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  • Articles  (5)
  • Rhizobium  (4)
  • nitrogen
  • Springer  (5)
  • Oxford University Press
  • Process Engineering, Biotechnology, Nutrition Technology  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Pure and Binary Gas Adsorption Equilibria and Kinetics of Methane and Nitrogen on 4A Zeolite by Isotope Exchange Technique (1999)
    Springer
    Adsorption 5 (1999), S. 145-158 
    Details
    ISSN: 1572-8757
    Keywords: kinetics ; isotope-exchange ; nitrogen ; adsorption ; methane ; zeolite ; equilibria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The Isotope Exchange Technique (IET) was used to simultaneously measure pure and binary gas adsorption equilibria and kinetics (self-diffusivities) of CH4 and N2 on pelletized 4A zeolite. The experiment was carried out isothermally without disturbing the adsorbed phase. CH4 was selectively adsorbed over N2 by the zeolite because of its higher polarizability. The multi-site Langmuir model described the pure gas and binary adsorption equilibria fairly well at three different temperatures. The selectivity of adsorption of CH4 over N2 increased with increasing pressure at constant gas phase composition and temperature. This curious behavior was caused by the differences in the sizes of the adsorbates. The diffusion of CH4 and N2 into the zeolite was an activated process and the Fickian diffusion model described the uptake of both pure gases and their mixtures. The self-diffusivity of N2 was an order of magnitude larger than that for CH4. The pure gas self-diffusivities for both components were constants over a large range of surface coverages (0 〈 θ 〈 0.5). The self-diffusivities of CH4 and N2 from their binary mixtures were not affected by the presence of each other, compared to their pure gas self-diffusivities at identical surface coverages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008917308002
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  • 2
    Electronic Resource
    Electronic Resource
    Discrete differences between strains of different Rhizobium spp. for competitive nodule occupancy on beans (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 681-682 
    Details
    ISSN: 1573-0972
    Keywords: Melanin pigment ; nodulation competitiveness ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Rhizobium etli strain TAL182 and R. leguminosarum bv phaseoli strain 8002, both of which produce melanin pigment, were tested for their nodulation competitiveness on beans by paired inoculation with two strains which do not produce melanin: R. tropici strain CIAT899 and Rhizobium sp. strain TAL1145. An assay was developed to distinguish nodules formed by the melanin-producing and non-producing strains. Strain TAL182 had discrete competitive superiority over CIAT899 and TAL1145 for nodulation of beans. Nodulation competitiveness was not correlated with the ability to produce melanin pigment or the host range of the Rhizobium strains tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00361016
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  • 3
    Electronic Resource
    Electronic Resource
    Polyclonal antisera production by immunization with mixed cell antigens of different rhizobial species (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 538-542 
    Details
    ISSN: 1573-0972
    Keywords: Antiserum ; Bradyrhizobium ; mixture ; polyclonal ; Rhizobium ; somatic antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Somatic antigens of Bradyrhizobium japonicum, Rhizobium sp. (Cicer arietinum) and Rhizobium sp. (Leucaena leucocephala) were prepared as standard, single-species type from cultured cells. Equal numbers of the cells of these rhizobia were then combined to obtain a mixed-rhizobial-species antigen preparation. Rabbits were immunized either with the standard, single-species type or with the mixed-rhizobial-species antigen preparations. The antisera developed from the mixed antigen immunization contained antibodies for all three rhizobial species, detectable at agglutination titres of over 800. The mixed-rhizobial-species antisera were made species specific by cross-absorption. The cross-absorbed and the mixed-rhizobial-species antisera were generally similar in quality for strain identification by agglutination, fluorescent antibodies, immunoblot and ELISA. A 66% reduction in cost was estimated for the production of antisera by immunization with mixed-rhizobial-species antigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367662
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  • 4
    Electronic Resource
    Electronic Resource
    Rhizobium plasmids in bacteria-legume interactions (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 119-125 
    Details
    ISSN: 1573-0972
    Keywords: free-living metabolism ; genomic organization ; plasmid ; Rhizobium ; symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The functional analysis of plasmids in Rhizobium strains has concentrated mainly on the symbiotic plasmid (pSym). However, genetic information relevant to both symbiotic and saprophytic Rhizobium life cycles, localized on other ‘cryptic’ replicons, has also been reported. Information is reviewed which concerns functional features encoded in plasmids other than the pSym: biosynthesis of cell surface polysaccharides, metabolic processes, the utilization of plant exudates, aromatic compounds and diverse sugars, and features involved symbiotic performance. In addition, factors which affect plasmid evolution through their influence on structural features of the plasmids, such as conjugative transfer and genomic rearrangements, is discussed. Based on the overall data, we propose that together the plasmids and the chromosome constitute a fully integrated genomic complex, entailing structural features as well as saprophytic and cellular functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364676
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L. (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 583-589 
    Details
    ISSN: 1573-0972
    Keywords: Motility ; ndv gene ; nitrogen fixation ; nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367673
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