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  • Articles  (6)
  • Senescence  (4)
  • 65D32  (2)
  • Springer  (6)
  • American Institute of Physics (AIP)
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  • Articles  (6)
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  • Springer  (6)
  • American Institute of Physics (AIP)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 12 (1987), S. 291-295 
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Group II intron ; Lariat RNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In senescing strains of the filamentous ascomycete Podospora anserina, the first intron (il) of the mitochondrial gene for cytochrome-c-oxidase subunit I (CO I), a group II intron, accumulates as a circular mitochondrial plasmid (plDNA). In both juvenile and senescent wild type strain s two highly abundant transcripts were detected homologous to il and plDNA. In this report we show that these RNAs are identical molecules having different conformations: the first is a lariat, the second a corresponding linear molecule probably resulting from breakage of the branched circular form. Our findings suggest that the transcripts arise from processing of CO I pre-mRNA, including il, rather than from transcription of the excised plasmid. The significance of the lariat RNA concerning plDNA amplification via a postulated reverse transcription mechanism is discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 2 (1980), S. 181-184 
    ISSN: 1432-0983
    Keywords: Transformation ; Senescence ; Podospora anserina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the ascomycete Podospora anserina senescence through strain aging is under nucleo-cytoplasmic control and inducible in juvenile mycelia by an ‘infective principle’ transferred after cytoplasmic contact via anastomoses. A specific DNA called plasmid-like (pl) DNA, present exclusively in aging mycelia, was found to be identical with this ‘infective principle’, since it was possible to transform juvenile protoplasts to senescence by using purified p1DNA. Therefore a specific function may be attributed to this ccc DNA. Its direct involvement in a genetically programed senescence is confirmed and its development as a vector for transfer of genetic information in eukaryotes can be undertaken.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Constructive approximation 9 (1993), S. 41-58 
    ISSN: 1432-0940
    Keywords: Primary 41A55 ; 65D30 ; 65D32 ; Secondary 42C05 ; Integration rules ; Interpolatory integration rules ; Convergence ; Distribution of points ; Weak convergence ; Potential theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Suppose that, forn≥1, $$I_n [f]: = \sum\limits_{j = 1}^n {w_{jn} f(x_{jn} )} $$ is aninterpolatory integration rule of numerical integration, that is, $$I_n [f]: = \int\limits_{ - 1}^1 {P(x)dx,} degree(P)〈 n.$$ Suppose, furthermore, that, for each continuousf:[−1, 1]→R, $$\mathop {\lim }\limits_{n \to \infty } I_n [f] = \int\limits_{ - 1}^1 {f(x)dx.} $$ What can then be said about thedistribution of the points $$\{ x_{jn} \} _{1 \leqslant j \leqslant n} $$ n→∞? In all the classical examples they havearcsin distribution. More precisely, if $$\mu _n : = \frac{1}{n}\sum\limits_{j = 1}^n {\delta _{x_{jn} } } $$ is the unit measure assigning mass 1/n to each pointx jn, then, asn→∞ $$d\mu _n (x)\mathop \to \limits^* \upsilon (x)dx: = \frac{1}{\pi }(\arcsin x)'dx = \frac{{dx}}{{\pi (1 - x^2 )^{1/2} }}.$$ Surprisingly enough, this isnot the general case. We show that the set of all possible limit distributions has the form 1/2(v(x) dx+dv(x)), wherev is an arbitrary probability measure on [−1, 1]. Moreover, given any suchv, we may find rulesI n,n≥1, with positive weights, yielding the limit distribution 1/2v(x) dx+dv(x)). We also consider generalizations when the quadratures have precision other thann−1, and when we place a weight σ in our integral.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Constructive approximation 9 (1993), S. 59-82 
    ISSN: 1432-0940
    Keywords: Primary 41A55 ; 65D30 ; 65D32 ; Secondary 42C05 ; Integration rules on (−∞, ∞) ; Interpolatory integration rules ; Convergence ; Distribution of points ; Weak convergence ; Potential theory ; Gauss quadrature ; Nevai-Ullmann distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Letw be a “nice” positive weight function on (−∞, ∞), such asw(x)=exp(−⋎x⋎α) α〉1. Suppose that, forn≥1, $$I_n [f]: = \sum\limits_{j = 1}^n {w_{jn} } f(x_{jn} )$$ is aninterpolatory integration rule for the weightw: that is for polynomialsP of degree ≤n-1, $$I_n [P]: = \int\limits_{ - \infty }^\infty {P(x)w(x)dx.} $$ Moreover, suppose that the sequence of rules {I n} n=1 t8 isconvergent: $$\mathop {\lim }\limits_{n \to \infty } I_n [f] = \int\limits_{ - \infty }^\infty {f(x)w(x)dx} $$ for all continuousf:R→R satisfying suitable integrability conditions. What then can we say about thedistribution of the points {x jn} j=1 n ,n≥1? Roughly speaking, the conclusion of this paper is thathalf the points are distributed like zeros of orthogonal polynomials forw, and half may bearbitrarily distributed. Thus half the points haveNevai-Ullmann distribution of order α, and the rest are arbitrarily distributed. We also describe the possible distributions of the integration points, when the ruleI n has precision other thann-1.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 6 (1982), S. 219-222 
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Eukaryotic cloning system ; Senescence ; Long-lived mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In developing a system for molecular cloning with the Podospora anserina plasmid (p1DNA) it is necessary to find recipient strains which are resistant to p1DNA mediated senescence. Three long lived double mutants which fail to exhibit spontaneous aging were genetically and biochemically analysed. All mutants were infected with p1DNA. The mutant ca viv became irreversibly senescent and therefore was not further tested. The second mutant, gr viv showed some symptoms of aging but never died. The third strain i viv remained resistant to aging from p1DNA infection and has thus proven to be the best host strain available for molecular cloning in this system. A DNA analysis of the latter two strains revealed: 1. There is no difference from the wild strain with respect to the structure of mtDNA and the integration site of the p1DNA. 2. Of the two strains, only i viv contains free p1DNA in its mitochondria but in low amounts if compared to the wild strain. These experimental results are interpreted as follows: 1. The gr viv strain does not liberate spontaneously the p1DNA from mtDNA, but following infection is able to replicate and express this plasmid and therefore is a potential host for transformation. 2. The i viv strain liberates the mitochondrial plasmid but does not express senescence even when infected with p1DNA. Therefore, this strain is an ideal recipient for transformation provided a marker other than senescence is cloned.
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  • 6
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Rearrangements of mtDNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mapping and transcription studies have revealed that in Podospora anserina the causative agent of senescence, a mitochondrial plasmid (p1DNA), is identical with intronl of the discontinuous gene for cytochrome-c-oxidase subunit 1 (COI), which is 2 kpb from the discontinuous gene for cytochrome b (Cytb). A mitochondrial mutant (ex1) devoid of the COI, but not of the Cytb gene provides longevity. A molecular model for the onset of senescence is presented.
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