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  • GEOPHYSICS  (19)
  • Cell & Developmental Biology
  • 1975-1979  (19)
  • 1965-1969  (3)
  • 1960-1964  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Glucocorticoids enhance the mitogenic action of insulin in serum-free cultures of chick embryo cells (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 98 (1979), S. 561-570 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Addition of insulin to nonproliferating serum-free cultures of secondary chicken embryo (CE) cells caused a 30% to 50% increase in cell number. Addition of any one of several glucocorticoids (dexamethasone, cortisol, or corticosterone) to the cultures two days before insulin addition increased the mitogenic effect of insulin by about twofold at each insulin concentration tested. This glucocorticoid stimulation of cell proliferation was “permissive” because in the absence of insulin glucocorticoids caused little increase in cell number (usually less than 15%). Glucocorticoids were maximally active at low concentrations (e.g., 10-10 M dexamethasone). Steroids without glucocorticoid activity were inactive over a wide range of concentrations. Glucocorticoids increased the mitogenic response to insulin largely by increasing the percentage of cells that insulin stimulated to synthesize DNA.The maximum mitogenic effect of insulin upon CE cells rapidly decreased after the cells were serially subcultured. After only nine population doublings (4 passages) in culture, the response to insulin was diminished by about 70%. The mitogenic effect of insulin plus dexamethasone declined similarly during serial subculture, and was always about twofold greater than the effect of insulin alone. The cells maintained their mitogenic responsiveness to serum as these responses decreased.In contrast to the growth promoting influence of glucocorticoids in the presence of insulin, glucocorticoids inhibited the mitogenic response of CE cells to serum. This result may resolve our above findings with reports that glucocorticoids inhibit the proliferation of CE cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040980314
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characteristics of concanavalin A-resistant Chinese hamster ovary cells and certain revertants (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 100 (1979), S. 39-54 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Clones of Chinese hamster ovary (CHO) cells were isolated by single-step selection for resistance to killing by Concanavalin A (ConA) and certain cellular and membrane properties were examined. The ConA-resistant isolates were only about 2-fold more resistant than wild type cells to the selecting lectin, but exhibited pleiotropic temperature-sensitivity for growth, markedly altered morphology and adherence, and significant differences in susceptibility to other agents such as colchicine. Two revertants to full temperature-resistance were isolated from different ConA-resistant mutants. One revertant clone had reacquired wild type sensitivity to ConA while the other revertant remained ConA-resistant. The two series of wild type, ConA-resistant, and temperature revertant clones were analyzed for altered mobility of cell surface glycoproteins using lactoperoxidase/125I and galactose oxidase/[3H]borohydride labelling procedures. The ConA-resistant clones showed increased mobility on polyacrylamide gels of three classes of labelled proteins, in the molecular weight ranges 225,000, 200,000, and 130,000 daltons. These changes persisted in the temperature-revertant that remained ConA-resistant, while two of the altered protein classes were restored to wild type mobility in the revertant that regained ConA-sensitivity. Cell hybridization experiments indicated that the temperature-sensitive phenotypes of different ConA-resistant isolates are recessive and noncomplementing, implying that the same gene is affected in each case. The reversions to temperature resistance appear to be recessive suppressor mutations in different genes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041000105
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  • 3
    Electronic Resource
    Electronic Resource
    Growth enhancement of myogenic tumor cells by conditioned medium from embryo fibroblastsThis research was supported by grants from the Cancer Council of Western Australia and the National Health and Medical Research Council. (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 321-326 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Growth medium was conditioned by incubation on mouse embryo cells in vitro. Supplementation of agar suspension cultures with conditioned medium from primary cells, but not from established lines, readily enhanced colony development by mouse tumor cells. Only cells with the properties of myoblasts responded to conditioned medium. Other fibroblastoid cells and virus-transformed cell lines were not affected. Myogenic cells in agar cultures grew in the presence of conditioned medium but did not differentiate. Soluble collagen at 400 m̈g/ml possessed little colony-stimulating activity by comparison with fresh conditioned medium.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860403
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  • 4
    Electronic Resource
    Electronic Resource
    Control of granulopoiesis in man. III. Inhibition of colony formation by dense leukocytesSupported by Grant 263, The Ontario Cancer Treatment and Research Foundation. (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 337-342 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cellular feeder layers, prepared from normal blood leukocytes, usually stimulate human marrow to form colonies. A significant increase in the stimulating activity of unseparated leukocyte feeder layers is brought about following the removal of dense leukocytes in a manner which avoids enrichment of any remaining cell type. Restoration of dense leukocytes to a dense leukocyte depleted leukocyte feeder layer results in the reduction of stimulating activity to that of an unseparated leukocyte feeder; however, addition of dense leukocytes to unseparated leukocyte feeder layers has no effect on the stimulatory activity, over the range of concentrations used in this study.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860405
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  • 5
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    Short term magnetospheric particle variations (1 minute T 1 day), appendix 1 (1979)
    In:  CASI
    Details
    Publication Date: 2006-01-16
    Description: A schematic representation of the generation and propagation processes for energetic particles of concern in solar terrestrial predictions is given. Particle precipitation at low, mid, and high altitudes is discussed with emphasis on prediction techniques. Methods given for testing of such techniques include traditional collaborations, enhanced collaborations, simulated prediction schemes, and field tests.
