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  • Articles  (15)
  • Springer  (14)
  • Blackwell Science Ltd  (1)
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  • Articles  (15)
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  • 1
    Electronic Resource
    Electronic Resource
    Ovarian regression and apoptosis in the South American teleost Leporinus taeniatus Lütken (Characiformes, Anostomidae) from the São Francisco Basin (2005)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Journal of fish biology 67 (2005), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Involution and resorption of both postovulatory and atretic follicles were analysed in piau-jejo Leporinus taeniatus(Characiformes, Anostomidae) in order to evaluate the role of apoptosis during ovarian regression. Histological and ultrastructural analyses showed hallmarks of apoptosis in the granulosa: aggregation of compacted chromatin against the nuclear envelope, cell shrinkage, surface blebbing, loss of cell adhesion and cell fragmentation into apoptotic bodies. Protein synthesis activity preceded the onset of the cell death. The breakdown of the basement membrane led to the detachment of the granulosa cells into the follicular lumen. TUNEL-positive reactions were detected in in situ DNA fragmentation of granulosa of both postovulatory and atretic follicles. Apoptosis increased in a time-dependent manner contributing to reduction of the follicular areas. The apoptotic index (per cent of apoptotic cells) of the granulosa increased in postovulatory follicles soon after spawning, then these follicles degenerated and only remnants were observed at 7 days. In contrast, the granulosa cells reabsorbed the yolk during follicular atresia and the apoptotic index increased only in the late stage of regression. The results indicated apoptosis as the major mechanism to rapidly eliminate postovulatory follicles and being an essential process in the ovarian regression after spawning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2005.00854.x
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  • 2
    Electronic Resource
    Electronic Resource
    Posttranslational Modifications of Bone Collagen Type I are Related to the Function of Rat Femoral Regions (2000)
    Springer
    Calcified tissue international 66 (2000), S. 151-156 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Collagen — Femur — Rat — Function — Skeletal regions.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. This study analyzes the relationship between the function of femoral regions in the rat and the extent of collagen type I posttranslational modifications, to assess whether the different functional roles, i.e., mechanical or metabolic, of the bone tissues are related to the molecular structure of the matrix. For this purpose, 18 female, 100-day-old Sprague-Dawley rats were sacrificed, under anesthesia, and their femurs were removed and dissected free of adhering tissue. The spongy bone of the proximal metaphysis and the diaphysis were then selected as regions exerting prevalently a mechanical function, and the spongy bone of the distal metaphysis was selected as mainly related to metabolic function. Bone prepared from these regions was used to extract and purify the major component of the matrix, type I collagen. The content of hydroxyproline, hydroxylysine, glycosylated hydroxylysine, and pyridinium crosslinks was evaluated and the amount of each compound was expressed as a molar ratio to hydroxyproline. The amount of glycosylated hydroxylysine and pyridinium crosslinks in the distal metaphysis are significantly different from the amounts measured both in the diaphysis and the proximal metaphysis. On the contrary, the amounts of the same compounds in the diaphysis and the proximal metaphysis are statistically the same. The amount of free hydroxylysine, however, appears to be different in the proximal metaphysis and in the diaphysis. The conclusion is that matrix composition differs among different skeletal regions according to the main function they exert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002230010030
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  • 3
    Electronic Resource
    Electronic Resource
    High-temperature thermal evolution of SiAs precipitates in silicon (1992)
    Springer
    Applied physics 54 (1992), S. 221-224 
    Details
    ISSN: 1432-0630
    Keywords: 61.70 ; 64 ; 72.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The thermal evolution of monoclinic SiAs precipitates at 1050° C in silicon samples implanted with 1 and 1.5×1017 As/cm2 was followed by transmission electron microscopy (TEM) and secondary neutral mass spectrometry (SNMS). These experiments show, for the first time, the coexistence of two different states of As in silicon, i.e., the electrically active and the inactive mobile dopant, in equilibrium with monoclinic SiAs precipitates. Moreover, they provide, for the saturation concentration of As in silicon, which includes both these states, a value of 3×1021 cm−3 at 1050° C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00323840
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  • 4
    Electronic Resource
    Electronic Resource
    Alkaline phosphatase binds to collagen; a hypothesis on the mechanism of extravesicular mineralization in epiphyseal cartilage (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 836-837 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Affinity chromatography on Sepharose 4B-collagen gels was used to test the affinity of alkaline phosphatase for collagen. Results indicate that 1) alkaline phosphatase of preosseous cartilage binds to collagen probably by electrostatic interactions, 2) this interaction is inhibited by proteoglycan subunits. These results suggest that, in vivo, the formation of a collagen-alkaline phosphatase complex may be a step of the process leading to cartilage calcification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951980
