Summary
A modified valve arrangement for zone-electrophoretic sample treatment (ZEST)-which is coupled on-line with column liquid chromatography — is used to pretreat biological (plasma) samples. Carry-over of plasma proteins depends on the pH of the electrophoresis buffer. The determination of propranolol, metoprolol, cromolyn and salicylic acid demonstrates that both basic and acidic analytes can be isolated from the plasma matrix with high selectivity. Analogous piperazines, with different protein binding properties, were used to study the influence of protein binding on the recovery. It is shown that high protein can cause a decreased recovery.
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Debets, A.J.J., Schoutsen, T.P., Kok, W.T. et al. Zone electrophoretic sample treatment coupled on-line with column liquid chromatography for the determination of basic and acidic compounds in biological samples. Chromatographia 34, 581–588 (1992). https://doi.org/10.1007/BF02269867
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DOI: https://doi.org/10.1007/BF02269867