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  • Gas chromatography
  • Nitrogen fixation
  • Springer  (153)
  • American Association of Petroleum Geologists (AAPG)
  • Nature Publishing Group
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  • 1
    Electronic Resource
    Electronic Resource
    Accumulation of the phytoalexin, glyceollin, in root nodules of soybean formed by effective and ineffective strains ofBradyrhizobium japonicum (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 997-1008 
    Details
    ISSN: 1573-1561
    Keywords: Nitrogen fixation ; glyceollin ; phytoalexin ; Bradyrhizobium japonicum ; nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Nitrogen fixation in root nodules formed by strain 2143 ofBradyrhizobium japonicum andGlycine max (L.) Merr. cv Williams 82 reaches a maximum at 21 to 28 days postinoculation and then begins to decline. The phytoalexin, glyceollin, accumulates in nodules coincident with the decline in nitrogen fixation. Nodules formed by strain 3122, which are unable to fix nitrogen, accumulate even higher levels of glyceollin and do so beginning 21 days postinoculation even though these nodules contain no recoverable bacteria. The typical phytoalexin response occurs within days of infection. The mechanism by which this response in theBradyrhizobium japonicum-soybean combination is delayed 2 to 3 weeks after infection is presently unknown but phytoalexin accumulation could contribute to the inability of the soybean-Bradyrhizobium japonicum combination to maintain high levels of nitrogen fixation throughout the growing season.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00980058
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  • 2
    Electronic Resource
    Electronic Resource
    Utilization of nitrate by bacteroids of Bradyrhizobium japonicum in the soybean root nodule (1988)
    Springer
    Planta 174 (1988), S. 51-58 
    Details
    ISSN: 1432-2048
    Keywords: Bacteroid ; Bradyrhizobium ; Glycine (N2 fixation) ; Nitrate reductase ; Nitrite reductase ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacteroids of Bradyrhizobium japonicum strain CB1809, unlike CC705, do not have a high level of constitutive nitrate reductase (NR; EC 1.7.99.4) in the soybean (Glycine max. Merr.) nodule. Ex planta both strains have a high activity of NR when cultured on 5 mM nitrate at 2% O2 (v/v). Nitrite reductase (NiR) was active in cultured cells of bradyrhizobia, but activity with succinate as electron donor was not detected in freshly-isolated bacteroids. A low activity was measured with reduced methyl viologen. When bacteroids of CC705 were incubated with nitrate there was a rapid production of nitrite which resulted in repression of NR. Subsequently when NiR was induced, nitrite was utilized and NR activity recovered. Nitrate reductase was induced in bacteroids of strain CB1809 when they were incubated in-vitro with nitrate or nitrite. Increase in NR activity was prevented by rifampicin (10 μg· ml-1) or chloramphenicol (50 μg·ml-1). Nitrite-reductase activity in bacteroids of strain CB1809 was induced in parallel with NR. When nitrate was supplied to soybeans nodulated with strain CC705, nitrite was detected in nodule extracts prepared in aqueous media and it accumulated during storage (1°C) and on further incubation at 25°C. Nitrite was not detected in nodule extracts prepared in ethanol. Thus nitrite accumulation in nodule tissue appears to occur only after maceration and although bacteroids of some strains of B. japonicum have a high level of a constitutive NR, they do not appear to reduce nitrate in the nodule because this anion does not gain access to the bacteroid zone. Soybeans nodulated with strains CC705 and CB1809 were equally sensitive to nitrate inhibition of N2 fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394873
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  • 3
    Electronic Resource
    Electronic Resource
    Chemically modified teflon capillary columns for gas chromatography II. Solid surface effects in direct coated columns (1982)
    Springer
    Chromatographia 15 (1982), S. 641-644 
    Details
    ISSN: 1612-1112
    Keywords: Teflon capillary columns ; Chemically modified teflon ; Gas chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The preparation of teflon capillary columns employing direct coating of the stationary phase to the chemically modified teflon surface and the role of the chemically modified teflon in the separation mechanism are described. Two types of contributions from the modified teflon have been observed: nonspecific adsorption of the carbon surface and specific interactions of polar groups in the carbon skeleton. The use of polar liquid phases can eliminate adsorption due to the presence of polar groups in the modified teflon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279491
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of two granular nematicides on growth and nodulation ofArachis hypogea L. (1982)
    Springer
    Plant and soil 66 (1982), S. 413-416 
    Details
    ISSN: 1573-5036
    Keywords: Growth ; Nematicide ; Nitrogen fixation ; Nodulation ; Peanut ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of two granular nematicidesviz. oxamyl and fenamiphos, on the nodulation and growth of Rhizobium inoculatedArachis hypogaea L. was studied in glasshouse and field trials. In the glasshouse trial at the suggested rates of application shoot fresh weight was significantly reduced by oxamyl whilst root fresh weight was similarly affected by fenamiphos. In the field trial vegetative growth and plant emergence were significantly reduced by both nematicides. Nodulation at the higher rates of application was increased by both oxamyl and fenamiphos whilst oxamyl caused a significant increase in pod number at the highest rate of application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02183808
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  • 5
    Electronic Resource
    Electronic Resource
    Cross-inoculation specificity ofPsophocarpus tetragonolobus (L.) DC (1981)
    Springer
    Plant and soil 60 (1981), S. 139-142 
    Details
    ISSN: 1573-5036
