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  • Restriction fragment length polymorphism  (5)
  • Key words Nitrous oxide  (3)
  • Brassica  (2)
  • Springer  (9)
  • Blackwell Publishing Ltd
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Keywords
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  • Springer  (9)
  • Blackwell Publishing Ltd
Years
  • 1
    Electronic Resource
    Electronic Resource
    Effect of three contrasting onion (Allium cepa L.) production systems on nitrous oxide emissions from soil (2000)
    Springer
    Biology and fertility of soils 31 (2000), S. 334-342 
    Details
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Clover ; Herb ley ; Soil pH ; Allium cepa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  N2O emissions were measured from three contrasting onion (Allium cepa L.) production systems over an 8.5-month period. One system was established on soil where a clover sward had 3 months earlier been ploughed in (ploughed clover site). This production system followed conventional production management practices. The other two systems were established on soil where a mixed herb ley had 3 months earlier been either ploughed or rotovated. These last two production systems followed the guidelines of the International Federation of Organic Agriculture Movements (IFOAM). Cumulative N2O emissions were significantly greater from the ploughed clover site compared to the ploughed ley site (3.8 and 1.6 kg N2O-N ha–1, respectively), while cumulative N2O emissions from the ploughed ley and rotovated ley sites were not significantly different from each other. Emissions from all sites were dominated by episodes of high N2O flux activity following seedbed preparation and drilling, when soil water suction (SWS) was shown to be the rate-controlling variable. The decline in the N2O fluxes after these peak emissions followed clear exponential relationships of the form F=Ae– kt (r≥0.91), where F is the daily flux and A is the y-intercept. First-order decay constants (k) during these periods of declining N2O fluxes (corresponding to half-lives of 2.6–3.0 days) were not significantly different in magnitude from the first-order rate constants that characterised the increasing SWS. Gross differences in cumulative emissions between the clover and ley sites were attributed to the influence of differing soil pHs at the two sites on the N2O:(N2O+N2) ratio in the denitrification products. It also appeared that fertiliser applications to the clover site had both direct and indirect effects on N2O emissions by: (1) enhancing N2O emissions via potential nitrification, (2) increasing the NO3 – supply for enhanced N2O emissions via denitrification, and (3) influencing the N2O:(N2O+N2) ratio by lowering soil pH and increasing NO3 – concentrations. Onion crop yields were greater at the clover site, mainly due to the higher density of planting made possible under a conventional production philosophy. Expressing the yield on the basis of net N2O emissions, 23 t onions kg–1 N2O-N was obtained from the ploughed clover, which was double that obtained for the two systems based on the ley site. However, when the N2O emissions from the cultivation of the soils prior to the sowing of the onions was included, all three systems produced a similar yield per kilogram of N2O-N emitted, averaging 10 t kg–1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050665
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  • 2
    Electronic Resource
    Electronic Resource
    Field method to determine N2O emission from nitrification and denitrification (1998)
    Springer
    Biology and fertility of soils 28 (1998), S. 51-55 
    Details
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Nitrification ; Denitrification ; Soil cores ; Acetylene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions via the nitrification (I nit) and denitrification (I den) pathways were successfully measured with in-field incubation of soil cores in preserving jars at 0 Pa and 5–10 Pa acetylene. From the incubations, fractions of nitrification – N2O over total N2O (I nit / I tot) – and denitrification – N2O over total N2O (I den / I tot) – were obtained. Actual field emissions of N2O via nitrification (F nit) and denitrification (F den) were calculated by multiplying the fractions from the incubation technique with the daily N2O emission (F day) determined with a direct soil cover method. The approach presented here was successful for a whole range of soil moisture conditions in intensive grassland. F nit and F den followed the trends of soil ammonium and soil nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050462
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrous oxide emissions and methane oxidation by soil following cultivation of two different leguminous pastures (1999)
    Springer
    Biology and fertility of soils 30 (1999), S. 52-60 
    Details
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Methane ; Clover ; Herb ley ; Cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions and methane (CH4) consumption were quantified following cultivation of two contrasting 4-year-old pastures. A clover sward was ploughed (to 150–200 mm depth) while a mixed herb ley sward was either ploughed (to 150–200 mm depth) or rotovated (to 50 mm depth). Cumulative N2O emissions were significantly greater following ploughing of the clover sward, with 4.01 kg N2O-N ha–1 being emitted in a 48-day period. Emissions following ploughing and rotovating of the ley sward were much less and were not statistically different from each other, with 0.26 and 0.17 kg N2O-N ha–1 being measured, respectively, over a 55-day period. The large difference in cumulative N2O between the clover and ley sites is presumably due to the initially higher soil NO3 – content, greater water filled pore space and lower soil pH at the clover site. Results from a denitrification enzyme assay conducted on soils from both sites showed a strong negative relationship (r=–0.82) between soil pH and the N2O:(N2O+N2) ratio. It is suggested that further research is required to determine if control of soil pH may provide a relatively cheap mitigation option for N2O emissions from these soils. There were no significant differences in CH4 oxidation rates due to sward type or form of cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050587
