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Is the surface layer from hazelnut pollen, which is precipitated by Cuprolinic blue, an effective antigen in hay-fever patients?

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Summary

In electron micrographs it could be shown that hazelnut (Corylus avellana) pollen grains are covered on their surface by a diffusible 10 nm thick lamellar layer. On pollen surface as well as in pollen extract this layer could be precipitated and stained by the polycationic dye Cuprolinic blue. By subsequent application of both immunogold labeling with serum from a hay-fever patient allergic to tree pollen grains and histochemical detection with Cuprolinic blue this pollen surface layer proved to be an effective antigen.

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Authors and Affiliations

  1. Institut für Arterioskleroseforschung an der Universität Münster, Domagkstrasse 3, D-4400, Münster, Germany

    W. Völker

  2. Kinderklinik der Universität Freiburg, Mathildestrasse 1, D-7800, Freiburg im Breisgau, Germany

    N. J. Sinclair

  3. Hautklinik der Universität Münster, D-4400, Münster, Germany

    K. J. Kalveram

  4. Lehrstuhl für Medizinische Cytobiologie der Universität Münster, D-4400, Münster, Germany

    H. Robenek

Authors
  1. W. Völker
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  2. N. J. Sinclair
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  3. K. J. Kalveram
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  4. H. Robenek
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Völker, W., Sinclair, N.J., Kalveram, K.J. et al. Is the surface layer from hazelnut pollen, which is precipitated by Cuprolinic blue, an effective antigen in hay-fever patients?. Histochemistry 84, 57–60 (1986). https://doi.org/10.1007/BF00493421

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  • DOI: https://doi.org/10.1007/BF00493421

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