    Keywords: Legumes ; Nitrogen fixation ; Nodulation ; Winged beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Only legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpus tetragonolobus) were found to form nodules in a temperate zone soil with no previous history of legume cropping. Isolates from root nodules from uninoculated winged beans grown in the field only nodulated legumes in the cowpea cross-inoculation group.Rhizobium japonicum formed ineffective nodules with the winged bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02377119
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  • 6
    Electronic Resource
    Electronic Resource
    Delayed inoculation and starter nitrogen for enhancing early growth and nitrogen status ofLespedeza cuneata (1985)
    Springer
    Plant and soil 84 (1985), S. 311-321 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Minesoil ; Nitrogen fixation ; Nitrogen source ; Reclamation ; Sericea lespedeza ; Surface mining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two growth chamber experiments were conducted to determine the response ofLespedeza cuneata (Dumont) G. Don. (sericea lespedeza) to delayed inoculation and low levels of nitrogen fertilization. Nitrogen was supplied either as NH 4 + or as NO 3 − in solution. At 0.5 and 5.0 ppm nitrogen early growth and N2(C2H2) fixation was inhibited by NH 4 + and promoted by NO 3 − . Inoculation at seeding did not negatively affect growth prior to the onset of N2(C2H2) fixation. Delayed inoculation until the trifoliate stage thus did not increase growth or N2 fixation during the first 40 days of growth. After 40 days, specific nitrogenase activity was highest for plants inoculated at the first trifoliate stage of growth. In contrast, growth and total shoot nitrogen accumulation were higher in plants inoculated at planting. The experimental results suggest that delaying inoculation is not a useful technique for improving early growth ofL. cuneata for surface mine reclamation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02275471
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  • 7
    Electronic Resource
    Electronic Resource
    GC/MSD detection of the metabolic residues of metolachlor in tomato fruit (1992)
    Springer
    Chromatographia 33 (1992), S. 147-150 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; MS detection ; Metachlor ; Herbicide residues ; Tomatoes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A gas chromatograph/mass selective detector method is presented for the analysis of the major metabolic residues of metolachlor by acid hydrolysis of tomato fruit. The two major hydrolysis products, CGA-37913 (2-[2-ethyl-6-methylphenyl]amino-1-propanol) and derivatised CGA-49751 (4-[2-ethyl-6-methylphenyl]-2-hydroxy-5-methyl-3-morpholinone) were monitored in the selective ion mode atm/z 162 andm/z 160, respectively. Recoveries from 87 to 96% were obtained for tomato fortified at 50 to 200μg kg−1. The proposed method of analysis will allow for a detection limit of 11 μg kg−1 for CGA-37913 and 12 μg kg−1 for the derivatised CGA-49751.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02275895
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  • 8
    Electronic Resource
    Electronic Resource
    Trace determination of amines and other nitrogen containing compounds with a modiefied thermal energy analyzer (TEATM) (1982)
    Springer
    Chromatographia 16 (1982), S. 354-358 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Modified thermal energy analyzer ; Selective detection of nitrogen compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A modification to the TEATM Analyzer is described, which allows it to be used as a highly selective GC detector for nitrogen compounds as well as for N-nitrosamines and nitro compounds. The modified Analyzer is as sensitive as the AFID, but has a much higher selectivity towards nitrogen compounds than the AFID. Its response appears to be molar, with an output that is dependent only on the number of nitrogen atoms present. The performance of the modified Analyzer for the analysis of ammonia, amines,N-nitrosamines, nitriles and organonitro compounds is reported and discussed, as is its application to the detection of these compounds in bacon and beer samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258937
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  • 9
    Electronic Resource
    Electronic Resource
    Determination of acids in Brazilian sugar cane spirits and other alcoholic beverages by HRGC-SPE (1998)
    Springer
    Chromatographia 48 (1998), S. 751-757 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Solid phase extraction ; Brazilian sugar cane spirit ; Lower fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A gas chromatographic method for the analysis of seventeen acids in a water/ethanol matrix is described. The diluted samples were first concentrated by solid phase extraction. Nonanoic acid was the internal standard. A good separation profile was obtained for all compounds, including the isomers: n and iso-butyric acids, n and isovaleric acids and n and iso-caproic acids. The method is fast, sensitive (20–30 μg L−1) and has a good reproducibility (4–7 %). The recovery was in the range of 94%–116% for the medium chain acids (C7-C16). This method allowed the identification of fourteen acids in caninhas and other spirits. The total acid content and the profile of the exportation type caninhas are similar to the ones obtained for several international beverages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02467643
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  • 10
    Electronic Resource
    Electronic Resource
    Modification of supports with liquid phases by thermal treatment in a closed system (1985)
    Springer
    Chromatographia 20 (1985), S. 228-230 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Modified diatomaceous supports
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Diatomaceous earth type supports are modified with a liquid phase, by high-temperature thermal treatment in a closed system. It is shown that the retention characteristics of the support changes by this treatment. The main advantage of this method, as compared with modification in a flow-through system is that liquid phase evaporation and possibility of oxydation during the process can be avoided. This ensures a better uniformity of the modified support.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02259692
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