Summary
In adult rats, dehydration produced by the injection of Furosemid (Lasix®) induces the formation of new centrioles in the endothelial cells of the posterior lobe of the hypophysis.
Colchicine considerably amplifies this phenomenon and centriologenesis and cilia formation are strikingly apparent in the pituicytes. In control animals, centrioles are quite exceptional in pituicytes and endothelial cells. All steps of the assembly of centrioles from a granular matrix, and the differentiation of atypical (mainly 9+0 and 8+1) cilia are observed in pituicytes. Dehydration increases the mitotic activity of the pituicytes. The respective roles of dehydration, Furosemid and colchicine in the stimulation of centriologenesis are briefly discussed.
This work was supported by a grant (1973–1974) from the Belgian National Fund for Scientific Research and by grant no. 1.120 from the Belgian National Fund for Medical Research.
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The authors wish to thank Mrs. A.-M. Hunninck-Couck for her devoted and skillful technical assistance and Mrs. D. Libert-Kaça for kindly typing the manuscript. They are indebted to Dr. J. C. Heuson for kindly supplying the rats, and to the firm Hoechst Belgium S.A. for supplying the Furosemid.
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Hubert, JP., Flament-Durand, J. & Dustin, P. Centrioles and cilia multiplication in the pituitary of the rat after furosemid and colchicine treatment. Cell Tissue Res. 149, 349–361 (1974). https://doi.org/10.1007/BF00226769
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DOI: https://doi.org/10.1007/BF00226769