ALBERT

Description: The growing tips of plants grow sterile; therefore, disease-free plants can be generated from them. How plants safeguard growing apices from pathogen infection is still a mystery. The shoot apical meristem (SAM) is one of the three stem cells niches that give rise to the above ground plant organs. This is very well explored; however, how signaling networks orchestrate immune responses against pathogen infections in the SAM remains unclear. To reconstruct a transcriptional framework of the differentially expressed genes (DEGs) pertaining to various SAM cellular populations, we acquired large-scale transcriptome datasets from the public repository Gene Expression Omnibus (GEO). We identify here distinct sets of genes for various SAM cellular populations that are enriched in immune functions, such as immune defense, pathogen infection, biotic stress, and response to salicylic acid and jasmonic acid and their biosynthetic pathways in the SAM. We further linked those immune genes to their respective proteins and identify interactions among them by mapping a transcriptome-guided SAM-interactome. Furthermore, we compared stem-cells regulated transcriptome with innate immune responses in plants showing transcriptional separation among their DEGs in Arabidopsis. Besides unleashing a repertoire of immune-related genes in the SAM, our analysis provides a SAM-interactome that will help the community in designing functional experiments to study the specific defense dynamics of the SAM-cellular populations. Moreover, our study promotes the essence of large-scale omics data re-analysis, allowing a fresh look at the SAM-cellular transcriptome repurposing data-sets for new questions.

Description: The Liwa area is a primary food production area in the United Arab Emirates (UAE) and has intensively been used for agriculture. This study investigates the pollution levels with heavy metals in agricultural soils from the Liwa area. Thirty-two soil samples were analyzed for Mn, Zn, Cr, Ni, Cu, Pb, Cd, Co, and As. Results revealed that heavy metal levels varied in the ranges 220.02–311.21, 42.39–66.92, 43.43–71.55, 32.86–52.12, 10.29–21.70, 2.83–8.84, 0.46–0.69, 0.03–0.37 mg/kg for Mn, Zn, Cr, Ni, Cu, Pb, Cd, Co, and As, respectively. All samples presented low As concentrations with an average of 0.01 mg/kg. The variations in bulk metal contents in the soil samples were related to multiple sources, including agrochemicals, atmospheric dust containing heavy metals, and traffic-related metals. Enrichment factor analysis indicates that Cd, Ni, Zn, and Cr were highly enriched in soils, and they could originate from non-crustal sources. Based on the geo-accumulation index (Igeo), the soil samples appeared uncontaminated with Mn, Cr, Zn, Pb, Co, As, Cu, uncontaminated to moderately contaminated with Ni and moderately contaminated with Cd. The contamination factors suggest low contamination, except for Ni, which showed moderate contamination. The average pollution load index (PLI) revealed unpolluted to low pollution of all soil samples. The ecological risk assessment (PERI) showed that all heavy metals posed a low risk, except for Cd which exhibited a high ecological risk.

Description: The spatio-temporal characteristics of particulate matter with a particle size less than or equal to 2.5 μm (PM2.5), particulate matter with a particle size less than or equal to 10 μm (PM10), meteorological parameters from September 2018 to September 2019, and Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) aerosol optical depth (AOD) from 2007 to 2019 were investigated over the Central Line Project of China’s South-North Water Diversion (CSNWD) in Henan Province. To better understand the characteristics of the atmospheric environment over the CSNWD, air quality monitoring stations were installed in Nanyang (in the upper reaches), Zhengzhou (in the middle reaches), and Anyang (in the lower reaches). In this study, daily, monthly, and seasonal statistical analyses of PM2.5 and PM10 concentrations were performed and their relationship with meteorological parameters was investigated. The results show extremely poor air quality conditions over the Zhengzhou Station compared with the Nanyang and Anyang Stations. The annual average PM2.5 concentration did not meet China’s ambient air secondary standard (35 μg/m3 annual mean) over all the stations, while the annual average PM10 concentration satisfied China’s ambient air secondary standard (100 μg/m3 annual mean) over the Anyang and Nanyang Stations, except for the Zhengzhou Station. The highest PM2.5 and PM10 concentrations were observed during winter compared with the other seasons. The results show that PM2.5 and PM10 concentrations were negatively correlated with wind speed and temperature at the Nanyang and Zhengzhou Stations, but positively correlated with relative humidity. However, no significant negative or positive correlation was observed at Anyang Station. There is a strong linear positive correlation between PM2.5 and PM10 (R = 0.99), which indicates that the particulate matter at the three stations was mainly caused by local emissions. Additionally, the AOD values at the three stations were the highest in summer, which may be related to the residues of crops burned in Henan Province in summer.