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  • Chemistry  (94)
  • Inorganic Chemistry  (40)
  • Methanol  (3)
  • 42.75
  • 1980-1984  (46)
  • 1925-1929  (51)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 131 (1982), S. 174-175 
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Methanol ; Glucose ; Methanol dissimilating enzymes ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparative study was made of the regulation of the synthesis of methanol dissimilating enzymes inkloeckera sp. 2201 andHansenula polymorpha using chemostat and batch growth conditions and methanol or glucose as carbon sources. During growth in methanol-limited chemostat cultures similar enzyme patterns for alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase in the two yeasts were found. When growing in batch culture with glucoseH. polymorpha, but notKloeckera sp. 2201, was found to produce ethanol which might affect the synthesis of these enzymes.
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  • 2
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Chemostat ; Mixed substrates ; Glucose ; Methanol ; Growth yields ; Enzyme regulation ; Dissimilatory enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of the composition of methanol/glucose-mixtures as only sources of carbon and energy on growth and regulation of the synthesis of enzymes involved in methanol-dissimilation was studied under chemostat conditions at a fixed dilution rate with the methylotrophic yeasts Hansenula polymorpha and Kloeckera sp. 2201. Both carbon sources were found to be utilized completely independently of the composition of the C1/C6 mixture. Using mixtures of 14C-labelled methanol and glucose the growth yield for glucose was found to be constant for all C1/C6-mixtures tested and both yeasts. The growth yield for methanol, however, was reduced by up to 25% when the proportion of methanol in the inflowing medium was lower than 20% (w/w with respect to glucose) for H. polymorpha and 50% (w/w with respect to glucose) for Kloeckera sp. 2201 respectively. During growth with C1/C6-mixtures containing higher C1-proportions of methanol regular growth yields for methanol were recorded which corresponded to the growth yields found with methanol as the only carbon source. The regulation of the synthesis of the enzymes of the dissimilatory pathway for methanol was found to be under multiple control. Although glucose was present in the medium methanol had a positive effect on the synthesis of these enzymes. Thus, in addition to derepression induction by methanol was also observed. This inductive effect was found to increase with increasing proportions of methanol in the mixture. Depending on the enzyme, 10–40% methanol in the mixture resulted in a maximal induction with enzyme specific activities equal to those found in cells grown with methanol as the only carbon source. No further enhancements in enzyme specific activities were observed during growth on mixtures containing more than 40% methanol.
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  • 3
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Kloeckera sp. 2201 ; Mixed substrate utilisation ; Chemostat ; Induction ; Repression ; Methanol ; Glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of Hansenula polymorpha and Kloeckera sp. 2201 with a mixture of glucose and methanol (38.8%/61.2%, w/w) and the regulation of the methanol dissimilating enzymes alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase were studied in chemostat culture, as a function of the dilution rate. Both organisms utilized and assimilated glucose and methanol simultaneously up to dilution rates of 0.30 h-1 (H. polymorpha) and 0.26h-1, respectively (Kloeckera sp. 2201) which significantly exceeded μmax found for the two yeasts with methanol as the only source of carbon. At higher dilution rates methanol utilisation ceased and only glucose was assimilated. Over the whole range of mixed-substrate growth both carbon sources were assimilated with the same efficiency as during growth with glucose or methanol alone. In cultures of H. polymorpha, however, the growth yield for glucose was lowered by the unmetabolized methanol at high dilution rates. During growth on both carbon sources the repression of the synthesis of all catabolic methanol enzymes which is normally caused by glucose was overcome by the inductive effect of the simultaneously fed methanol. In both organisms the synthesis of alcohol oxidase was found to be regulated differently as compared to catalase, formaldehyde and formate dehydrogenase. Whereas increasing repression of the synthesis of alcohol oxidase was found with increasing dilution rates as indicated by gradually decreasing specific activities of this enzyme in cell-free extracts, the specific activities of this enzyme in cell-free extracts, the specific activities of catalase and the dehydrogenases increased with increasing growth rates until repression started. The results indicate similar patterns of the regulation of the synthesis of methanol dissimilating enzymes in different methylotrophic yeasts.
