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  • Springer  (187)
  • Molecular Diversity Preservation International  (28)
  • Blackwell Publishing Ltd
  • 11
    Electronic Resource
    Electronic Resource
    Die gelben Mutanten des Rotbauchwürgers(Laniarius atrococcineus) und der Gouldamadine(Chloebia gouldiae) in biochemischer Sicht (1964)
    Springer
    Journal of ornithology 105 (1964), S. 186-189 
    Details
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Zusammenfassung Die Lipochrome der gelbbäuchigen Mutante des Rotbauchwürgers (Laniarius atrococcineus) und der gelbköpfigen Mutante der Gouldamadine(Chloebia gouldiae) werden biochemisch analysiert und mit den Lipochromen der nicht mutierten Individuen beider Arten verglichen. Es zeigt sich, daß die beiden Mutanten in ihren gelben Federn nur gelbes Carotinoid ohne Beimengung von rotem abzulagern vermögen. Die Fähigkeit der oxydativen Umwandlung gelber Nahrungscarotinoide auf fermentativem Wege in rotes Astaxanthin, das der Norm entspricht, ist ihnen verloren gegangen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01670990
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  • 12
    Electronic Resource
    Electronic Resource
    Die chemische Charakterisierung roter Lipochrome im Gefieder der Vögel (1961)
    Springer
    Journal of ornithology 102 (1961), S. 430-438 
    Details
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary After summarizing what is known about the occurence of astaxanthin and rhodoxanthin in bird feathers, the author describes his methods which resulted in the isolation of a third red lipochrome, by name of canthaxanthin, contained in the plumage ofGuara rubra and some other birds.
    Notes: Zusammenfassung Nach einem Überblick über unsere Kenntnisse vom Vorkommen der beiden bisher aus Vogelfedern bekannt gewordenen roten Lipochrome Astaxanthin und Rhodoxanthin wird über das Auffinden, die Isolierung und die Verbreitung eines weiteren, erst 1960 in Federn festgestellten roten Lipochroms, des Canthaxanthins, berichtet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01671012
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  • 13
    Electronic Resource
    Electronic Resource
    Non-linear response and electron-electron interactions in mesoscopic metal rings (1998)
    Springer
    The European physical journal 3 (1998), S. 397-405 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 73.50.Bk General theory, scattering mechanisms - 72.10.Bg General formulation of transport theory - 72.15.Rn Quantum localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: A time-dependent electric field gives rise to a stationary non-equilibrium current I (2) around a mesoscopic metal ring threaded by a magnetic flux. We show that this current, which is proportional to the intensity of the field, is closely related to the exchange part of the interaction contribution to the equilibrium persistent current, and that the corresponding non-linear conductivity directly measures the weak localization correction to the polarization. We explicitly calculate the disorder average of I (2) in the diffusive regime as function of the frequency of the electric field and the static flux piercing the ring, and suggest an experiment to test our theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100510050327
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  • 14
    Electronic Resource
    Electronic Resource
    Über den Mechanismus der anionischen Polymerisation von Maleinsäureanhydrid (1975)
    Springer
    Colloid & polymer science 253 (1975), S. 338-339 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02352137
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  • 15
    Electronic Resource
    Electronic Resource
    Lipid transport pathways in mammalian cells (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 569-579 
    Details
    ISSN: 1420-9071
    Keywords: Lipids ; membranes ; transport ; organelles ; vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A major deficit in our understanding of membrane biogenesis in eukaryotes is the definition of mechanisms by which the lipid constituents of cell membranes are transported from their sites of intracellular synthesis to the multiplicity of membranes that constitute a typical cell. A variety of approaches have been used to examine the transport of lipids to different organelles. In many cases the development of new methods has been necessary to study the problem. These methods include cytological examination of cells labeled with fluorescent lipid analogs, improved methods of subcellular fractionation, in situ enzymology that demonstrates lipid translocation by changes in lipid structure, and cell-free reconstitution with isolated organelles. Several general patterns of lipid transport have emerged but there does not appear to be a unifying mechanism by which lipids move among different organelles. Significant evidence now exists for vesicular and metabolic energy-dependent mechanisms as well as mechanisms that are clearly independent of cellular ATP content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01939695
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  • 16
    Electronic Resource
    Electronic Resource
    Kinetic analysis and simulation of glucose transport in plasma membrane vesicles of glucose-repressed and derepressedSaccharomyces cerevisiae cells (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 1018-1023 
    Details
    ISSN: 1420-9071
    Keywords: Saccharomyces cerevisiae ; growth conditions ; kinaseless mutant ; plasma membrane vesicles ; glucose transport ; kinetics and computer simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study experimental data on the kinetic parameters investigated by other authors1–5, 11 together with own data on plasma membrane vesicles, have been subjected to a computer simulation based on the equations describing facilitated diffusion. The simulation led to an ideal fit describing the above data. From this it can be concluded that glucose is transported by facilitated diffusion, and not by active transport as was postulated by Van Steveninck14, 15. The simulation method also demonstrates that the fast sampling technique used by these authors1–5,11 underestimates the fluxes. Thus, the parameters given do not contribute to the understanding of glucose transport under different metabolic conditions. The K value of plasma membrane vesicles prepared from glucose-repressed cells is around 7 mM. Derepression, particularly by galactose, causes a highly significant increase in affinity as shown by a decrease in the K value to 2 mM. The highest affinity was measured in a triple kinaseless mutant grown on glycerol with a K value of 1 mM. If seems, therefore, that the kinetic parameters derived from initial uptake rates of glucose in intact cells1–5,11 using single flux analysis, such as Eadie-Hofstee- or Lineweaver-Burk-plots, are in error.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01950152
