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  • Articles  (63)
  • Springer  (54)
  • American Geophysical Union  (9)
  • American Chemical Society
  • Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics  (30)
  • Biology  (18)
  • Geosciences  (16)
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  • Articles  (63)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 374-378 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The kinetics of pentachlorophenol (PCP) degradation by a Flavobacterium sp. ATCC 39723 has been investigated. Sodium glutamate was supplied as an additional carbon source to increase the rate of cell growth and PCP degradation. A kinetic model including PCP toxicity for cell growth and PCP inhibition of its own degradation was developed. This model was also applied to 2,4-dichlorophenol (DCP) degradation by the same organism. Although PCP and DCP are degraded by different pathways, the model describes these two degradation processes very well.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 374-378 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The kinetics of pentachlorophenol (PCP) degradation by a Flavobacterium sp. ATCC 39723 has been investigated. Sodium glutamate was supplied as an additional carbon source to increase the rate of cell growth and PCP degradation. A kinetic model including PCP toxicity for cell growth and PCP inhibition of its own degradation was developed. This model was also applied to 2,4-dichlorophenol (DCP) degradation by the same organism. Although PCP and DCP are degraded by different pathways, the model describes these two degradation processes very well.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Antibody production ; Enzyme immunolocalization ; Lilium ; Phosphoglycerate mutase (cofactor independent) ; Vitronectin antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cofactor-independent phosphoglycerate mutase (PGAM-i) was isolated to homogeneity from monocotyledonous Lilium longiflorum Thunb. Two-dimensional (2D) polyacrylamide gel electrophoresis resolved three PGAM-i forms. This enzyme was originally identified by cross-reactivity to antibodies affinity-purified from 2D gels using human vitronectin (VN). Antibody produced against a denatured protein spot from a 2D gel did not recognize VN protein, but partial protein and DNA sequencing showed similarity of the former protein to maize PGAM-i. Immunoblots from roots, styles, leaves, and anthers showed the presence of PGAM-i in all tissues examined; it was isolated predominantly from the soluble cell fraction, with some present in the insoluble cell fraction. Immunoelectron microscopy demonstrated its localization in the cytoplasm and plastids in root cells near the apical meristem. In addition, immunogold labeling detected signals from the nucleus. The immunohistochemical localization of the enzyme in the nucleus, as well as in the cytosol and plastids, indicates that lily PGAM-i might have multiple functions in the cell.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Computational mechanics 26 (2000), S. 536-546 
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The meshless local Petrov–Galerkin (MLPG) method is an effective truly meshless method for solving partial differential equations using moving least squares (MLS) interpolants. It is, however, computationally expensive for some problems. A coupled MLPG/finite element (FE) method and a coupled MLPG/boundary element (BE) method are proposed in this paper to improve the solution efficiency. A procedure is developed for the coupled MLPG/FE method and the coupled MLPG/BE method so that the continuity and compatibility are preserved on the interface of the two domains where the MLPG and FE or BE methods are applied. The validity and efficiency of the MLPG/FE and MLPG/BE methods are demonstrated through a number of examples.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Computational mechanics 26 (2000), S. 166-173 
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  A novel method is proposed by coupling the element free Galerkin (EFG) and the hybrid boundary element (HBE) methods to achieve solution efficiency and accuracy for stress analysis in solids. A modified variational formulation is derived for the present coupled EFG/HBE method so that the continuity and compatibility can be preserved on the interface between the domains of EFG and HBE. The coupled EFG/HBE method has been coded in FORTRAN. The validity and efficiency of the proposed method are demonstrated through a number of example problems. It is found that the present method can take advantages of both EFG and HBE methods. The present method is very easy to implement, and very flexible for obtaining displacements and stresses of desired accuracy in solids, as the efforts for meshing the problem domain have been significantly reduced due to the use of boundary element method (BEM).
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 26 (1988), S. 207-211 
    ISSN: 1573-4927
    Keywords: DNA sequence ; human θ1-globin gene ; cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have cloned and sequenced the human θ1-globin gene. The nucleotide sequence and organization of the human θ1 gene (exons, introns, promoter, and polyadenylation signals) are similar to those reported for the orangutan θ1-globin gene. If these genes are functional, the sequences of their θ1-globin chains would differ by only one amino acid residue (at position 137).
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Structural and multidisciplinary optimization 2 (1990), S. 23-28 
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The research work presented here is based on the concept of the integration of optimization techniques and numerical analysis with the finite element method (FEM) and computer-aided design (CAD). A microcomputer aided optimum design system, MCADS, has been developed for general structures. Certain techniques to be discussed in the paper, e.g. the semi-analytical method for design sensitivity analysis, optimization analysis modelling for shape design, application oriented user interfaces and the coupling of automated optimization and user intervention have rendered MCADS pratical and versatile in applications for engineering structures. The above techniques and an application are presented in this paper.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Structural and multidisciplinary optimization 6 (1993), S. 29-37 
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract It is presented in this paper that the structural modelling of shape optimization is composed of, in general cases, four distinct processes on geometry, design, analysis and perturbation models. The relationships between these models are discussed. An integrated modelling approach based on geometric shape parameterization and automatic mesh generation is proposed. In cooperation with this modelling approach, the semi-analytic sensitivity analysis has been effectively employed. These techniques join shape optimization with FEM and CAD packages and apply it versatilely to optimum designs of general structures. The implementation and applications of the integrated modelling approach and semi-analytic sensitivity analysis to shape optimization of structures with coupling of stress and temperature fields are illustrated.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-675X
    Keywords: Antidepressants ; apoptosis ; induction ; lymphoblasts ; lymphocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have previously found that tricyclic antidepressants (TCAs) induce apoptosis in quiescent human lymphocytes. The aim of the present study was to evaluate if TCAs induce apoptosis in proliferating human lymphocytes and in established blastoid lymphocytes also. The development of conA-induced lymphoblast populations was followed by measuring the CD25 membrane expression. Three TCA compounds were run with the following concentrations: imipramine (10, 20, 30, 40, 60μ M), clomipramine (1, 10, 20, 30, 40μ M) and citalopram (40, 60, 80, 100, 180μ M). They all induced a dose-dependent apoptosis both in continuously transformed, as well as in established lymphoblasts. Preincubation of the TCA up to 48 h did not significantly increase induction of apoptosis. The three drugs tested were found to be potent inducers of apoptosis in proliferating lymphocytes. Furthermore, we found that the apoptotic populations in proliferating and in established blastoid lymphocytes were of f airly the same magnitude than in the corresponding population in TCA-incubated resting lymphocytes. In conclusion, we demonstrate that TCAs induce apoptosis in proliferating lymphocytes, as they do in quiescent lymphocytes. Furthermore, the exent of apoptosis was even more pronounced in TCA-incubated lymphoblasts compared to TCA-treated resting lymphocytes.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 15 (1996), S. 1355-1357 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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