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  • 1
    Electronic Resource
    Electronic Resource
    Effect of cysteine on the kinetics of electroless nickel deposition (1996)
    Springer
    Journal of applied electrochemistry 26 (1996), S. 1273-1277 
    Details
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Cysteine is found to accelerate electroless nickel (EN) deposition and hydrogen evolution simultaneously. Changes in the activation energy of the EN reaction and the electrochemical behaviour of the EN bath due to the presence of cysteine were studied. The composition of the deposit formed was determined by XPS. The results show that the activation energy of the EN reaction is decreased when cysteine is added to the bath, and also that cysteine only influences the anodic process and has no direct effect on the cathodic process. The high resolution XPS spectra show that cysteine is adsorbed in the surface layer, and S2− in the inner layer of the deposit. It is deduced that cysteine may participate in the formation of a reactive intermediate and facilitate the oxidation of H2PO 2 − , which results in the acceleration of EN plating.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249930
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  • 2
    Electronic Resource
    Electronic Resource
    Zinc nutritional status and response to lethal level of ozone exposure in rats (1979)
    Springer
    Bulletin of environmental contamination and toxicology 21 (1979), S. 206-212 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01685412
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  • 3
    Electronic Resource
    Electronic Resource
    Charged native β-cyclodextrin as a pseudostationary phase in electrokinetic chromatography (1997)
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 32-36 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Native β-cyclodextrin, deprotonated at its secondary hydroxyl groups has been studied as pseudostationary phase for electrokinetic chromatographic separation of highly hydrophobic compounds. Using this pseudostationary phase the separation of five polycyclic aromatic hydrocarbons (PAHs) is shown. The influence of the main parameters (field strength, pH, concentration of cyclodextrin, urea and organic solvent) on the separation of PAHs and migration behaviour of β-CD is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050106
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  • 4
    Electronic Resource
    Electronic Resource
    Two partial deletion mutations involving the same Alu sequence within intron 8 of the LDL receptor gene in Korean patients with familial hypercholesterolemia (1997)
    Springer
    Human genetics 〈Berlin〉 99 (1997), S. 155-163 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Twenty-eight unrelated persons heterozygous for familial hypercholesterolemia (FH) were screened to assess the frequency and nature of major structural rearrangements at the low-density lipoprotein (LDL) receptor gene in Korean FH patients. Genomic DNA was analyzed by Southern blot hybridization with probes encompassing exons 1–18 of the LDL receptor gene. Two different deletion mutations (FH29 and FH110) were detected in three FH patients (10.7%). Each of the mutations was characterized by the use of exon-specific probes and detailed restriction mapping mediated by long-PCR (polymerase chain reaction). Mutation FH29 was a 3.83-kb deletion extending from intron 6 to intron 8 and FH110 was a 5.71-kb deletion extending from intron 8 to intron 12. In FH29, the translational reading frame was preserved and the deducible result was a cysteine-rich A and B repeat truncated protein that might be unable to bind LDL but would continue to bind β-VLDL. FH110 is presumed to be a null allele, since the deletion shifts the reading frame and results in a truncated protein that terminates in exon 13. Sequence analysis revealed that both deletions have occurred between two Alu-repetitive sequences that are in the same orientation. This suggested that in these patients the deletions were caused by an unequal crossing over event following mispairing of two Alu sequences on different chromatids during meiosis. Moreover, in both deletions, the recombinations were related to an Alu sequence in intron 8 and the deletion breakpoints are found within a specific sequence, 27 bp in length. This supports the hypothesis that this region might have some intrinsic instability, and act as one of the important factors in large recombinational rearrangements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050331
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic transformation of Cymbidium orchid by particle bombardment (1999)
    Springer
    Plant cell reports 18 (1999), S. 978-984 
    Details
    ISSN: 1432-203X
