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  • tissue culture  (6)
  • Drosophila melanogaster  (5)
  • Springer  (11)
  • American Physical Society
  • BioMed Central
  • Nature Publishing Group
  • Springer Nature
Collection
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Development of behavioral competence in youngDrosophila melanogaster adults (1989)
    Springer
    Journal of insect behavior 2 (1989), S. 575-588 
    Details
    ISSN: 1572-8889
    Keywords: aging ; behavior ; central nervous system ; Drosophila melanogaster ; Diptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have monitored the ontogeny of several behaviors performed by young Drosophila melanogasteradults. Very young flies are less active than older flies and are less responsive to gravity, light, an odorant, and sucrose applied to their tarsi. In addition, very young males do not consume sucrose or perform any courtship behaviors in response to virgin females, which provide chemical and visual stimuli to courting males. The rate at which flies become maximally competent to respond to stimuli is a function of the behavior. Sensory and motor deficits are not solely responsible for young flies' inability to respond to the stimuli, which suggests that the central nervous system continues to develop after eclosion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01053355
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  • 2
    Electronic Resource
    Electronic Resource
    Description of sexual behaviors ofDrosophila rajasekari (1989)
    Springer
    Journal of chemical ecology 15 (1989), S. 1423-1432 
    Details
    ISSN: 1573-1561
    Keywords: Courtship ; pheromones ; (Z,Z)-7,11-heptacosadiene ; Drosophila rajasekari ; Drosophila melanogaster ; Diptera ; Drosophilidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The courtship behaviors and cuticular hydrocarbons ofDrosophila rajasekari are described. Sexually mature males orient, tap, follow, vibrate their abdomens, extend and vibrate their wings, and attempt copulation during courtship. They perform these behaviors in response to immature and matureD. rajasekari of both sexes, and their courtship activities are facilitated by light. The predominant cuticular hydrocarbon found in both sexes is (Z,Z)-7,11-heptacosadiene (HCD), a compound known to be used as a courtship-stimulating sex pheromone by another fruit fly,D. melanogaster. Therefore, it is not surprising thatD. melanogaster males actively court both males and females from theD. rajasekari stock. However, HCD is apparently not used byD. rajasekari as a courtship-stimulating pheromone since matureD. rajasekari males do not courtD. melanogaster females, which produce large quantities of HCD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012374
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  • 3
    Electronic Resource
    Electronic Resource
    Courtship of young males is ubiquitous inDrosophila melanogaster (1983)
    Springer
    Behavior genetics 13 (1983), S. 517-523 
    Details
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; courtship ; homosexual behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract In eightDrosophila melanogaster stocks, males which are only a few hours old stimulate courtship which is qualitatively and, in many of the stocks, quantitatively indistinguishable from the courtship elicited by virgin females. Although the sex appeal of young males and the extent to which it declines as the males become sexually mature vary somewhat from stock to stock, homosexual courtship appears to be characteristic of the species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01065927
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  • 4
    Electronic Resource
    Electronic Resource
    A structure-function analysis of NOD, a kinesin-like protein from Drosopbila melanogaster (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 145-151 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Kinesin ; Meiosis ; Mutagenesis ; Nod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analyzed a collection of 12 mutations in the Drosophila melanogaster nod locus, which encodes a kinesin-like protein involved in female meiotic chromosome segregation. The kinesin-like domain is at the N-terminus of the protein, while the C-terminal portion of the protein is unique. Four of the mutations are missense and affect highly conserved domains of the kinesin-like portion of the predicted protein, and thus demonstrate that the sequence conservation is biologically relevant. Surprisingly, two other mutations, which behave genetically as null alleles, are the result of mutations in the last exon of the nod gene. Thus, these two mutations affect the most C-terminal residues in the unique portion of the predicted protein. Based on these mutations, we suggest that this part of the protein may also be essential for wild-type function. The mutations were induced by either gamma-rays or ethyl methanesulfonate (EMS). All of the gamma-ray induced mutations were small or large chromosomal rearrangements, while all of the EMS mutations were G → A transitions. These findings are consistent with the biochemical basis of the mode of action of each mutagen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391007
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of different carbohydrate sources upon the initiation of embryogenesis from barley microspores (1994)
    Springer
    Plant cell, tissue and organ culture 36 (1994), S. 129-133 
    Details
    ISSN: 1573-5044
    Keywords: barley ; carbohydrates ; embryogenesis ; microspores ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated microspores of Hordeum vulgare L. cv. Igri were incubated in the presence of different sugars. In the presence of maltose, the optimum concentration for the development of embryoids or calluses from the microspores was 175 mM. At this concentration 0.2% of the cells developed into embryoids or calluses. Microspores cultured without a carbohydrate source died after three days' incubation. In contrast microspores incubated in the presence of sucrose, glucose or fructose died within three days. Moreover, microspores also died when incubated in the presence of a combination of 175 mM maltose with varying concentrations of either sucrose, glucose or fructose. It is concluded that incubation of microspores in the presence of sucrose, glucose or fructose results in the death of the cells via some unknown mechanism. In contrast to this, maltose can sustain development of embryoids and calluses from cultured microspores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048323
