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  • Analytical Chemistry and Spectroscopy  (13)
  • COMPOSITE MATERIALS  (6)
  • Golgi apparatus  (3)
  • 1980-1984  (17)
  • 1975-1979  (5)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Light and electron microscopic cytochemistry of the epidermal mucoid cells ofAeolidia papillosa andCoryphella rufibranchialis (Mollusca, Nudibranchia) (1983)
    Springer
    Protoplasma 114 (1983), S. 14-22 
    Details
    ISSN: 1615-6102
    Keywords: Cytochemistry ; Epidermis ; Golgi apparatus ; Mucoid cells ; Nudibranchia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal mucoid cells in the cerata ofAeolidia papillosa andCoryphella rufibranchialis were cytochemically tested to determine the composition of their secretory products. The PAS, alcian blue, PA-TSC-SP and high iron diamine stains were used to determine the presence of acidic, sulphated, and periodate-reactive groups on the mucopolysaccharides. The secretory granules in mucoid cells ofA. papillosa consisted of periodate-reactive mucopolysaccharides with acid groups other than sulphates. Each granule also contained fibrils which were not oxidized by periodic acid. The mucoid secretory granules inC. rufibranchialis contained weakly acidic sulphated mucopolysaccharides, but no periodate-reactive polysaccharide groups. In both aeolid nudibranchs the Golgi apparatus at the base of the mucoid cells contained products which stained the same as those in the secretory granules, confirming that the Golgi apparatus is involved in the synthesis of mucoid secretory products. In addition the mucoid cell Golgi complex ofC. rufibranchialis also gave rise to ellipsoid vacuoles which contained sulphated mucopolysaccharides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279864
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  • 2
    Electronic Resource
    Electronic Resource
    The Golgi apparatus in epidermal mucoid and ellipsoid-vacuolate cells ofAeolidia papillosa andCoryphella rufibranchialis (Nudibranchia) (1980)
    Springer
    Protoplasma 102 (1980), S. 217-233 
    Details
    ISSN: 1615-6102
    Keywords: Epidermis ; Golgi apparatus ; Nudibranchia ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The epidermal cell layer of the apical end of the ceras was investigated in two species of aeolid nudibranchs. Based on cellular inclusions, mostly two cell types were found: mucoid and ellipsoid-vacuolate cells. Mucoid cells ofCoryphella rufibranchialis have large heterogeneous and fibrillar secretory granules whereas inAeolidia papillosa, the granules are homogeneous, but vary in electron density from one cell to another. Ellipsoid-vacuolate cells contained large quantities of small vacuoles with an included ellipsoidal structure. Both species contained very numerous ellipsoid-vacuolate cells. Secretory granules and ellipsoid-vacuoles appear to arise from the Golgi apparatus and these contents stain with PAS, suggesting a polysaccharide composition. Mucoid cells contained both secretory granules and ellipsoid-vacuoles which may arise from the same Golgi apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279589
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  • 3
    Electronic Resource
    Electronic Resource
    Biochemical and ultrastructural studies of ectoglycosyltransferase systems of murine L1210 leukemic cells (1978)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 8 (1978), S. 139-152 
    Details
    ISSN: 0091-7419
    Keywords: sialyltransferase ; galactosyltransferase ; electron microscope autoradiography ; plasma membrane ; Golgi apparatus ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Intact murine L1210 leukemic cells incorporated significant quantities of [3H]-N-acetylneuraminic acid directly from CMP-N-acetylneuraminic acid. When pretreated with Vibrio cholerae neuraminidase, incorporation increased sixfold to tenfold. Biochemical studies comparing incorporation of N-acetyl-neuraminic acid from the nucleotide sugar with that from free sugar demonstrated that the relatively high levels of incorporation from CMP-N-acetyl-neuraminic acid could not be due to the incorporation of free sugar generated by extracellular degradation of the nucleotide sugar. Very little N-acetylneuraminic acid was taken up or incorporated by L 1210 cells from free sugar and this incorporation was not increased by neuraminidase pretreatment. Moreover, extracellular breakdown of CMP-N-acetylneuraminic acid during incubations with L 1210 cells was rather insignificant.Electron microscope autoradiography of cells incubated with CMP-N-acetylneuraminic acid demonstrated that greater than 84% of the incorporated radioactivity was associated with the plasma membrane and less than 1% with the Golgi apparatus. These findings are consistent with the conclusion that incroporation of N-acetylneuraminic acid from CMP-N-acetylneuraminic acid is the consequence of a cell surface sialytransferase system. Pretreatment of cells with the nonpenetrating reagent, diazonium salt of sulfonilic acid, significantly inhibited this ectoenzyme system while only marginally affecting galactose uptake and incorporation at the Golgi apparatus. Interestingly, incorporation from CMP-N-acetylneuraminic acid declined as the viability of the cell population declined. When taken together, the above evidence develops a rigorous argument for the presence of a sialyltransferase enzyme system at the cell surface of L 1210 cells.Studies directed towards the detection of a similar ectogalactosyltransferase system were also undertaken. Cells incubated in the presence of UDP-[3H]-galactose incorporated radioactivity into a macromolecular fraction. The presence of excess unlabeled galactose in the incubation medium significantly reduced this incorporation. Electron microscope autoradiographs of cells incubated with UDP-[3H]-galactose, demonstrated that incorporation occurred primarily at the Golgi apparatus. The grain distribution in these autoradiographs was similar to that for free galactose. Thus, the incorporation observed for L-1210 cells incubated in UDP-[3H]-galactose was due primarily to the intracellular utilization of free galactose generated by extracellular degradation of the nucleotide sugar. Inability t o demonstrate an ectogalacto-syltransferase system on L1210 cells does not rule out the possibility that the enzyme is present but undetectable due t o the absence of appropriate cell surface acceptor molecules.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400080204
