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  • Articles  (7)
  • Nicotiana tabacum  (7)
  • Springer  (7)
  • American Chemical Society
  • American Geophysical Union
  • Springer Nature
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  • Articles  (7)
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  • Springer  (7)
  • American Chemical Society
  • American Geophysical Union
  • Springer Nature
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  • 1
    Electronic Resource
    Electronic Resource
    Micromanipulation of male and female gametes of Nicotiana tabacum: II. Preliminary attempts for in vitro fertilization and egg cell culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 657-661 
    Details
    ISSN: 1432-203X
    Keywords: Key words In vitro fertilization ; Egg cell ; Nicotiana tabacum ; Polyethylene glycol ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This research is part of an attempt to establish an in vitro fertilization system in tobacco to aid in understanding mechanisms of fertilization. Fusions of isolated male and female gametes were induced in a polyethylene glycol solution. Fusion appears similar to that in maize. One nuclear division of both an unfertilized egg cell and a synergid was induced in KM8p medium with 1 mg/l 2,4-dichlorophenoxyacetic acid in a microchamber culture; one cellular division of the egg cell was also induced in the same medium in solid-drop culture. The osmolality of suspension culture feeder cells was critical for the development of these cells. These results indicate that in vitro fertilization is possible in tobacco, which would be the first such system in dicots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050297
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  • 2
    Electronic Resource
    Electronic Resource
    Micromanipulation of male and female gametes ofNicotiana tabacum: II. Preliminary attempts for in vitro fertilization and egg cell culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 657-661 
    Details
    ISSN: 1432-203X
    Keywords: In vitro fertilization ; Egg cell ; Nicotiana tabacum ; Polyethylene glycol ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This research is part of an attempt to establish an in vitro fertilization system in tobacco to aid in understanding mechanisms of fertilization. Fusions of isolated male and female gametes were induced in a polyethylene glycol solution. Fusion appears similar to that in maize. One nuclear division of both an unfertilized egg cell and a synergid was induced in KM8p medium with 1 mg/l 2,4-dichlorophenoxyacetic acid in a microchamber culture; one cellular division of the egg cell was also induced in the same medium in solid-drop culture. The osmolality of suspension culture feeder cells was critical for the development of these cells. These results indicate that in vitro fertilization is possible in tobacco, which would be the first such system in dicots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275510
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  • 3
    Electronic Resource
    Electronic Resource
    The fusion of sperm cells and the function of male germ unit (MGU) of tobacco (Nicotiana tabacum L.) (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 171-176 
    Details
    ISSN: 1432-2145
    Keywords: Key words Fusion ; Nicotiana tabacum ; Male germ unit ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sperm cells released from in vivo-in vitro grown pollen tubes of tobacco are associated in pairs and initially enclosed by the plasma membrane of the pollen tube. When the sperm cells are placed together, using glass microinjector needles, in an enzymatic solution, up to half undergo cellular fusion with subsequent nuclear fusion. The frequency of sperm cell fusion decreases with time during the elongation of the pollen tube, suggesting that mechanisms inhibiting self-fusion of sperm cells may develop as the pollen tube elongates through the style toward the ovule. This tendency may play an important role in inhibiting fusion of the two sperm cells inside the calcium-rich synergid where the male germ unit dissociates and sperm cells are transported to their target cells - the egg and central cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050137
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  • 4
    Electronic Resource
    Electronic Resource
    Micromanipulation of male and female gametes ofNicotiana tabacum: I. Isolation of gametes (1997)
    Springer
    Plant cell reports 16 (1997), S. 555-560 
    Details
    ISSN: 1432-203X
    Keywords: Egg cell ; Nicotiana tabacum ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation of male and female gametes is a precondition for the micromanipulation of flowering plant gametes. To reflect their condition at fertilization, isolated gametes need to be physiologically mature and vigorous. Sperm cells are isolated from pollen tubes grown on cut styles using the “in vivo/in vitro” technique. Embryo sacs are isolated 2 days after anthesis using brief treatments of minimal concentrations of cell-wall-digesting enzymes on ovules of emasculated flowers. Egg cells are then mechanically separated from the embryo sac, allowing unambiguous identification of cells. Two days is usually the minimum required for the pollen tube to penetrate the ovule and effect fertilization in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142323
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  • 5
    Electronic Resource
    Electronic Resource
    Micromanipulation of male and female gametes of Nicotiana tabacum: I. Isolation of gametes (1997)
    Springer
    Plant cell reports 16 (1997), S. 555-560 
    Details
    ISSN: 1432-203X
    Keywords: Key words Egg cell ; Nicotiana tabacum ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation of male and female gametes is a precondition for the micromanipulation of flowering plant gametes. To reflect their condition at fertilization, isolated gametes need to be physiologically mature and vigorous. Sperm cells are isolated from pollen tubes grown on cut styles using the ``in vivo/in vitro'' technique. Embryo sacs are isolated 2 days after anthesis using brief treatments of minimal concentrations of cell-wall-digesting enzymes on ovules of emasculated flowers. Egg cells are then mechanically separated from the embryo sac, allowing unambiguous identification of cells. Two days is usually the minimum required for the pollen tube to penetrate the ovule and effect fertilization in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142323
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  • 6
    Electronic Resource
    Electronic Resource
    Quantitative differences in sperm cells and organelles of tobacco (Nicotiana tabacum L.) grown under differing environmental conditions (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 814-817 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic inheritance ; Environmental conditions ; Nicotiana tabacum ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In flowers grown at warm temperatures in environmental chambers and at cooler temperatures in the greenhouse, eight parameters of the sperm-cell organization of Nicotiana tabacum were examined during sperm cell maturation using serial ultrathin sectioning, transmission electron microscopy and quantitative cytology. Despite employing the same seed source, and similar soil and nutrient conditions, the surface area and volume of the cell, the nucleus and the chondriome were larger in flowers grown in growth chambers under warmer controlled conditions, whereas the number of plastids appeared to be the same, or slightly higher, in flowers grown under cooler greenhouse conditions. These results suggest that environmental conditions may influence the quantity of cytoplasmic organelles, including mitochondria and plastids, thus potentially influencing the likelihood of male cytoplasmic inheritance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224502
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  • 7
    Electronic Resource
    Electronic Resource
    Localization of myosin on sperm-cell-associated membranes of tobacco (Nicotiana tabacum L.) (1999)
    Springer
    Protoplasma 208 (1999), S. 123-128 
    Details
    ISSN: 1615-6102
    Keywords: Immunogold ; Myosin ; Nicotiana tabacum ; Pollen tube ; Scanning electron microscopy ; Sperm cell transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Actomyosin interactions are reportedly the principal mechanism for the transport of nonmotile sperm cells of flowering plants inside the pollen tube and inside the embryo sac. Myosin has been demonstrated on the generative cell (the predecessor of sperm cells), although it is unclear from previous studies whether myosin is located directly on the plasma membrane of the male germ cells or on the external plasma membrane of the pollen cell that surrounds them. Immunogold scanning electron microscopy was used to localize myosin on isolated tobacco sperm cells, with and without associated membranes. When present, the pollen tube plasma membrane surrounding the sperm cells was labeled by an antimyosin antibody, as were pollen tube cytoplasmic organelles. Negligible labeling was observed directly on the plasma membrane of the sperm cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279082
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