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  • Springer  (2)
  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 40 (1970), S. 59-62 
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung In der Gattung Trifolium ist sowohl nach Selbstung als auch nach Artkreuzungen häufig Inkompatibilität zu beobachten. Es wird über Untersuchungen berichtet, durch Wärmebehandlung während der Blüte die Inkompatibilität zu überwinden. Zu diesen Zweck wurden die Blüten der mütterlichen Eltern einer Temperatur von 40 °C ausgesetzt und danach mit Pollen von unbehandelten Pflanzen bestäubt, die auf dem Feld oder im Gewächshaus aufblühten: Trifolium pratense wurde mit T. ambiguum, T. hirtum, T. ochroleucum, T. rubens, T. sarosiense und T. medium bestäubt, T. medium mit T. pratense. Aus allen Bestäubungen wurden Samen erhalten. 86% der Nachkommen entsprachen jedoch morphologisch und cytologisch ihrem mütterlichen Elter und waren vermutlich das Ergebnis einer Selbstbefruchtung. Viele Samen, die möglicherweise aus Kreuzungen stammten, waren klein und/oder keimten nicht. Aus einigen Samen erwuchsen schwache oder albino-Sämlinge, die bald abstarben. Griffel von T. medium, die aus Blüten herausgelöst wurden, die sich bei 40 °C geöffnet hatten, waren für Pollen von T. pratense verträglicher als Griffel, die sich auf dem Felde entwickelten. Dieser Befund läßt den Schluß zu, daß die höhere Temperatur einen besseren Hybridsamenansatz bewirkt hat, als er in unseren früheren Untersuchungen beobachtet wurde. Eine Temperaturbehandlung kann sich demnach bei Trifolium für eine Erzeugung von Hybriden als günstig erweisen.
    Notes: Summary Plants of seven perennial Trifolium species were interspecifically pollinated. The female flowers opened at 40 °C, and the male flowers opened in the greenhouse or field without a temperature treatment. T. pratense was pollinated with T. ambiguum, T. hirtum, T. ochroleucum, T. rubens, T. sarosiense, and T. medium. T. medium was pollinated with T. pratense. Seeds were obtained from all pollinations ; however, 86 of the progeny were morphologically and cytologically similar to their female parent, and probably resulted from self-fertilization. Many seeds of possible hybrid origin were small and/or did not germinate. Some seeds produced weak or albino seedlings which died at an early age. Excised pistils of T. medium from flowers that opened at 40 °C were more compatible with pollen of T. pratense than pistils of T. medium that developed in the field, indicating that the elevated temperature caused greater hybrid seed production than was observed in our earlier studies. The high temperature technique may prove useful in obtaining hybrids in Trifolium.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 41 (1971), S. 275-278 
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The excised pistil technique was used to study effects of pollen treatments on self-incompatibility in red clover. Pollen was treated directly with infrared or ultraviolet radiation, and indirectly with chemicals that were applied to the stigmas before pollination. The chemicals (boric acid, calcium nitrate, colchicine, cyclohexamide, gibberellic acid, glycine, and indole butyric acid) were each used at five concentrations. High dosage rates of most treatments inhibited pollen growth in styles that were genetically compatible, but none of the treatments had a specific effect on the self-incompatibility mechanism. The effects of growth of compatible or incompatible pollen tubes in a style on subsequent growth of both types of pollen tubes in the same style were studied in an experiment with 6 hr between pollinations. The passage of some compatible pollen tubes through the style did not influence either self- or cross-compatibility in the style after the second pollination. The passage of some self-incompatible tubes into the styles caused a slightly higher number of incompatible tubes to penetrate the style after the second pollination. Each pollen tube appeared to traverse the style independently of other tubes.
    Type of Medium: Electronic Resource
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