    Keywords: Key words Orchid ; Particle bombardment ; PLB ; Transformation ; Key terminologyProtocorm A small storage organ formed from the germinating embryo ; possessing an apical meristem and a leaf primordium ; protocorm-like body (PLB) A somatic protocorm derived from in vitro culture of apical or axillary bud meristems ; primary PLBs PLBs induced by culturing apical meristem-tips aseptically ; secondary PLBs PLBs formed on the surface of a primary PLB in culture ; proliferate PLBs PLBs proliferating on the surface of either primary or secondary PLBs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH200) carrying the GUS-INT and NPTII genes flanked by tobacco matrix attachment regions was introduced into the meristematic cells of PLBs by particle acceleration. The transformed PLBs were proliferated and selected for kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was then induced from the kanamycin-resistant PLBs, and transgenic plantlets were produced. Both the kanamycin-resistant PLBs and regenerated shoots expressed the GUS-INT gene. The presence of the introduced gene in the transformed orchid plants was confirmed by PCR analysis, sequencing and Southern blot analysis of the PCR product. The recovered transgenic plants were established in soil and acclimatized in the greenhouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050694
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  • 6
    Electronic Resource
    Electronic Resource
    An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus) (2000)
    Springer
    Plant cell reports 19 (2000), S. 315-320 
    Details
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Populus ; Aspen ; Cottonwood ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050019
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  • 7
    Electronic Resource
    Electronic Resource
    On the failure indices of quadratic failure criteria for optimal stacking sequence design of laminated plate (1994)
    Springer
    Applied composite materials 1 (1994), S. 81-85 
    Details
    ISSN: 1573-4897
    Keywords: Failure index ; Quadratic failure criteria ; Maximum strength ; Optimal stacking sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The quadratic failure criterion, which is intended to predict fracture, may be used as an object function for optimal stacking sequence design of laminated plate. However, calculations using a symmetric laminated plate demonstrate that Tsai-Wu theory may give incorrect optimum predictions under uniaxial loading.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00567214
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  • 8
    Electronic Resource
    Electronic Resource
    Wear behaviour of aluminium matrix composite materials (1992)
    Springer
    Journal of materials science 27 (1992), S. 793-800 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Wear behaviour of aluminium matrix composites is characterized by the dry spindle wear test under various conditions (volume fractions of reinforcements, sliding distances and speeds). Wear resistance of composites is improved due to the presence of reinforcements, but no noticeable improvements are observed in the wear resistance with more than 20% addition of reinforcements. To analyse wear mechanisms, wear surfaces are examined by scanning electron microscopy (SEM). The major wear mechanisms of discontinuous metal matrix composites (MMC)s are strongly dependent on sliding speeds. Dominant mechanism is the adhesive-abrasive wear at low and intermediate sliding speeds, and melt wear at high sliding speeds. Weight loss is linearly increased with the sliding distance. The effect of reinforcements' orientations on wear behaviours is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02403898
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  • 9
    Electronic Resource
    Electronic Resource
    Wear behaviour of aluminium matrix composite materials (1992)
    Springer
    Journal of materials science 27 (1992), S. 793-800 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Wear behaviour of aluminium matrix composites is characterized by the dry spindle wear test under various conditions (volume fractions of reinforcements, sliding distances and speeds). Wear resistance of composites is improved due to the presence of reinforcements, but no noticeable improvements are observed in the wear resistance with more than 20% addition of reinforcements. To analyse wear mechanisms, wear surfaces are examined by scanning electron microscopy (SEM). The major wear mechanisms of discontinuous metal matrix composites (MMC)s are strongly dependent on sliding speeds. Dominant mechanism is the adhesive-abrasive wear at low and intermediate sliding speeds, and melt wear at high sliding speeds. Weight loss is linearly increased with the sliding distance. The effect of reinforcements' orientations on wear behaviours is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554055
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  • 10
    Electronic Resource
    Electronic Resource
    New fluorescent labels for time-resolved detection of biomolecules (1993)
    Springer
    Journal of fluorescence 3 (1993), S. 131-139 
    Details
    ISSN: 1573-4994
    Keywords: Fluorescence ; multiplex dyes ; photochemistry ; time-resolved spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract New dyes with characteristic fluorescence lifetimes have been developed for bioanalytical applications. Based upon the concept of “multiplex dyes,” we have designed rhodamine dyes with nearly identical absorption and emission spectral characteristics but different fluorescence lifetimes. Extending this principle to applications with laser diodes, new rhodamines with functional groups for covalent coupling of analytes have been developed. The new labels exhibit absortion and fluorescence beyond 600 nm and have a high quantum efficiency, even in aqueous buffer systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00862730
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