ISSN:
1432-0878
Keywords:
Paraventricular nucleus
;
Neurotubules
;
Axonal flow
;
Colchicine
;
Autoradiography and electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The axonal flow of neurosecretory elementary granules has been studied in the paraventricular neurons of the rat (PVN), with the help of three techniques: light microscopy, radioautography after labelling with 35S-L-cysteine, and electron microscopy. Colchicine treatment does not alter the uptake of 35S cysteine in the PVN but the flow of labelled neurosecretory material towards the neurohypophysis is interrupted. Interruption of the axonal flow is also evidenced by the stagnation of neurosecretory granules at the periphery of the neuronal cytoplasm and by the presence of numerous axonal swellings, heavily loaded with neurosecretory granules and often containing abnormal elongated granules, surrounded by a single membrane, oriented more or less parallely to the long axis of the axons. Other cell organelles and neurotubules are not altered. The present experiments bring further evidence of the arrest by colchicine of the axonal flow of secretory granules without apparent changes of the neurotubules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00306998
Permalink
