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  • Gene regulation  (1)
  • Genome size Pulsed-field gel electrophoresis Cercosporin biosynthesis  (1)
  • Springer  (2)
  • Elsevier
  • Institute of Physics
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  • Springer  (2)
  • Elsevier
  • Institute of Physics
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  • 1
    Electronic Resource
    Electronic Resource
    Electrophoretic karyotype of Cercospora kikuchii (1995)
    Springer
    Current genetics 27 (1995), S. 290-292 
    Details
    ISSN: 1432-0983
    Keywords: Cercospora kikuchii ; Electrophoretic karyotype ; Genome size Pulsed-field gel electrophoresis Cercosporin biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Classical genetic analyses are not possible with the phytopathogenic fungus Cercospora kikuchii since no sexual stage has been identified. To facilitate gene mapping and to develop an understanding of the genome organization of C. kikuchii, an electrophoretic karyotype has been obtained using contour-clamped homogeneous electric field gel electrophoresis (CHEF). Eight chromosomes, two of which migrate as a doublet, have been separated into seven bands ranging from 2.0 to 5.5 Mb. Using this determination of chromosome number and size, the total genome size of C. kikuchii is estimated to be 28.4 Mb. In addition, genes encoding tubulin, ribosomal DNA, and four previously isolated light-enhanced cDNAs from C. kikuchii were assigned to chromosomes by Southern-hybridization analysis of CHEF blots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326163
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  • 2
    Electronic Resource
    Electronic Resource
    Selective DNA amplification regulates transcript levels in plant mitochondria (1995)
    Springer
    Current genetics 28 (1995), S. 113-121 
    Details
    ISSN: 1432-0983
    Keywords: Transcription ; Plant ; Mitochondria ; Copy number ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most plant mitochondrial genomes exist as subgenomic-size fragments apparently due to recombination between repetitive sequences. This leads to the possibility that independently replicating subgenomic domains could result in mitochondrial gene copy number variation. We show, through Southern-blot analysis of both restricted and intact mtDNA, that there are gene-specific copy number differences in the monocot Zea mays. Comparison of two different maize genotypes, B37(N) and B37(T), a cytoplasmic male-sterile strain, reveal fewer gene copy number differences for B37(T) than for B37(N). In contrast to maize, significant gene copy number differences are not detected in the dicot Brassica hirta. We also demonstrate that mitochondrial transcriptional rates in both species are apparently dependent on gene copy number since relative rates determined by run-on analysis are proportional to relative gene copy numbers. Thus a direct relationship exists between plant mitochondrial gene copy number and transcriptional rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315776
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