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  • Cellobiose/metabolism  (1)
  • Mice
  • American Association for the Advancement of Science (AAAS)  (2)
  • 1
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    Cellodextrin transport in yeast for improved biofuel production (2010)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2010-09-11
    Description: Fungal degradation of plant biomass may provide insights for improving cellulosic biofuel production. We show that the model cellulolytic fungus Neurospora crassa relies on a high-affinity cellodextrin transport system for rapid growth on cellulose. Reconstitution of the N. crassa cellodextrin transport system in Saccharomyces cerevisiae promotes efficient growth of this yeast on cellodextrins. In simultaneous saccharification and fermentation experiments, the engineered yeast strains more rapidly convert cellulose to ethanol when compared with yeast lacking this system.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Galazka, Jonathan M -- Tian, Chaoguang -- Beeson, William T -- Martinez, Bruno -- Glass, N Louise -- Cate, Jamie H D -- New York, N.Y. -- Science. 2010 Oct 1;330(6000):84-6. doi: 10.1126/science.1192838. Epub 2010 Sep 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20829451" target="_blank"〉PubMed〈/a〉
    Keywords: *Biofuels ; Biological Transport ; Biomass ; Cellobiose/metabolism ; Cellulase/metabolism ; Cellulose/*analogs & derivatives/*metabolism ; Dextrins/*metabolism ; Ethanol/metabolism ; Fermentation ; Fungal Proteins/genetics/*metabolism ; Genetic Engineering ; Kinetics ; Membrane Transport Proteins/genetics/*metabolism ; Neurospora crassa/genetics/growth & development/*metabolism ; Saccharomyces cerevisiae/genetics/growth & development/*metabolism ; Saccharomyces cerevisiae Proteins/metabolism ; beta-Glucosidase/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer (2010)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2010-09-18
    Description: Cloning mammals by means of somatic cell nuclear transfer (SCNT) is highly inefficient because of erroneous reprogramming of the donor genome. Reprogramming errors appear to arise randomly, but the nature of nonrandom, SCNT-specific errors remains elusive. We found that Xist, a noncoding RNA that inactivates one of the two X chromosomes in females, was ectopically expressed from the active X (Xa) chromosome in cloned mouse embryos of both sexes. Deletion of Xist on Xa showed normal global gene expression and resulted in about an eight- to ninefold increase in cloning efficiency. We also identified an Xist-independent mechanism that specifically down-regulated a subset of X-linked genes through somatic-type repressive histone blocks. Thus, we have identified nonrandom reprogramming errors in mouse cloning that can be altered to improve the efficiency of SCNT methods.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Inoue, Kimiko -- Kohda, Takashi -- Sugimoto, Michihiko -- Sado, Takashi -- Ogonuki, Narumi -- Matoba, Shogo -- Shiura, Hirosuke -- Ikeda, Rieko -- Mochida, Keiji -- Fujii, Takashi -- Sawai, Ken -- Otte, Arie P -- Tian, X Cindy -- Yang, Xiangzhong -- Ishino, Fumitoshi -- Abe, Kuniya -- Ogura, Atsuo -- New York, N.Y. -- Science. 2010 Oct 22;330(6003):496-9. doi: 10.1126/science.1194174. Epub 2010 Sep 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉BioResource Center, RIKEN, 305-0024 Tsukuba, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20847234" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cloning, Organism/*methods ; Down-Regulation ; Embryo, Mammalian/metabolism ; Female ; Gene Deletion ; Gene Expression Profiling ; Male ; Mice ; *Nuclear Transfer Techniques ; RNA, Long Noncoding ; RNA, Untranslated/biosynthesis/genetics/*physiology ; *X Chromosome
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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    PAPER CURRENT
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