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  • 4
    Electronic Resource
    Electronic Resource
    On the formation of artefact peaks in linked scans of the magnet and electric sector fields in a mass spectrometer (1977)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1977), S. 735-738 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The apperrance of peaks at non-integral masses in linked scans, where the ratio B/E is kept constant, is shown to be caused by fragmentations of metastable ions occuring in the region between the magnetic and electric sectors. A formula is given for calculating the mass of the parent metastable ion and that of its daughter ion.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210121206
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  • 5
    Electronic Resource
    Electronic Resource
    The elimination of carbon monoxide from molecular ions of phenol and other [C6H6O]+· isomers (1982)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 17 (1982), S. 81-85 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Phenol and five acyclic isomeric compounds have been investigated using electron impact and field ionization techniques, mass analysed ion kinetic energy spectrometry and collision induced dissociation mass analysed ion kinetic energy spectrometry. The kinetic energy release data corresponding to the elimination of carbon monoxide from the molecular ions show that at least two structures of the reactant ion are involved. The electron impact and field ionization collision induced dissociation mass analysed ion kinetic energy spectra indicate that under electron impact conditions, the phenol ion partially isomerizes to another ion structure. An isomerization of about 40% to the structure of cis-2-hexen-4-yn-1-al is in good accordance with the spectral data.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210170206
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  • 6
    Electronic Resource
    Electronic Resource
    The structures of [C5H5O]+ ions formed from isomers of nitrophenol in the gaseous phase (1982)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 17 (1982), S. 331-334 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Translational energy release measurments on metastable ions are used in the comparison of the structures of isomeric ions. Metastable ions, m2+, formed from m1+ ions as the result of a high energy process in the ion source are compared with isomeric metastable ions formed as daughters from fragmentation of metastable m1+ ions in a field. In the case of o-, m- and p-nitrophenol the structure of the [C5H5O]+ ions formed from [C6H5O]+ ions by these two independent methods is different as verified by comparison of the behaviour of [C5H5O]+ ions formed from several other compounds.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210170709
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  • 7
    Electronic Resource
    Electronic Resource
    The modern mass spectrometer - a complete chemical laboratory (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 16 (1981), S. 101-114 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Using nitrobenzene as an example, various ways in which a contemporary mass spectrometer can be utilized to yield a wealth of information about the compound studied are reviewed. Applying a variety of different techniques and procedures, in addition to the conventional low resolution mass spectrum, the following nitrobenzene spectra have been obtained: collision induced dissociation mass spectrum, mass analysed ion kinetic energy spectra, collision induced dissociation mass analysed ion kinetic energy spectra, spectra obtained at constant B/E, spectra obtained at constant B2/E, high voltage scans of metastable ion fragmentation processes, consecutive fragmentations in different field free regions, charge exchange mass spectra, charge stripping mass spectra, doubly charged ion mass spectra, chemical ionization mass spectra, negative ion mass spectra, negative ion mass analysed ion kinetic energy spectra, negative ion mass analysed ion kinetic energy collision induced dissociation spectra, charge inversion spectra, etc. The complementary types of information available from the above studies are discussed to show the unique versatility of mass spectrometry as a technique for the examination of organic compounds.
    Additional Material: 32 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210160302
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  • 8
    Electronic Resource
    Electronic Resource
    Ammonia in the ion source. 1-Negative ion chemical ionization of phenols1 (1984)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1984), S. 199-199 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210190413
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  • 9
    Electronic Resource
    Electronic Resource
    Studies of consecutive reactions of quinones in a reversed geometry mass spectrometer (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 16 (1981), S. 312-322 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Consecutive reactions involving the ejection of molecules of carbon monoxide from quinone systems have been studied. Using a reversed geometry double focusing mass spectrometer, the individual steps of a consecutive reaction may be separated into different field free regions. The structure of ions formed in the ion source may be compared with those formed from the fragmentation of metastable ions in a field free region by studying differences in the pattern of translational energies released when they break. In the case of anthraquinone it is found that two structures exist for the [M—CO]+· ion. Critical energy measurements strengthen this argument. Furthermore, 18O labelling has been used to distinguish between the loss of carbon monoxide from different positions on the molecular ion of 1-hydroxyanthraquinone.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210160710
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  • 10
    Electronic Resource
    Electronic Resource
    Freon-113 as a reagent gas in the negative chemical ionization of phenols and acidic fractions of coal-derived liquids1 (1984)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1984), S. 583-584 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210191112
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