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  • Organic Chemistry  (3)
  • Genetics  (1)
  • epitope  (1)
  • Wiley-Blackwell  (4)
  • Institute of Physics
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  • Wiley-Blackwell  (4)
  • Institute of Physics
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  • 1
    Digitale Medien
    Digitale Medien
    Reaktionen von 2,3-Dichlormaleinimiden mit methylenaktiven Verbindungen (1979)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 321 (1979), S. 787-796 
    Details
    ISSN: 0021-8383
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Reactions of 2,3-Dichloromaleimides with Methyleneactive Compounds2,3-Dichloromaleimides 1 react with activated methylene compounds mainly under monosubstitution to give 2-6. Replacement of the second chloroatom gives 2,3-disubstituted maleimides 9-15 and stable ylides 16-27 with pyridine and triphenylphosphine, respectively. A general synthesis for such maleimid-ylides was found in a one-batch reaction from 2,3-dichloromaleimides 1 with methyleneactive compounds and pyridine or triphenylphosphine.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prac.19793210510
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Zerfall von 1-n-Butylaminocyclohexyl-phosphonsäure-di-n-butylester (Buminafos) in wäßrigem Medium (1991)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 333 (1991), S. 85-90 
    Details
    ISSN: 0021-8383
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Degradation of n-Butylaminocyclohexyl phosphonic Acid di-n-Butylester (Buminafos) in Aqueous Medium.In aqueous medium, breakdown of Buminafos (1) is connected with total loss of its herbicidal activity. The phosphorus-carbon bond is cleaved and n-butylcyclohexylidene amine (3), cyclohexanone (5), phosphoric acid mono-n-butyl ester (4) (partly as n-butylamine salt), and 1-aminocyclohexyl phosphonic acid di-n-butylester (8) were found as degradation products of carbon-14 labeled Buminafos. Identification was performed by MS, GC/MS, 1H- and 31P-n.m.r. spectroscopy, by detection of phosphorus, and by means of the radioactivity.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prac.19913330118
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  • 3
    Digitale Medien
    Digitale Medien
    Umsetzung von 2,3-Dichlormaleinimiden mit Ethoxycarbonylmethylentriphenylphosphoran und Folgereaktionen (1979)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 321 (1979), S. 797-803 
    Details
    ISSN: 0021-8383
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Reaction of 2,3-Dichloromaleimides with Ethoxycarbonylmethylenetriphenylphosphorane and Secondary Reactions2, 3-Dichloromaleimide 1a reacts with ethoxycarbonylmethylenetriphenylphosphorane under alkenylation to give 3, 4-dichlor-5-ethoxycarbonylmethylidene-3-pyrrolin-2-one 2. With N-substituted 2, 3-Dichloromaleimides 1b-1f one chloroatom is substituted to give 2-(ethoxycarbonyltriphenylphosphoranyl)-methylene-3-chloro-maleinimides 3. The reactions of 2 and 3a with various nucleophiles are investigated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prac.19793210511
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  • 4
    Digitale Medien
    Digitale Medien
    Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1265-1274 
    Details
    ISSN: 0749-503X
    Schlagwort(e): epitope ; tag ; PCR ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Epitope tagging is the insertion of a short stretch of amino acids constituting an epitope into another protein. Tagged proteins can be identified by Western, immunoprecipitation and immunofluorescence assays using pre-existing antibodies. We have designed vectors containing the URA3 gene flanked by direct repeats of epitope tags. We use the polymerase chain reaction (PCR) to amplify the tag-URA3-tag cassette such that the ends of the PCR fragments possess homology to the gene of interest. In vivo recombination is then used to direct integration of the fragment to the location of interest, and transformants are selected by their Ura+ phenotype. Finally, selection for Ura- cells on 5-fluoro-orotic acid plates yields cells where recombination between the repeated epitopes has ‘popped out’ the URA3 gene, leaving a single copy of the epitope at the desired location. PCR epitope tagging (PET) provides a rapid and direct technique for tagging that does not require any cloning steps. We have used PET to tag three Saccharomyces cerevisiae proteins, Cln1, Sic1 and Est1.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/yea.320111306
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