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  • 4
    Electronic Resource
    Electronic Resource
    Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 784-794 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Genome evolution ; Phylogenetic tree ; Molecular taxonomy ; Restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) of nuclear DNAs have been used to explore the origin and evolution of the six cultivated Brassica species. Extensive RFLP variation was found at the species, subspecies and variety levels. Based on RFLP data from Brassica and related genera, a detailed phylogenetic tree was generated using the PAUP microcomputer program, which permits a quantitative analysis of the interrelationships among Brassica species. The results suggested that 1) B. nigra originated from one evolutionary pathway with Sinapis arvensis or a close relative as the likely progenitor, whereas B. campestris and B. oleracea came from another pathway with a possible common ancestor in wild B. oleracea or a closely related nine chromosome species; 2) the amphidiploid species B. napus and B. juncea have evolved through different combinations of the diploid morphotypes and thus polyphyletic origins may be a common mechanism for the natural occurrence of amphidiploids in Brassica; 3) the cytoplasm has played an important role in the nuclear genome evolution of amphidiploid species when the parental diploid species contain highly differentiated cytoplasms. A scheme for the origins of diploid and amphidiploid species is depicted based on evidence gathered from nuclear RFLP analysis, cpDNA RFLP analysis, cytogenetic studies and classical taxonomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265606
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  • 5
    Electronic Resource
    Electronic Resource
    Mapping loci controlling vernalization requirement and flowering time in Brassica napus (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 727-732 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Restriction fragment length polymorphism ; Vernalization ; Days-to-flowering ; Linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapeseed cultivars (Brassica napus L.) can be classified into annual and biennial groups according to their requirement for vernalization in order to induce flowering. The genetic control of these phenotypic differences is not well understood, but this information could be valuable for the design of breeding approaches to accelerate rapeseed improvement. In order to map loci controlling this variation, a doubled haploid population, derived from a cross between annual and biennial cultivars, was evaluated for vernalization requirement and days-to-flowering in a replicated field experiment using three treatments: no vernalization, 4 weeks of vernalization and 8 weeks of vernalization. A linkage map of 132 RFLP loci was used to locate loci controlling these traits. Marker segregation in one region of linkage group 9 was strongly associated with the annual/biennial growth habit in the unvernalized treatment and with days-to-flowering in all three treatments. Two other regions with smaller effects on days-to-flowering were also identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222140
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  • 6
    Electronic Resource
    Electronic Resource
    RFLP mapping of Brassica napus using doubled haploid lines (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 615-621 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Doubled haploid ; Linkage map ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The combined use of doubled haploid lines and molecular markers can provide new genetic information for use in breeding programs. An F1-derived doubled haploid (DH) population of Brassica napus obtained from a cross between an annual canola cultivar (‘Stellar’) and a biennial rapeseed (‘Major’) was used to construct a linkage map of 132 restriction fragment length polymorphism loci. The marker loci were arranged into 22 linkage groups and six pairs of linked loci covering 1016 cM. The DH map was compared to a partial map constructed with a common set of markers for an F2 population derived from the same F1 plant, and the overall maps were not significantly different. Comparisons of maps in Brassica species suggest that less recombination occurs in B. napus (n = 19) than expected from the combined map distances of the two hypothesized diploid progenitors, B. oleracea (n = 9) and B. rapa (n=10). A high percentage (32%) of segregating marker loci were duplicated in the DH map, and conserved linkage arrangements of some duplicated loci indicated possible intergenome homoeology in the amphidiploid or intragenome duplications from the diploid progenitors. Deviation from Mendelian segregation ratios (P 〈 0.05) was observed for 30% of the marker loci in the DH population and for 24% in the F2 population. Deviation towards each parent occurred at equal frequencies in both populations and marker loci that showed deviation clustered in specific linkage groups. The DH lines and molecular marker map generated for this study can be used to map loci for agronomic traits segregating in this population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222456
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  • 7
