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  • 1
    Electronic Resource
    Electronic Resource
    Repeated sequence RSa is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Keywords: Key wordsBradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335812
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 15-19 
    Details
    ISSN: 1432-0789
    Keywords: Bradyrhizobium japonicum ; Bradyrhizobium elkanii ; Repeated sequence RSα ; Polymerase chain reaction detection ; DNA hybridization ; Soil inoculant ; Nodulation tests ; N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genome of Brahyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Brahyrhizobium strains capable of nodulating soybean was also demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335812
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Urine clean-up method for determination of six arsenic species by LC-AAS involving microwave assisted oxidation and hydride generation (1996)
    Springer
    Chromatographia 43 (1996), S. 507-512 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Urine clean-up ; Arsenic speciation ; Microwave assisted oxidation ; Atomic absorption spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An effective clean-up method based on the precipitation of most high molecular mass inorganic salts and organic compounds in ethanol at −15°C has been developed for the determination of arsenite, arsenate, monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (AsB) and arsenocholine (AsC) in urine. Analyte recovery was close to 100%. The six species were separated on an anionic HPLC column and determined by microwave-assisted oxidation-hydride generation-atomic absorption spectrometry. The detection limits and relative standard deviation of the whole procedure were within the 8–15 μg L−1 and 2.5–5.3% ranges, respectively, which allows application to the analysis of urine of people exposed to arsenic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293002
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    Paper (German National Licenses)
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