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  • 1
    Electronic Resource
    Electronic Resource
    Development of a genetic system for hyperthermophilic Archaea: expression of a moderate thermophilic bacterial alcohol dehydrogenase gene in Sulfolobus solfataricus (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 218 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Escherichia coli/Sulfolobus solfataricus shuttle vector pEXSs was used as a cloning vehicle for the gene transfer and expression of two bacterial genes in Sulfolobus solfataricus. The alcohol dehydrogenase (adh) from the moderate thermophilic Bacillus stearothermophilus (strain LLDR) and a mutagenised version encoding a less thermostable ADH enzyme were the selected genes. S. solfataricus adh promoter and aspartate aminotransferase terminator were used to drive the heterologous gene expression and to guarantee the correct termination of the transcripts, respectively. The constructed vectors were found to be able to carry these ‘passenger’ genes without undergoing any rearrangements. The active transcription of bacillar mRNAs was ascertained in vivo by RT-PCR. Transformed S. solfataricus expressed functional exogenous ADHs that showed unaffected kinetic and chemical–physical features.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2003.tb11506.x
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  • 2
    Electronic Resource
    Electronic Resource
    Rectified Concentrated Grape Must: HPLC Analysis of Phenolic Substances and Hydroxymethylfurfural (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The phenolic compounds extracted with ethyl acetate from 8 grape musts (5 Italian, 2 Greek, and 1 French) and from the rectified concentrated musts (RCMs) obtained from these, were analyzed. No quantifiable amount of phenolics were found by HPLC analyses of the RCMs. However, the chromatograms showed a peak that corresponded to hydroxymethylfurfural (HMF). By direct analysis of the RCMs, under the same analytical conditions used for phenolic compounds the HMF content of the RCMs was determined to range from 26 to 99 mg per kg of product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1986.tb13844.x
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  • 3
    Electronic Resource
    Electronic Resource
    CHARACTERIZATION AND CONTROL OF AN IMMOBILIZED ALLOSTERIC ENZYME SYSTEM (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 369 (1981), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1981.tb14191.x
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of an Enzymatic Capillary Membrane Reactor (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 434 (1984), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1984.tb29812.x
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  • 5
    Electronic Resource
    Electronic Resource
    Advantages in using immobilized thermophilic β-glycosidase in nonisothermal bioreactors (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 108-115 
    Details
    ISSN: 0006-3592
    Keywords: thermophilic β-glycosidase ; catalytic membranes ; nonisothermal bioreactors ; thermodialysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Catalytic membranes, obtained by immobilizing thermophilic β-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature gradients affect enzyme activity. The results showed that the thermophilic enzyme behaved as the mesophilic β-galactosidase, exhibiting an activity increase which was linearly proportional to the transmembrane temperature difference. The efficiency of the system proposed was determined by calculating two constants, α and β, which represent respectively the percentage increase of enzyme activity when a temperature difference of 1°C or a temperature gradient of 1°C cm-1 were applied across the catalytic membrane. The increase of enzyme activity in nonisothermal bioreactors entailed a proportional reduction of production times. The advantages in using thermophilic enzymes immobilized in nonisothermal bioreactors are also discussed. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:108-115, 1998.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    XPS study of laser-annealed ion-implanted GaAs (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 20 (1993), S. 955-958 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Data on the effects of 140 keV Zn+ implanation of (100)GaAs and the consequent low-power pulsed laser annealing (LPPLA) on the composition of the surface layers are presented. The implantation does was 1014 cm -2 and the sample temperature was kept at 110 ± 10°C. THE LPPLA was carried out by 10-30 pulses of a Q-switched ruby laser (λ = 694.3 nm, τ = 25 ns and P0 = 4-6 MW cm-2) equipped with a spatial homogenizer. The crystal surface was studied by XPS analysis combined with depth profiling with the use of 1 keV Ar+.The results presented include the depth redistribution of oxygen and the As/Ga ratio as well as the oxide thickness for (a) virgin, (b) as-implanted and (c) implanted and then annealed samples.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740201205