    Electronic Resource
    Electronic Resource
    Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 497-506 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Molecular taxonomy ; Genome evolution ; Phylogenetic analysis ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RFLPs were used to study genome evolution and phylogeny in Brassica and related genera. Thirtyeight accessions, including 10 accessions of B. rapa (syn. campestris), 9 cultivated types of B. oleracea, 13 nine-chromosome wild brassicas related to B. oleracea, and 6 other species in Brassica and allied genera, were examined with more then 30 random genomic DNA probes, which identified RFLPs mapping to nine different linkage groups of the B. rapa genome. Based on the RFLP data, phylogenetic trees were constructed using the PAUP microcomputer program. Within B. rapa, accessions of pak choi, narinosa, and Chinese cabbage from East Asia constituted a group distinct from turnip and wild European populations, consistent with the hypothesis that B. rapa had two centers of domestication. A wild B. rapa accession from India was positioned in the tree between European types and East Asian types, suggesting an evolutionary pathway from Europe to India, then to South China. Cultivated B. oleracea morphotypes showed monophyletic origin with wild B. oleracea or B. alboglabra as possible ancestors. Various kales constitute a highly diverse group, and represent the primitive morphotypes of cultivated B. oleracea from which cabbage, broccoli, cauliflower, etc. probably have evolved. Cauliflower was found to be closely related to broccoli, whereas cabbage was closely related to leafy kales. A great diversity existed among the 13 collections of nine-chromosome wild brassicas related to B. oleracea, representing various taxonomic states from subspecies to species. Results from these studies suggested that two basic evolutionary pathways exist for the diploid species examined. One pathway gave rise to B. fruticulosa, B. nigra, and Sinapis arvensis, with B. adpressa or a close relative as the initial ancestor. Another pathway gave rise to B. oleracea and B. rapa, with Diplotaxis erucoides or a close relative as the initial ancestor. Raphanus sativus and Eruca sativus represented intermediate types between the two lineages, and might have been derived from introgression or hybridization between species belonging to different lineages. Molecular evidence for an ascending order of chromosome numbers in the evolution of Brassica and allied genera was obtained on the basis of RFLP data and phylogenetic analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226159
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  • 8
    Electronic Resource
    Electronic Resource
    Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 593-600 
    Details
    ISSN: 1432-2242
    Keywords: Brassica rapa ; Brassica oleracea ; Molecular taxonomy ; Phylogenetic tree ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Preliminary analysis using nuclear RFLPs provided evidence that subspecies within Brassica rapa originated from two different centers. One center is in Europe, represented by turnip and turnip rape from which the oilseed sarson was derived. A second center is in South China containing a variety of Chinese vegetables of which pak choi and narinosa seem to be the most ancient forms. Based on RFLP data, the accessions of B. oleracea examined could be divided into three distinct groups, represented by thousand head kale, broccoli and cabbage. Thousand head kale and Chinese kale appear to be the primitive types. Observations of parallel variation among subspecies of both species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260914
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  • 9
    Electronic Resource
    Electronic Resource
    A linkage map ofBrassica rapa (syn.campestris) based on restriction fragment length polymorphism loci (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 296-304 
    Details
    ISSN: 1432-2242
    Keywords: Brassica rapa ; Brassica campestris ; Restriction fragment length polymorphism ; Linkage map ; Duplicated loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A detailed linkage map ofB. rapa (syn.campestris) was constructed based on segregation of 280 restriction fragment length polymorphism loci, detected by using 188 genomic DNA clones as probes on DNAs from a F2 population of Chinese cabbage ‘MichihilF’בSpring broccoli.’ These genetic markers covered 1,850 centiMorgans (cM) and defined ten linkage groups, which equals the haploid chromosome number of this species. Extensive sequence duplication was evident by the detection of two or more segregating loci with each of 69 clones (36.7% of the total). Although some duplicated loci were randomly distributed throughout the genome, many had linkage arrangements that were conserved on different linkage groups, suggesting that large chromosome fragments were present in multiple copies. However, conservation in the linkage arrangement of duplicate loci throughout entire pairs of linkage groups was not observed. Single-copy loci were often found to be located within conserved duplicated regions, and linkage distances between some loci having conserved duplicated arrangements were substantially different between the duplicated regions. Structural rearrangements, such as insertions, deletions, and inversions or combinations of these events, seemed to be related to the alternations of map distances between duplicated loci and to the dispersal of duplicated chromosome fragments. These results suggest thatB. rapa has evolved in part by duplication of chromosomes or large chromosome fragments with subsequent structural rearrangements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02190615
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