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  • 1
    Publication Date: 2015-05-30
    Description: Persistent adaptive challenges are often met with the evolution of novel physiological traits. Although there are specific examples of single genes providing new physiological functions, studies on the origin of complex organ functions are lacking. One such derived set of complex functions is found in the Lepidopteran bursa copulatrix, an organ within the female reproductive tract that digests nutrients from the male ejaculate or spermatophore. Here, we characterized bursa physiology and the evolutionary mechanisms by which it was equipped with digestive and absorptive functionality. By studying the transcriptome of the bursa and eight other tissues, we revealed a suite of highly expressed and secreted gene products providing the bursa with a combination of stomach-like traits for mechanical and enzymatic digestion of the male spermatophore. By subsequently placing these bursa genes in an evolutionary framework, we found that the vast majority of their novel digestive functions were co-opted by borrowing genes that continue to be expressed in nonreproductive tissues. However, a number of bursa-specific genes have also arisen, some of which represent unique gene families restricted to Lepidoptera and may provide novel bursa-specific functions. This pattern of promiscuous gene borrowing and relatively infrequent evolution of tissue-specific duplicates stands in contrast to studies of the evolution of novelty via single gene co-option. Our results suggest that the evolution of complex organ-level phenotypes may often be enabled (and subsequently constrained) by changes in tissue specificity that allow expression of existing genes in novel contexts, such as reproduction. The extent to which the selective pressures encountered in these novel roles require resolution via duplication and sub/neofunctionalization is likely to be determined by the need for specialized reproductive functionality. Thus, complex physiological phenotypes such as that found in the bursa offer important opportunities for understanding the relative role of pleiotropy and specialization in adaptive evolution.
    Print ISSN: 0737-4038
    Electronic ISSN: 1537-1719
    Topics: Biology
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 67 (1996), S. 2111-2112 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A transmission x-ray absorption spectroscopy cell that can be used for air-sensitive samples with in situ treatment is described. The cell is designed with a relatively small size for use with air-sensitive powdered catalyst samples that must be loaded in a glove box. Samples can be treated in situ with gas flow or vacuum and temperature control up to 500 °C. The cell is constructed of stainless steel and designed for durability as well as ease of repair. The cells are vacuum tight and equipped with beryllium windows sealed with vacuum O-ring flanges for easy loading. Each cell, with all parts excluding the windows, costs about $2700. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 3 (1991), S. 2948-2954 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The influence of both a favorable and an adverse applied axial pressure gradient on microbubble-induced skin friction reduction was examined. An 87 mm diameter, 632 mm long model equipped with a 273 mm long cylindrical force balance was employed. Experiments were carried out in a 305 mm diameter water tunnel, at free-stream speeds of 4.6, 7.6, 10.7, 13.7, and 16.8 m/sec. Air was injected at rates as high as 12×10−3 m3/sec. Measurement of the static pressure along the body with gas injection demonstrated that gas injection did not alter the pressure gradient and that the flow remained axisymmetric. Reductions in skin friction for the zero pressure gradient case agreed well with the earlier results of Deutsch and Castano [Phys. Fluids 29, 3590 (1986)]. The adverse-gradient-induced separation of the boundary layer for speeds at and above 7.6 m/sec, for air injection rates in excess of 5.0×10−3 m3/sec. The favorable gradient strongly inhibited the drag reduction mechanism [47].
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 29 (1986), S. 3590-3597 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A reduction in skin friction drag is shown when gas is introduced into the liquid turbulent boundary layer of a submerged axisymmetric body. The 89 mm diameter, 632 mm long body has a cylindrical balance 273 mm long. Free stream speeds in the 305 mm diameter tunnel are as high as 17 m/sec, giving length Reynolds number of up to 10 million. In general, skin friction reduction is shown to increase with increasing free stream speed. At high speeds, helium injection is shown to be more effective at reducing skin friction than is air injection. Maximum skin friction reduction is near 80%—a value in good agreement with the maximum value observed in the flat plate work of Madavan et al. [Phys. Fluids 27, 356 (1984)]. While maximum skin friction reduction was found at a free stream speed of 5 m/sec for the flat plate geometry, maximum skin friction reduction was at a free stream speed of 17 m/sec for the axisymmetric geometry.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 1 (1989), S. 1360-1362 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Shear stress fluctuation statistics, as measured by flush mounted hot film probes, are presented for both microbubble and polymer injection into the same zero pressure gradient turbulent boundary layer. At equivalent values of drag reduction, the moments of the probability distribution are remarkably similar. For drag reductions larger than about 40%, the deviation of the skewness and kurtosis values from their pure water values argue against simple scaling explanations of the drag reduction phenomenon.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 2 (1990), S. 2140-2146 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An array of flush-mounted hot film probes has been used to measure the local shear stress reduction as a result of microbubble injection over an axisymmetric body at the four discrete, free-stream speeds of 4.6, 10.7, 13.2, and 16.8 m/sec. Visualization of the bubble flow pattern supplement these results at intermediate free-stream speeds. At speeds of 10.7 m/sec and above, a circumferential gradient in skin friction, with skin friction reduction larger at the top than at the bottom of the model occurs at some distance downstream of injection. For these speeds, the gradient is stronger at the lower speeds and higher gas injection conditions. Higher speeds tend to drive the axial location of the gradient farther from the injection location. At speeds below 10.7 m/sec, the flow is dominated by a double vortex structure that entrains the bubbles at the bottom and sides of the model and transports them to the top. At sufficiently high gas flow rates a cavity, large enough to be observed visually, is formed just upstream of the vortices, centered near the body midline. The axial position of the cavity is roughly independent of flow speed and gas flow conditions. The transport of bubbles by the vortices, to the top of the body, is the cause of the poor skin friction reduction performance of microbubble injection at low speeds on an axisymmetric shape. Integration of the current local skin friction results gives good agreement with earlier drag balance measurements. The persistence of the drag reduction phenomenon with axial distance as well as the statistics of the shear stress fluctuations are quite similar to what was observed earlier on flat plates.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 31 (1988), S. 744-751 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Optical and photographic surveys of microbubble boundary layers are presented. The results show that the outer edge of the bubble cloud diffuses away from the wall as the bubbles are swept downstream. The plate-on-bottom orientation contains a bubble-free region near the wall that cannot be discerned for the plate-on-top configuration. Skin friction measurements made when the bubble-free region extends to y+=200 show there is no longer any Cf reduction present suggesting bubbles are not effective when they are outside the near-wall region of the boundary layer. Bubble sizes, which increase with airflow and distance from the injection section and decrease with free-stream velocity, were measured to be between 150 and 1100 μm.
    Type of Medium: Electronic Resource
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