ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The degradation and conversion of β-sitosterol to C-17-ketosteroids byNocardia sp. M 29 was studied. Maximal enzymatic activity was found after 24 h of incubation. Although the key enzymes involved in the decomposition of β-sitosterol were inducible, no separate induction of side chain hydroxylase or 9α-hydroxylase was possible. Inhibition of the steroid ring cleaving enzyme by α,α′-dipyridyl resulted in low yields of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione (total yield 22%). Addition of lipophilic organic adsorbents (Amberlite XAD-2 and XAD-4) stimulated the 1,4-androstadiene-3,17-dione formation (maximal 50% within 120 h of incubation). Furthermore, 3-oxo-23,24-dinor-1,4-choladienic acid and 3-oxo-23,24-dinor-1,4-choladienic acid methyl ester were accumulated in the presence of Amberlite XAD-2 in moderate yields (total 11%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01278608
Permalink