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  • 17
    Electronic Resource
    Electronic Resource
    A geophysical survey of the De Gerlache Seamounts: preliminary results (1998)
    Springer
    Geo-marine letters 18 (1998), S. 19-25 
    Details
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The De Gerlache Seamounts actually consist of two medium-sized guyots, with summits at depths of 350–600 m. Acoustic profiler data show no significant sediment on these guyots. Alkaline basalts dredged from the summit of the eastern guyot yield K/Ar ages between 20.1±1.0 and 23.2±1.2 Ma. Basement ridges and sediment-filled troughs between the guyots are associated with the prominent gravity anomaly extending north from the Antarctic margin. This structure possibly played a role in the guyot formation, however, the question of how the De Gerlache Seamounts are related to this gravity anomaly remains uncertain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003670050047
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  • 18
    Electronic Resource
    Electronic Resource
    Pyrogallol red-vanadium complex—a new stain for electron microscopy (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 503-510 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report on the application of a pyrogallol red-vanadium complex (PR-V) for ultracytochemical staining of proteinaceous structures in animal tissues and cell cultures. This dye may be used as a general purpose stain in electron microscopy. In contrast to osmium tetroxide, the price of the material is low and no toxic vapors are produced. The PR-V complex was prepared by addition of vanadium (IV) oxide sulfate to pyrogallol red dissolved in acetate buffer (pH 5.6). The formation of the complex was indicated by a color change from purplered (λmax=520 nm) to violet (λmax=539 nm) which occurred at equimolar concentrations of the dye and the metal salt. Under these conditions PR-V was stable for several days. The mechanism of PR-V binding was checked in dot blots using different proteins as well as heparin for control. While heparin remained unstained, proteins were stained in a dose-dependent manner. Deamination of proteins with nitric oxide strongly reduced PR-V staining in dot blots as well as in cell cultures. Optimal staining results of animal cells and tissues were obtained in specimens that had been mildly fixed for at least 1 h or longer with a mixture of 0.1% glutaraldehyde and 1.0% paraformaldehyde dissolved in phosphate-buffered saline, pH 7.2, washed with acetate buffer, pH 5.6, and subsequently treated with PR-V in the presence of 50% ethanol at room temperature. Control specimens without PR-V but treated en bloc with uranyl acetate or sodium molybdate showed similar contrast but less details in the ultrastructure of the tissue. All specimens were embedded in epoxy resin and ultrathin sections were stained conventionally with uranyl and lead salt solutions. In electron micrographs, membrane-associated particles, stress fibers and filaments of the cell cortex, collagen fibrils, tight junctions and desmosomes, and other proteinaceous components were clearly visualized only in the PR-V-treated speciments. In conclusion, the ability to bind selectively and specifically to proteinaceous structures makes PR-V a versatile stain to study the localization and distribution of these structures in cells and tissues at the ultrastructural level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473313
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  • 19
    Electronic Resource
    Electronic Resource
    Pyrogallol red-vanadium complex – a new stain for electron microscopy (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 503-510 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We report on the application of a pyrogallol red-vanadium complex (PR-V) for ultracytochemical staining of proteinaceous structures in animal tissues and cell cultures. This dye may be used as a general purpose stain in electron microscopy. In contrast to osmium tetroxide, the price of the material is low and no toxic vapors are produced. The PR-V complex was prepared by addition of vanadium (IV) oxide sulfate to pyrogallol red dissolved in acetate buffer (pH 5.6). The formation of the complex was indicated by a color change from purple-red (λmax=520 nm) to violet (λmax=539 nm) which occurred at equimolar concentrations of the dye and the metal salt. Under these conditions PR-V was stable for several days. The mechanism of PR-V binding was checked in dot blots using different proteins as well as heparin for control. While heparin remained unstained, proteins were stained in a dose-dependent manner. Deamination of proteins with nitric oxide strongly reduced PR-V staining in dot blots as well as in cell cultures. Optimal staining results of animal cells and tissues were obtained in specimens that had been mildly fixed for at least 1 h or longer with a mixture of 0.1% glutaraldehyde and 1.0% paraformaldehyde dissolved in phosphate-buffered saline, pH 7.2, washed with acetate buffer, pH 5.6, and subsequently treated with PR-V in the presence of 50% ethanol at room temperature. Control specimens without PR-V but treated en bloc with uranyl acetate or sodium molybdate showed similar contrast but less details in the ultrastructure of the tissue. All specimens were embedded in epoxy resin and ultrathin sections were stained conventionally with uranyl and lead salt solutions. In electron micrographs, membrane-associated particles, stress fibers and filaments of the cell cortex, collagen fibrils, tight junctions and desmosomes, and other proteinaceous components were clearly visualized only in the PR-V-treated specimens. In conclusion, the ability to bind selectively and specifically to proteinaceous structures makes PR-V a versatile stain to study the localization and distribution of these structures in cells and tissues at the ultrastructural level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050070
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  • 20
    Electronic Resource
    Electronic Resource
    Is the surface layer from hazelnut pollen, which is precipitated by Cuprolinic blue, an effective antigen in hay-fever patients? (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 57-60 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In electron micrographs it could be shown that hazelnut (Corylus avellana) pollen grains are covered on their surface by a diffusible 10 nm thick lamellar layer. On pollen surface as well as in pollen extract this layer could be precipitated and stained by the polycationic dye Cuprolinic blue. By subsequent application of both immunogold labeling with serum from a hay-fever patient allergic to tree pollen grains and histochemical detection with Cuprolinic blue this pollen surface layer proved to be an effective antigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493421
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