrated that the genes for resistance to BNYVV inHolly-1-4 and WB42 are closely linked. The gene for resistance in R104 is at the same locus as in Holly-1-4, and also closely linked to the gene in WB42. As the Holly resistance gene has been named Rz, the name Rz2 is proposed to refer to the resistance gene in WB42. Consequently, the gene Rz should be referred to as Rz1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051292
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  • 9
    Electronic Resource
    Electronic Resource
    Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of Beta procumbens (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 577-586 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Beta procumbens ; Alien monosomic additions ; Isozyme markers ; Chromosome identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eleven isozyme systems were used to identify the extra chromosomes, originating from Beta procumbens, in progenies of 33 monosomic additions in beet (B. vulgaris). Nine groups of monosomic additions could be distinguished, representing the nine different chromosome types of B. procumbens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260912
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  • 10
    Electronic Resource
    Electronic Resource
    Rapid hybridization-based assays for identification by DNA probes of male-sterile and male-fertile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 819-824 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA probes ; Miniassays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described whereby hybridization of mitochondrial (mt) DNA with different DNA probes can definitely distinguish male-fertile and and male-sterile (cms) cytoplasms of sugar beet Beta vulgaris L. We have developed two types of miniassays. (1) Comparative methods requiring the isolation and restriction of total cellular DNA, hybridization with cloned mtDNA fragments from either fertile or male-sterile cytoplasms, and comparison of the hybridization patterns to the fertile-and sterile-specific patterns of mtDNA of sugar beet for the given mtDNA probe. For these analyses, we routinely used 1 g of plant material to determine the type of cytoplasm. (2) Noncomparative (“plus-minus”) methods requiring neither the isolation of pure DNA nor restriction, electrophoresis, or Southern blotting. Instead, alkaline-SDS plant extracts from as little as 50 mg of plant material were dot-blotted and hybridized with fertile-specific (mitochondrial minicircular DNA) and/or cms-specific probes (consisting of a 2.3-kb mtDNA sequence exclusively occurring in the cms cytoplasm). The assays are simple to perform, give definitive results, are nonde-structive to the plants, and may be used in mass screening of sugar beet populations for hybrid production or in in vitro culture processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224996
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