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  • 21
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 1295-1303 
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Aluminum tolerance ; QTLs ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population. RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and 12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping. The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome 1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 267-274 
    ISSN: 1432-2242
    Keywords: Key words Tiller number ; Developmental behavior ; Quantitative trait loci (QTL) ; Rice (Oryza sativa L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A doubled-haploid rice population of 123 lines from Azucena/IR64 was used for analyzing the developmental behavior of tiller number by conditional and unconditional QTL mapping methods. It was indicated that the number of QTLs significantly affecting tiller number was different at different measuring stages. Many QTLs controlling tiller growth identified at the early stages were undetectable at the final stage. Only one QTL could be detected across the whole growth period. By conditional QTL mapping, more QTLs for tiller number could be detected than that by unconditional mapping. The temporal patterns of gene expression for tiller number could be different at different stages. Even an individual gene or genes at the same genomic region might have opposite genetic effects at various growth stages.
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  • 23
    ISSN: 1617-4623
    Keywords: Retroposon ; Colletotrichum gloeosporioides ; Anthracnose DNA fingerprinting ; Horizontal transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two genetically distinct biotypes (A and B) ofColletotrichum gloeosporioides that cause different anthracnose diseases on the legumesStylosanthes spp. have been identified in Australia. A DNA sequence that was present in biotype B and absent in biotype A was isolated by differential hybridisation of a genomic library using total genomic DNA of each biotype as hybridisation probes. This sequence also failed to hybridise to DNA of three biotypes ofC. gloeosporioides from other host species and to DNA of three other species ofColletotrichum. This clone was used to isolate two cosmid clones of biotype B. Sequence analysis of these clones revealed a repetitive element of approximately 5.7 kb in length. This element, termedCgT1, was dispersed in the genome and present in about 30 copies. The element contained open reading frames encoding deduced sequence motifs homologous togag-like proteins, reverse transcriptase and RNase H domains of non-LTR retrotransposons. The termini ofCgT1 lacked long terminal repeats (LTRs) but contained a 3′ A-rich domain. The insertion site of one copy of the element was flanked by short 13-bp direct repeats. These characteristics of the termini, taken together with the overall structure and sequence homologies, indicate thatCgT1 belongs to the non-LTR, LINE-like retrotransposon class of elements that are present in many eukaryotes. PCR primers designed to amplify regions ofCgT1 can be used to distinguish biotypes A and B in Australia. DNA fingerprinting analysis of genomic DNA using hybridisation probes derived from the terminal regions ofCgT1 revealed that Australian isolates of biotype B are monomorphic.CgT1 was not detected in some isolates causing Type B disease from other countries and whenCgT1 was present there was considerable polymorphism inCgT1 organisation in the genome.CgT1 is the first transposon-like element to be identified in the genusColletotrichum and has considerable potential as a tool for the study of population structure, genome dynamics and evolution inC. gloeosporioides.
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  • 24
    Electronic Resource
    Electronic Resource
    Springer
    Structural and multidisciplinary optimization 5 (1992), S. 37-44 
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A helicopter is intrinsically interdisciplinary due to the close coupling among aerodynamics, dynamics, and the blade structural details. Therefore a design optimization with proper interactions among appropriate disciplines (such as structure, dynamics, and aerodynamics) can offer significant benefit to improve rotor performance. This paper studies the integration of structure, dynamics, and aerodynamics in design optimization of helicopter rotor blades. The optimization is performed to minimize the rotor power required and to satisfy design requirements from structure (minimum blade weight and safe stress margin and fatigue life) and dynamics (proper placement of blade natural frequencies and free of flutter). An effort is made to formulate an effective strategy for combining these various requirements in the optimization process. The paper also presents a way for an intelligent phasing of this interdisciplinary optimization to overcome the hurdles due to conflicting demands on the design variables which arise from different disciplines.
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  • 25
    Electronic Resource
    Electronic Resource
    Springer
    Acta mechanica 134 (1999), S. 17-26 
    ISSN: 1619-6937
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary The idea of passive stabilization of a dynamical system whose motion is not asymptotically stable was advanced for the first time in the monograph [1] by introducing supplementary degrees of freedom. Based on this idea, Savchenko [2] discussed the stabilization of Hamiltonian systems by a nonlinear method (namely the method of passive stabilization by defreezing parameters). The authors of this paper investigate the effectiveness of its application to a Lagrangian system by a mechanical model which has independent scientific meaning. A comparison is also made between this model and another similar mechanical model. The problem of optimal passive stabilization is solved at the end of the paper. It is shown that this problem is closely connected with the resonance situations.
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  • 26
    Electronic Resource
    Electronic Resource
    Springer
    Rock mechanics and rock engineering 23 (1990), S. 261-273 
    ISSN: 1434-453X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: Summary First, Class II behaviour of rock is discussed with a spring model. The model is characterized by non-uniform failure, which agrees qualitatively with common experimental observation, and shows not only class I but also class II behaviour depending on strength variation of springs. Focusing on the difference between class I and class II behaviour in actual rocks, unloading-reloading tests were conducted in the post-failure region. From the test results, it was found that for both class I and class II rocks, the elastic strain tends to decrease in the post-failure region as the loadbearing capacity deteriorates. The results also show that a remarkable difference between class I and class II categories is the magnitude of non-elastic strain. That is, if non-elastic strain increases faster that elastic strain decreases, then rock shows class I behaviour, and in the opposite case class II behaviour. In general, the non-elastic strain increases with confining pressure and in some cases, rock behaviour changes from class II to class 1 at higher confining pressure.
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  • 27
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 32 (1997), S. 5661-5667 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The morphology of rubber toughened poly(methyl methacrylate) (PMMA) films was studied during deformation using environmental scanning electron microscopy (ESEM) combined with real time small angle X-ray scattering (SAXS). These two methods provide two different approaches to an in situ study of the morphology of the deforming polymer film. The ESEM study shows that the polymer film exhibits multiple craze-like streaks under tensile strain. Every streak runs through several apparently cavitated rubber particles. Further study by SAXS reveals that these streaks are not crazes. Instead they appear to be lines of cavitated rubber particles which form a particular type of dilatation band known as a “croid”. The ESEM study also shows that with increasing strain the rubber particles gradually increase in size, and that both the number and size of the croids increase. Finally the croids evolve into cracks just before the breakdown of the sample.
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  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 205 (1996), S. 121-133 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Systematically complicated technique used for preparing high-intensity (more than 8.0 GBq/cm2)241Am γ-source by a new enamel technique is presented. High intensity241Am γ-sources with activities ranging from 3.7 to 37 GBq have been made by this new technique. The activity and photon output have been measured. The results were compared with the data reported by the Radiochemical Centre Amersham in their specification. The photon output of241Am γ-source produced by us meets the technical specification of241Am γ-sources produced by Amersham. Moreover, the highest intensity can reach 1789 mCi/cm2. The overall utilization ratio of241Am activity (59.5 keV) is 31.2%.
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  • 29
    Publication Date: 2013-07-01
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 30
    Publication Date: 1994-09-27
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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