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  • 6
    Electronic Resource
    Electronic Resource
    Preliminary studies of micropropagation of Hopea odorata, a dipterocarp tree (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 193-196 
    Details
    ISSN: 1573-5044
    Keywords: Dipterocarpaceae ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plantlet development from in vitro cultures of Hopea odorato Roxb. is described. Embryos excised from seeds and cultured on Gamborg's B5 or modified Murashige and Skoog (MS) medium with benzyladenine (BA, 2.2–22.2 μM) produced axillary shoots at cotyledonary and/or stem nodes. Shoot production was greatest in germinated embryos on modified MS medium with 8.9 μM BA. Excised axillary shoots formed few buds when cultured on medium with BA and limited root development occurred on Woody Plant Medium with naphthaleneacetic acid. Nodal explants from aseptically grown plantlets sprouted axillary shoots in modified MS medium with BA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051591
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  • 7
    Electronic Resource
    Electronic Resource
    The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots (1981)
    Springer
    Plant cell, tissue and organ culture 1 (1981), S. 229-238 
    Details
    ISSN: 1573-5044
    Keywords: tissue culture ; nitrate ; ammonium ; carbohydrate ; in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultured shoots ofRosa ‘Improved Blaze’ were used to determine the effects of sucrose and inorganic nitrogen on adventitious root formation. Shoots grown in media containing high sucrose concentrations (146.07–262.93 mM) produced more and longer roots than those grown in media containing 0–87.64 mM sucrose. This response to sucrose was related to the metabolism of sucrose rather than its osmotic properties since the use of mannitol and 3-O-methyl-α-D-glucopyranoside as osmotic substitutes did not reproduce the effect on rooting. The number and length of roots increased when the shoots were grown in media with the nitrogen concentration of the Murashige-Skoog (MS) salt formulation reduced from 60 to 7.5 mM. Neither nitrate (NO 3 − ) nor ammonium (NH 4 + ) alone at any of the concentrations tested had the effect on rooting that both had together in the ratio of the MS salt formulation. When the sucrose and nitrogen concentrations were both varied, the greatest rate of root initiation occurred on shoots grown in media with a high sucrose to nitrogen concentration ratio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02318919
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  • 8
    Electronic Resource
    Electronic Resource
    Initiation of embryogenesis from cultured barley microspores: a further investigation into the toxic effects of sucrose and glucose (1994)
    Springer
    Plant cell, tissue and organ culture 37 (1994), S. 61-65 
    Details
    ISSN: 1573-5044
    Keywords: carbohydrates ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The toxic effects of sucrose and glucose upon Hordeum vulgare L. ev Igri microspore cultures were investigated. It was concluded from this study that: -microspores could be cultured in the presence of low concentrations of glucose without any deleterious effects upon cell viability, but the microspores did not form embryos or calluses. -microspores died when incubated in the presence of 40 mM glucose during the first 2 of days of incubation, but, if glucose was added after this period, cells went on to produce embryos or calluses. -the toxic effects of sucrose upon cultured microspores were irreversible after 6 h from the start of incubation. Implications of these results on underlying causes of cell death in the presence of sucrose and glucose are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048118
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  • 9
    Electronic Resource
    Electronic Resource
    In vitro propagation of cassava (Manihot esculenta Crantz) (1986)
    Springer
    Plant cell, tissue and organ culture 6 (1986), S. 221-228 
    Details
    ISSN: 1573-5044
    Keywords: Manihot esculenta ; cassava ; propagation ; in vitro propagation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is presented for the rapid in vitro propagation of cassava (Manihot esculenta Crantz). Nodal explants were induced to grow as multiple-shoot cultures on a medium containing 1.0 μM 6-benzylamino purine (BAP), supplemented with 0.25 μM α-naphthaleneacetic acid (NAA). Nodes were removed from the shoots after three weeks of growth and subcultured on fresh culture medium. An average of 7.0 nodes were produced from each explanted node after three weeks in culture. Nodal explants were transferred to a medium containing 2.5 μM indole-3-butyric acid (IBA) to improve root initiation on the developing plantlets. Plant establishment was possible upon transfer to soil. In vitro propagation offers enhanced rates of multiplication over more conventional methods of propagation. In addition, in vitro propagation facilitates the storage and international exchange of cassava germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040007
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  • 10
    Electronic Resource
    Electronic Resource
    A programmable micropropagation apparatus using cycled liquid medium (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 211-218 
    Details
    ISSN: 1573-5044
    Keywords: endogenous microorganisms ; semi-automated micropropagation ; Spathiphyllum ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An apparatus was developed that featured programmable application of liquid medium to plant cultures for micropropagation. Computer control capabilities included liquid medium introduction and medium depth within four culture vessels, medium application and removal on an assigned schedule, schedule adjustment during a culture period and medium replacement. The medium level was controlled using an accurate custom level-sensing technique consisting of thermistors and float switches. Seven-liter polycarbonate containers were modified and used as the culture vessels. Maintaining sterility was a key constraint in the development of the plant tissue culture apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041292
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