    Keywords: GEOPHYSICS
    Type: NASA. Marshall Space Flight Center Solar-Terrest. Predictions Proc., Vol. 2; p 433-440
    Format: text
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  • 6
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    Origin of the Cheney-Palouse Scabland tract (1978)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The Cheney-Palouse tract of the channeled scabland is the largest continuous tract of scabland in eastern Washington. The tract is composed of a varied assortment of bedrock erosional forms, loess islands, and gravel bars. Prominent bedrock longitudinal grooves and inner channels formed by macroturbulent plucking erosion of the jointed rock. Loess island forms vary as a function of their position within the flow. The three major types (submerged, partially submerged, and subaerially exposed) created sedimentologic conditions and resulting bar forms distinct from one another. Other bar forms, notably expansion bars, account for most of the sedimentation in the tract.
    Keywords: GEOPHYSICS
    Type: Texas Univ. at Austin The Channeled Scabland; p 117-130
    Format: application/pdf
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  • 7
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    Large-scale erosional and depositional features of the Channeled Scabland (1978)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The channeled scabland is a great anastomosing complex of highly overfit stand channels eroded into the basalt bedrock and overlying sediments of the Columbia Plateau. Both the erosional and depositional bed forms in these channels are described according to a simple hierarchical classification. The catastrophic flood flows produced macroforms (scale controlled by channel width) through the erosion of rock and sediment and by deposition (bars). Mesoforms (scale controlled by channel depth) are also erosional and depositional.
    Keywords: GEOPHYSICS
    Type: The Channeled Scabland; p 81-115
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  • 8
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    Quaternary geology of the Channeled Scabland and adjacent areas (1978)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The quaternary history of the channeled scabland is characterized by discrete episodes of catastrophic flooding and prolonged periods of loess accumulation and soil formation. The loess sequence was correlated with Richmond's Rocky Mountain glacial chronology. At least five major catastrophic flood events occurred in the general vicinity of the channeled scabland. The earliest episode occurred prior to the extensive deposition of the Palouse formation. The last major episode of flooding occurred between about 18,000 and 13,000 years ago. It probably consisted of two outbursts from glacial Lake Missoula.
    Keywords: GEOPHYSICS
    Type: The Channeled Scabland; p 17-35
    Format: application/pdf
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  • 9
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    Thermal conductivities of gaseous h2o, d2o, and the equimolar h2o-d2o mixture (1964)
    In:  CASI
    Details
    Publication Date: 2019-05-21
    Description: Thermal conductivities of water vapor, deuterium oxide vapor and equimolar mixture of water and deuterium oxide
    Keywords: GEOPHYSICS
    Type: NASA-RP-176
    Format: application/pdf
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  • 10
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    Substorm warnings - An ISEE-3 real time data system (1979)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: The use of solar wind measurements made by ISEE-3 in its halo orbit around the L1 libration point to predict the onsets of magnetospheric substorms and geomagnetic storms is discussed. Consideration is given to the limitations on the predictive ability of the satellite measurements set by the bulk solar wind velocity, the elliptical orbit of the satellite and the correlation lengths of the magnetic field and the solar wind plasma. The ISEE-3 real-time data system is presented, with attention given to the ground receiving stations, the NASCOM communications system, the Multisatellite Operations Control Center and Information Processing Division at the Goddard Space Flight Center, the link between Goddard and the NOAA Space Environmental Services Center, and the NOAA Space Environment Laboratory data acquisition and display data system, which includes displays allowing storm forecasts. It is noted that the entire system should be operational by March, 1980.
    Keywords: GEOPHYSICS
    Type: EOS; 60; 41, 1; 1979
    Format: text
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