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  • N2 fixation  (2)
  • Column liquid chromatography  (1)
  • Springer  (3)
  • Blackwell Publishing Ltd
  • 1
    Digitale Medien
    Digitale Medien
    Repeated sequence RSa is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Key wordsBradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00335812
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Bradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The genome of Brahyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Brahyrhizobium strains capable of nodulating soybean was also demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00335812
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Urine clean-up method for determination of six arsenic species by LC-AAS involving microwave assisted oxidation and hydride generation (1996)
    Springer
    Chromatographia 43 (1996), S. 507-512 
    Details
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Urine clean-up ; Arsenic speciation ; Microwave assisted oxidation ; Atomic absorption spectrometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary An effective clean-up method based on the precipitation of most high molecular mass inorganic salts and organic compounds in ethanol at −15°C has been developed for the determination of arsenite, arsenate, monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (AsB) and arsenocholine (AsC) in urine. Analyte recovery was close to 100%. The six species were separated on an anionic HPLC column and determined by microwave-assisted oxidation-hydride generation-atomic absorption spectrometry. The detection limits and relative standard deviation of the whole procedure were within the 8–15 μg L−1 and 2.5–5.3% ranges, respectively, which allows application to the analysis of urine of people exposed to arsenic.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02293002
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
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