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  • Affinity chromatography  (1)
  • PACS: 68.55.-a  (1)
  • Springer  (2)
  • Wiley
  • 1
    ISSN: 1432-0630
    Keywords: PACS: 68.55.-a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Fe/Al multilayers are prepared by crossed-beam pulsed laser deposition and investigated by Rutherford backscattering, conversion electron Mössbauer spectroscopy, and transmission electron microscopy. The results are compared to purely ballistic simulations of the deposition process using the TRIDYN4.0 code. It is found that the intermixing of adjacent layers must be described in terms of ballistic mixing followed by chemical mixing. The phase formation in the transition layer between adjacent layers follows the non-equilibrium behavior of Fe/Al in analogy to investigations on mechanically alloyed and ion-beam-mixed Fe/Al. In Fe-rich areas a bcc solid solution is formed. In Al-rich environment an amorphous phase is observed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Affinity chromatography ; Affinity electrophoresis ; Bile acid transport proteins ; Bile acid derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary For the purification of the bile acid transport proteins from rat hepatocytes high affinity taurocholic acid derivatives were synthesized. One type was designed for coupling to a Sepharose matrix and the other for copolymerization with acrylamide. The resulting matrices were found to be suitable for affinity chromatography and affinity electrophoresis. In both syntheses, 3α, 12α-dihydroxy-7β-amino (tauro) cholic acid derivatives were used as the starting material, since substitution in this position had previously been shown not to alter transport affinity constant.
    Type of Medium: Electronic Resource
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