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  • stem calli  (1)
  • storage protein biosynthesis  (1)
  • weight frequency  (1)
  • Springer  (3)
  • Institute of Physics
  • Nature Publishing Group
  • 1980-1984  (3)
Sammlung
Verlag/Herausgeber
  • Springer  (3)
  • Institute of Physics
  • Nature Publishing Group
Erscheinungszeitraum
  • 1980-1984  (3)
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant molecular biology 1 (1981), S. 19-34 
    ISSN: 1573-5028
    Schlagwort(e): Glycine max ; storage protein biosynthesis ; in vitro translation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The synthesis and processing of the major storage proteins in soybean cotyledons was studied both in vivo and in vitro. The α and α′ subunits of 7S as well as the 11S proteins are synthesized as higher molecular weight-precursors on membrane-bound polysomes. The initial translation products of the 7S are proteolytically cleaved during translation suggesting the removal of a ‘signal peptide’ as evidenced by the presence of 2α and 2α′ peptides immunoreactive with 7S antibody in the in vitro chain completion products of the membrane-bound polysomes. This is followed or accompanied by cotranslational glycosylation, which increases their size equivalent to that of initially-synthesized precursors. In vivo pulse-labelled 7S α and α′ products are of slightly higher molecular weights than the immunoprecipitable chain-completion products, indicating further post-translational modifications. A slow post-translational processing during a period of 1.5 to 16 h yields the final 7S α and α′ glycoproteins. Acidic and basic subunits of the 11S protein appear to be synthesized from common large molecular weight (60K-59K) precursors. Antibodies to the 11S acidic component recognize both acidic and basic domains in the precursor while those raised against basic subunits appear to be specific for that region only. The processing of the 11S precursor is also very slow and occurs post-translationally. This slow rate of processing, coupled with a temporal difference in the synthesis of 7S and 11S components, suggests a highly coordinated mechanism for synthesis and packaging of these proteins into protein bodies during seed development.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Mathematical geology 12 (1980), S. 247-265 
    ISSN: 1573-8868
    Schlagwort(e): weight frequency ; shape ; grain size ; computer application
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Mathematik
    Notizen: Abstract When grains of a sediment sample are separated by sieving into a number of size classes, the weight of the grains belonging to a particular size-class is called the weight frequency of that class. That the weight frequencies cannot be used as simple frequencies for the calculation of the mean and standard deviation of size of grains is well known. A method is developed in this paper for estimating these two as well as a third parameter, named shape parameter, by minimizing a quadratic form that arises naturally as an analogue of the χ 2 statistic. Two fully worked out numerical examples, with simulated data, are presented to illustrate the method. A computer program in FORTRAN language is also appended. Comparative study shows that the quicker conventional method used by geologists may produce reasonably good estimates of standard deviation when the sample size is large, but the estimates of mean may show large deviations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 3 (1984), S. 325-331 
    ISSN: 1573-5044
    Schlagwort(e): Dioscorea floribunda ; stem calli ; transplantable plantlets ; tuberization at nodes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Callus cultures were established from node and internode segments of Dioscorea floribunda Mart. & Gal. Both Murashige and Skoog's and modified White's medium supported callusing as well as organogenesis when supplemented with either 2,4-D or NAA in combination with BAP or Kn. On development of shoot primordia, calli were transferred to unsupplemented, half strength MS basal medium. This procedure led to the increase in formation of shoots. Several ‘crops’ of shoots were obtained from single differentiating callus cultures by excising the shoots and subculturing the residual part. Seventy percent of plantlets survived rooting and transfer to soil. When they were maintained in half-strength MS basal medium and 0.5 mg1-1 of NAA, 70% of plantlets formed aerial tubers at nodes. These tubers produced both roots and shoots and could be detached from the mother plant.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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