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  • 7
    Electronic Resource
    Electronic Resource
    N2O5 photolysis products investigated by fluorescence and optoacoustic techniques (1985)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 17 (1985), S. 991-1006 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Pulsed laser photolysis of N2O5 near 290 nm coupled with fluorescence detection (calibrated by NO2 photolysis) showed that the O(3P) quantum yield is ≤0.1. A pulsed laser optoacoustic technique in a flow tube (ca. 6 torr of N2) was tested by photolysis of NO2 and then applied to N2O5. Nitric oxide was added to react with NO3 free radical and the resulting increase in the optoacoustic signal confirmed the presence of NO3 free radicals. Based on the relative optoacoustic signals observed for NO2 and N2O5, the quantum yield for NO3 production is 0.8 ± 0.2.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550170906
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  • 8
    Electronic Resource
    Electronic Resource
    Homogeneous decomposition of vinyl ethers. The heat of formation of ethanal-2-yl (1979)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 11 (1979), S. 715-730 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The thermal unimolecular decomposition of three vinylethers has been studied in a VLPP apparatus. The high-pressure rate constant for the retro-ene reaction of ethylvinylether was fit by log k (sec-1) = (11.47 + 0.25) - (43.4 ± 1.0)/2.303 RT at 〈T〉 = 900 K and that of t - butylvinylether by log k (sec-1) = (12.00 ± 0.27) - (38.4 ± 1.0)/2.303 RT at 〈T〉 = 800 K. No evidence for the competition of the higher energy homolytic bond-fission process could be obtained from the experimental data. The rate constant compatible with the C—O bond scission reaction in the case of benzylvinylether was log k (sec-1) = (16.63 ± 0.30) - (53.74 ± 1.0)/2.303 RT at 〈T〉 = 750 K. Together with ΔHf,3000(benzyl·) = 47.0 kcal/mol, the activation energy for this reaction results in ΔHf,3000(CH2CHO) = +3.0 ± 2.0 kcal/mol and a corresponding resonance stabilization energy of 3.2 ± 2.0 kcal/mol for 2-ethanalyl radical.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550110705
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  • 9
    Electronic Resource
    Electronic Resource
    Laser-induced kinetics: Arrhenius parameters for t-C4H9 + XI → i-C4H9X + I (X = H,D) and the Heat of Formation of the t-butyl radical (1983)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 15 (1983), S. 1283-1300 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The metathesis reaction of DI with t-C4H9 generated by 351-nm photolysis of 2,2′-azoisopropane was studied in a low-pressure reactor (VLPφ Knudsen cell) in the temperature range of 302-411 K. The data obeyed the following Arrhenius relation when combined with recent data by Rossi and Golden gathered by the same technique (t-C4H9 by thermal decomposition of 2,2′-azoisobutane): log k2D(M-1s-1) = 9.60 - 1.90/θ, where θ = 2.303RT kcal/mol for 302 K 〈 T 〉 722 K. The metathesis reaction of HI with t-C4H9 was studied at 301 K and resulted in k2H(M-1·s-1) = (3.20 ± 0.62) × 108. An analogous Arrhenius relation was calculated for the protiated system if the small primary isotope effect k2H/k2D was assumed to be √2 at 700 K. It was of the following form: log k2H(M-1·s-1) = 9.73 - 1.68/θ.Preliminary data of Bracey and Walsh indicate that earlier Arrhenius parameters determined for the reverse reaction are somewhat in error. Their value of log k1(M-1·s-1) = 11.5 - 23.8/θ yields 7delta;Hf,3000(t-butyl) = 9.2 kcal/mol and S3000(t-butyl) = 74.2 cal/mol7°K when taken in conjuction with this study.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550151204
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  • 10
    Electronic Resource
    Electronic Resource
    The influence of reductive pretreatments on CuO/MnO2 catalytic activity: An XPS study (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 9 (1986), S. 249-250 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740090413
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