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  • 4
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 11 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 182 (1929), S. 97-117 
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 8 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 152 (1926), S. 185-196 
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 298-298 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Closing technique for static coating ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 11 (1980), S. 209-212 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: X-Ray Stress Measurement on Austenitic Steels - A Possibility of Predicting Fatigue DamageBased on the results found for fatigue tests on a ferritic steel combined with x-ray stress analysis similar investigations were carried out for an austenitic stainless steel. For that reason the Wöhler tests were interrupted after defined load cycle numbers and the residual stress and the half value breadth of the x-ray peaks were determined in the groove of the samples. The investigation has shown correlations between residual stress, load amplitudes and load cycle numbers. These relations in connection with a dependence of the breadth of the x-ray peaks from the load cycle number have been used for a non-destructive testing model of predicting the life of samples under alternating load.
    Notes: Aufbauend auf Untersuchungen an einem ferritischen Stahl wurden an Proben aus einem austenitischen Stahl Ermüdungsversuche kombiniert mit Eigenspannungsmessungen durchgeführt. Dazu wurden die Wöhlerversuche definiert nach vorgegebenen Lastwechselzahlen unterbrochen und röntgenografisch die Eigenspannungen und Interferenzbreiten im Kerb der Proben bestimmt. Es zeigten sich Abhängigkeiten der Oberflächeneigenspannungen von Lastamplituden und Lastwechselzahlen, die unter Zuhilfenahme der ebenfalls gefundenen Zusammenhänge zwischen Halbwertbreiten und Lastwechselzahlen dazu herangezogen werden, um auf zerstörungsfreiem Wege auf die Lebensdauer wechselbeanspruchter Proben zu schließen.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 15 (1984), S. 299-308 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: The Effect of Moisture on the Mechanical Properties of Phenolic Moulded MaterialsIn order to use thermosetting moulding material it is necessary to know how the mechanical properties are changed by absorption of water. Therefore, multiphase systems based on phenolic resin, with varying quantities of wood flour and/or powdered minerals as fillers, were examined. The relationship between moisture absorption and the change in Young's modulus, flexural strength and the strain of the outer fibre at fracture was determined. It could be shown that the increase in flexural strength as water absorption began is a result only of the swelling stress.
    Notes: Voraussetzung für den Einsatz duroplastischer Formstoffe in erhöhter Umgebungsfeuchte ist die Kenntnis der durch die Feuchteaufnahme bewirkten Änderung der mechanischen Werkstoffeigenschaften. Ausgehend von in ihrer Zusammensetzung variierten Mehrstoffsystemen auf der Basis Phenolharz/Holzmehl/Gesteinsmehl wird die formstoff- und feuchtespezifische Wechselwirkung zwischen Feuchteaufnahme, Biege-E-Modul, Biegefestigkeit und Randfaserdehnung beim Bruch aufgezeigt, und nachgewiesen, daß der zu Lagerungsbeginn zu beobachtende Anstieg der Biegefestigkeit ausschließlich auf durch Sorption hervorgerufene Druck-Eigenspannungen zurückzuführen ist.
    Additional Material: 6 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 15 (1984), S. 349-359 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: The Swelling Behaviour of Thermosetting MaterialsThe moisture absorption of moulded materials can endanger a construction because of the change in volume of various parts. Therefore it is necessary to know the maximum possible swelling of the moulded materials employed at different relative humidities. The swelling of modified phenolic resin systems are described. Wood flour (which absorbs moisture) and powdered minerals (which in this case can be considered as inert) were used as fillers.Different methods have been developed to calculate the maximum possible swelling after a relatively short testing time (ca. 1000 h).
    Notes: Die Feuchteaufnahme eines Formstoffes kann durch die damit verbundene Volumenänderung des Formteils die Funktion einer Baugruppe gefährden. Daher muß die maximal mögliche formstoff- und umgebungsfeuchtespezifische Quelldehnung der eingesetzten Formstoffe bekannt sein. Am Beispiel von Ein-, Zwei- und Dreistoffsystemen mit Phenolharz als Matrixwekstoff wird der Einfluß der Harzträger Holzmehl (im Hinblick auf die Feuchteaufnahme „aktiv“) und Gesteinsmehl („inaktiv“) auf die Quellung untersucht. In diesem Zusammenhang werden Methoden aufgezeigt, die eine Abschätzung der maximal möglichen Quelldehnung nach relativ kurzer Versuchsdauer (etwa 1000 h) ermöglichen.
    Additional Material: 6 Ill.
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