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of pyridinium cross-links in human bile (1995)
    Springer
    Calcified tissue international 57 (1995), S. 415-418 
    Details
    ISSN: 1432-0827
    Keywords: Pyridinium cross-links ; Bile ; Excretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract This study evaluated whether pyridinium cross-links, which are positively charged, besides renal clearance are also cleared by the liver into bile. In 13 human bile samples tested, we were able to detect both pyridinoline (PYD) and deoxypyridinoline (DPD) in small amounts which were estimated to be about 1–2% of the amount usually found in urine. To further evaluate the amount of pyridinium cross-links excreted through bile, we studied the stability of these compounds at the alkaline pH of bile. No effect on their stability was detected over a 6-hour incubation. The origin of these molecules in bile and the significance of this finding in the use of PYD and DPD as bone resorption markers are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301942
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  • 6
    Electronic Resource
    Electronic Resource
    Collagen Type I of Rat Cortical and Trabecular Bone Differs in the Extent of Posttranslational Modifications (1996)
    Springer
    Calcified tissue international 58 (1996), S. 65-69 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Collagen — Glycosylation — Trabecular — Cortical — Bone.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. This study sought to evaluate whether the architecture of the matrix of cortical and trabecular bone is exactly the same. For this purpose we analyzed the extent of some posttranslational modifications of type I collagen, which is the major component of bone matrix. Ten female and 10 male 100-day-old rats were sacrificed and the content of hydroxylysine, glycosylated hydroxylysine, and pyridinium cross-links of collagen from cortical and trabecular bone was determined. The amount of each compound was expressed as a molar ratio with hydroxyproline. The collagen posttranslational modification pattern appears to be the same in both sexes but with a higher extent of differences in females compared with males. Comparing cortical and trabecular bone, the former contains a higher amount of hydroxylysine residues whereas in the latter, glycosylation of hydroxylysine is higher and pyridinium cross-link concentration is lower. Moreover, an inverse linear relationship between glycosylated hydroxylysine and pyridinium cross-links concentration was established, both for female (r =−0.455, P= 0.04) and male rats (r =−0.426; P= 0.06). This paper discusses what these findings may mean in functional terms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900012
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  • 7
    Electronic Resource
    Electronic Resource
    Lead-free ruthenium-based thick-film resistors: a study of model systems (1991)
    Springer
    Journal of materials science 2 (1991), S. 46-53 
    Details
    ISSN: 1573-482X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A model system of thick-film resistor (TFR) was prepared starting from RuO2 powders and a lead-free glass. The microstructural development was investigated by scanning and transmission electron microscopies, energy dispersive X-ray fluorescence, X-ray diffraction, thermogravimetry and other complementary techniques. The electrical properties of the resistors were analysed with particular attention to sheet resistance, temperature coefficient of resistance, size effects and piezoresistive properties. It was found that these simple systems are interesting with respect to their stability in ageing tests at relatively high temperatures but they are not promising for high-temperature piezoresistive gauges because of low strain sensitivity. Some samples were also prepared with a Bi2Ru2O7; a notable exchange reaction occurs between the conductive grains and the glass matrix which prevents the formation of pyrochlore-type based resistors with this glassy matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695004
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  • 8
    Electronic Resource
    Electronic Resource
    SNMS studies of ULSI gate interconnection structures (1991)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 341 (1991), S. 20-24 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Polycide gate structures are widely used in ULSI (Ultra Large Scale Integration) devices. The distribution of the dopant between the various layers is of major concern. The effects of the fluorine incorporated during WSi2 deposition have been discussed because fluorine may diffuse into the gate oxide. We have studied the redistribution of boron, phosphorus and fluorine in WSi2/poly/SiO2/poly/SiO2/Si (bulk) gate structures by using hot e-gas SNMS and nuclear analyses (RBS/NRA), in samples subjected to thermal treatments that simulate the different steps of the ULSI fabrication sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00322100
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  • 9
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    Alkaline phosphatase binds to collagen; a hypothesis on the mechanism of extravesicular mineralization in epiphyseal cartilage (1984)
    Springer
    Details
    Publication Date: 1984-08-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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  • 10
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    SNMS studies of ULSI gate interconnection structures (1991)
    Springer
    Details
    Publication Date: 1991-01-01
    Print ISSN: 0937-0633
    Electronic ISSN: 1432-1130
    Topics: Chemistry and Pharmacology
    Published by Springer
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